CG(ASCP) Exam
1. The most important reason why biological safety to protect the technologist
cabinets are used instead of horizontal laminar flow who is processing the spec-
hoods in the handling of human tissues is imens
2. In which of the following situations would one sus- culture media is turbid in
pect bacterial contamination appearance
3. Which of the following chemicals is used for cryop- dimethyl sulfoxide (DMSD)
reservation?
4. Which of the following is a buffer used in cultures? HEPES
5. In order for the fragile X to be expressed in affect- folic acid deficient
ed individuals, lymphocytes must be grown in what
type of media?
6. The following enzymes cab be used to dissociate RNAse
Chronic Villus samples (CVS) except for
7. Which of the following is not a necessary ingredient antibiotics
which is added to modified medium?
8. All of the following are factors which affect the cry-
opreservation except
9. A fast growing culture shows signs of cell death. cell confluency: too many or
What is a possible reason for this? too few cells
10. collagenase, pronase, and trypsin are used to dis- proteolytic enzymes
perse tissue into single-cell suspension. These sub-
stances are:
11. colcemid
, CG(ASCP) Exam
When harvesting peripheral blood cultures, asceptic
technique no longer matters following incubation
with:
12. Which of the following culture processes would be mitomycin-C stress test of
most useful for identifying patients w/ fanconi ane- peripheral blood
mia?
13. To avoid potential culture failure, it is important to expiration date
monitor the reagents used. Recording which of the
following information will be most important when
documenting culture failure?
14. The morning after being set-up, a single dish of am- harvest the culture as soon
niotic fluid culture turns cloudy and appears conta- as possible
minated. None of the other cultures set up that day
appear contaminated. Which of the following should
be done first?
15. If an amniotic fluid culture from the same sample incubator failure
grows well in a different incubator, the probable
cause of the culture failure is
16. Positive staining w/ Hoechst 33258 reveals micronu- mycoplasma
clei. Which of the following agents caused the cul-
ture contamination?
17. Which of the following statements is true about is is useful in removing cells
trypsin> from culture flasks
18. Which of the following is the best tissue to use for chorionic villi
both a good success rate and abnormality detection
in spontaneous abortion specimen
, CG(ASCP) Exam
19. When a culture becomes contaminated, the culture yellow
medium shifts to an acidic pH. If the medium con-
tains a pH indicator, the color of the medium in the
flask will appear
20. Which of the following reagents is used in culturing phytohemagglutinin (PHA)
peripheral blood, but not in culturing bone marrow
or amniotic fluid or chromosome analysis?
21. Which of the following is the best agent to use for BM cycline
the presence of mycoplasma growth in a culture?
22. A bone marrow culture on a patient w/ APL M3 has too much time in transit
failed to yield analyzable metaphases. The culture
had a cell density of 1x10^4 calls/ml. The specimen
had been in transit for 2 days and was not sent in
a transport medium. The most likely cause of the
failure is:
23. Which of the following microscopes should be used dissection
when cleaning a chorionic villus specimen?
24. Blood specimen for chromosome analysis may be 96 hours
kept in culture up to how many hours after culture
initiation?
25. which of the following cell types is diagnostically maternal decidua
most problematic if present in a CVS sample?
26. Cells from a skin fibroblast culture are to be frozen 5
for storage in liquid nitrogen. The cell concentration
is 2X10^7 cells/ml. How many 2ml vials can be frozen
, CG(ASCP) Exam
if the final cell concentration for storage is to be
2X10^6?
27. The time that a flask culture takes to reach confluen- humidity of the culture envi-
cy depends on all of the following except the ronment
28. Which of the following is true regarding closed-sys- there is less chance of mi-
tem cultures? crobial contamination
29. A blood culture for a patient diagnosed w/ Di-George that an insufficient number
syndrome fails to grow in culture. The most likely of cells were seeded in the
cause of the failure is: culture
30. To maximize the effectiveness of the biological safe- move his or her arms in and
ty cabinet, the technologist should out slowly, perpendicular to
the opening
31. The following solution is used to detach monolayer Trypsin- EDTA
cells
32. Which of the following will help prevent microbial aseptic technique
and cross contamination of cultures?
