BIO250L LAB 1 MICROBIOLOGY FULL LAB
REPORT GUIDE WITH ASEPTIC TECHNIQUE
AND MICROSCOPE USE
◉ If your cells look washed out under the scope what can you do to
fix the lighting.
Answer: Adjust the lighting by using diaphragm or condenser
◉ Binocular versus monocular.
Answer: Binocular has two eyepieces. Monocular has one eyepiece.
◉ Inorder to increase the resolution under the 100X oil objective,
you must open this part of the microscope..
Answer: diaphragm
◉ path of light through a microscope and tell what happens after the
light passes through each part..
Answer:
◉ Eukaryote.
Answer: have a nucleus, membrane bound organelles and are larger
than prokaryotes
, ◉ Prokaryote.
Answer: no nucleus, no membrane bound organelles and are small
◉ If you went from the 40X objective to the 100X objective and the
cell you were looking at disappeared, give several explanations
explaining what could have happened and how you could fix the
problem..
Answer: Field of vision narrowed—center specimen
Not enough light—open diaphragm
Slightly out of focus—use fine focus
The lens is not clicked all the way in place
◉ Cellular morphology.
Answer: cell shape
◉ 3 types of cell shapes CBS.
Answer: coccus (spheres), bacillus (rods), and spirillum (spiral)
◉ Field of vision.
Answer: area you can see, smaller at higher powers; must CENTER
object or you will lose it at higher power
REPORT GUIDE WITH ASEPTIC TECHNIQUE
AND MICROSCOPE USE
◉ If your cells look washed out under the scope what can you do to
fix the lighting.
Answer: Adjust the lighting by using diaphragm or condenser
◉ Binocular versus monocular.
Answer: Binocular has two eyepieces. Monocular has one eyepiece.
◉ Inorder to increase the resolution under the 100X oil objective,
you must open this part of the microscope..
Answer: diaphragm
◉ path of light through a microscope and tell what happens after the
light passes through each part..
Answer:
◉ Eukaryote.
Answer: have a nucleus, membrane bound organelles and are larger
than prokaryotes
, ◉ Prokaryote.
Answer: no nucleus, no membrane bound organelles and are small
◉ If you went from the 40X objective to the 100X objective and the
cell you were looking at disappeared, give several explanations
explaining what could have happened and how you could fix the
problem..
Answer: Field of vision narrowed—center specimen
Not enough light—open diaphragm
Slightly out of focus—use fine focus
The lens is not clicked all the way in place
◉ Cellular morphology.
Answer: cell shape
◉ 3 types of cell shapes CBS.
Answer: coccus (spheres), bacillus (rods), and spirillum (spiral)
◉ Field of vision.
Answer: area you can see, smaller at higher powers; must CENTER
object or you will lose it at higher power
