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Registered Laboratory Animal Technician (RLAT), RLAT Certification, Complete RLAT Exam Practice Questions and Answers..

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This document contains a comprehensive collection of Registered Laboratory Animal Technician (RLAT) exam practice questions and answers presented in a flashcard-style format. It covers laboratory animal husbandry, animal welfare, biosafety, biosecurity, anesthetic equipment, nutrition, disease prevention, zebrafish management, microbiology, regulations, containment levels, research procedures, and species-specific care. The material is designed to reinforce key RLAT concepts through exam-style review, making it an excellent resource for certification preparation and final revision. It also includes detailed content on aquatic laboratory animal management, laboratory safety standards, and Canadian biosafety guidelines.

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Describe a sick zebrafish


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skinny, hunched spine, blood present




Neuroleptanalgesics


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Combination of tranquilizer (neuroleptic) and opioid




How many zebrafish strains are there?

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more than 43,000




Potassium


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carb metabolism, maintenance of intra and extracellular osmolality and pH,
myocardial function, higher concentrations intracellularly (RBCs have 25x
more potassium than blood plasma)




Magnesium


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metabolism (co-enzymes)



Acute deficiency causes Grass tetany/staggers in cattle,
Chicks require 8x the magnesium of a young rat




How do you distinguish between female and male domesticated zebrafish?


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, Female= large/round whitish abdomen, pale yellow anal fin
Males= flat yellowish abdomen, dark yellow anal fin




MPV stands for


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Mice Parvovirus




What does Herpes B virus cause?


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Infection of CNS




Secondary containment barrier


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physical plant features that prevent entry or escape from work
environment, e.g. animal housing room or lab areas, doors, exhaust, SOPs




Vitamin K


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, Coagulation (activates prothrombin)
Abundant in plants, synthesized in gut
Non-herbivores need dietary source, stored in body tissues
Water-soluble synthetic form= menadione




Herbivores


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GPs, rabbits, sheep
Have larger GI tract to breakdown plant/fibre for energy through
fermentation
Microorganisms in GI tract to synthesize nutrients for host animals
Teeth made for grinding food




N95 does not protect against


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chemical fumes or other toxic vapors




Natural host of the Herpes B virus


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All members of the genus Macaca (non-human primates)

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