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Registered Laboratory Animal Technician (RLAT), RLAT Certification, Complete RLAT Exam Practice Questions and Answers..

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Registered Laboratory Animal Technician (RLAT), RLAT Certification, Complete RLAT Exam Practice Questions and Answers

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autoclave Packs on an open shelf have ____ the safe storage life as packs in a closed
cabinet.


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half




Describe Pseudocapillaria in zebrafish (caused by, transmission, clinical signs,
prevention/treatment)


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, nematode disease by P.toementosa
transmitted by ingestion of eggs of infected fish
Clinical signs= emaciation, decreased reproduction
Treatment= fenbendazole, ivermectin, emamectin benzoate, mebendazole,
praziquantel




describe the types of handling/restraint for the following species:
-mouse
-rat
-rabbit
-guinea pig
-hamster
-gerbil
-ferret


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-mouse: scruff & cupping
-rat: "v" restraint, "criss cross", towel burrito
-rabbit: football hold (support hind end)
-guinea pig: over shoulders with fingers in front and behind forelimb
-hamster: scoop/cup
-gerbil: scoop/cup
-ferret: WITH GLOVES, hands across shoulder and push forelimbs together;
other hand support hind end




Alimentary canal


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mouth, pharynx, esophagus, stomach, small intestine, large intestine, anus

,Class II BSC Type B1


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Personnel, Environment and Product Protection
50% air exhausted, 50% recirculated
Hard duct exhaust
can use trace amounts of vapours, chemicals, isotopes




What is MS-222?


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Buffered TMS
TMS (400g) + sodium bicarbonate (1g) + 100mL DI water




prevent disease outbreak: DONT's (9)


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- prop open doors
- lift microisolator lids w/o proper ventilation
- refill water bottles (replace instead)
- put jumpers back in cage
- move from dirty to clean areas
- use same instruments w/o disinfecting between
- swap enrichment devices w/o disinfecting
- save food from hoppers during cage cleans
- keep rodents as pets if you are working in a disease-free rodent facility

, What are the natural habitats of zebrafish?


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warm, slow-moving water (ponds, pools, ditches, lakes, rice paddies




Vitamin E


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prevents free radical damage to tissues


Selenium= constituent of glutathione peroxidase which breaks down
peroxide
Vit E= antioxidant that prevents formation of peroxides

Deficiency= nutritional myopathy (white muscle disease)




Refinement


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Modify husbandry or experimental procedures to minimize pain/distress
and enhance welfare of animal




Natural Ingredient Diet


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