33. Which of the following factors are not important for mitotic index
chromosome spreading?
34. A good banding pattern will be expected when we dark
observe chromosomes before banding under the
phase scope with the following appearance:
35. Karyotype with a del 5q bad prognosis, frequently
associated with MDS
1. The most important reason why biological safety to protect the technologist
cabinets are used instead of horizontal laminar flow who is processing the spec-
hoods in the handling of human tissues is imens
2. In which of the following situations would one sus- culture media is turbid in
pect bacterial contamination appearance
3. Which of the following chemicals is used for cryop- dimethyl sulfoxide (DMSD)
reservation?
4. Which of the following is a buffer used in cultures? HEPES
5. In order for the fragile X to be expressed in affect- folic acid deficient
ed individuals, lymphocytes must be grown in what
type of media?
6. The following enzymes cab be used to dissociate RNAse
Chronic Villus samples (CVS) except for
7. Which of the following is not a necessary ingredient antibiotics
which is added to modified medium?
8. All of the following are factors which affect the cry-
opreservation except
9. A fast growing culture shows signs of cell death. cell confluency: too many or
What is a possible reason for this? too few cells
10. collagenase, pronase, and trypsin are used to dis- proteolytic enzymes
perse tissue into single-cell suspension. These sub-
stances are:
11. colcemid
, CG(ASCP) Exam
When harvesting peripheral blood cultures, asceptic
technique no longer matters following incubation
with:
12. Which of the following culture processes would be mitomycin-C stress test of
most useful for identifying patients w/ fanconi ane- peripheral blood
mia?
13. To avoid potential culture failure, it is important to expiration date
monitor the reagents used. Recording which of the
following information will be most important when
documenting culture failure?
14. The morning after being set-up, a single dish of am- harvest the culture as soon
niotic fluid culture turns cloudy and appears conta- as possible
minated. None of the other cultures set up that day
appear contaminated. Which of the following should
be done first?
15. If an amniotic fluid culture from the same sample incubator failure
grows well in a different incubator, the probable
cause of the culture failure is
16. Positive staining w/ Hoechst 33258 reveals micronu- mycoplasma
clei. Which of the following agents caused the cul-
ture contamination?
17. Which of the following statements is true about is is useful in removing cells
trypsin> from culture flasks
18. Which of the following is the best tissue to use for chorionic villi
both a good success rate and abnormality detection
in spontaneous abortion specimen
, CG(ASCP) Exam
19. When a culture becomes contaminated, the culture yellow
medium shifts to an acidic pH. If the medium con-
tains a pH indicator, the color of the medium in the
flask will appear
20. Which of the following reagents is used in culturing phytohemagglutinin (PHA)
peripheral blood, but not in culturing bone marrow
or amniotic fluid or chromosome analysis?
21. Which of the following is the best agent to use for BM cycline
the presence of mycoplasma growth in a culture?
22. A bone marrow culture on a patient w/ APL M3 has too much time in transit
failed to yield analyzable metaphases. The culture
had a cell density of 1x10^4 calls/ml. The specimen
had been in transit for 2 days and was not sent in
a transport medium. The most likely cause of the
failure is:
23. Which of the following microscopes should be used dissection
when cleaning a chorionic villus specimen?
24. Blood specimen for chromosome analysis may be 96 hours
kept in culture up to how many hours after culture
initiation?
25. which of the following cell types is diagnostically maternal decidua
most problematic if present in a CVS sample?
26. Cells from a skin fibroblast culture are to be frozen 5
for storage in liquid nitrogen. The cell concentration
is 2X10^7 cells/ml. How many 2ml vials can be frozen
, CG(ASCP) Exam
if the final cell concentration for storage is to be
2X10^6?
27. The time that a flask culture takes to reach confluen- humidity of the culture envi-
cy depends on all of the following except the ronment
28. Which of the following is true regarding closed-sys- there is less chance of mi-
tem cultures? crobial contamination
29. A blood culture for a patient diagnosed w/ Di-George that an insufficient number
syndrome fails to grow in culture. The most likely of cells were seeded in the
cause of the failure is: culture
30. To maximize the effectiveness of the biological safe- move his or her arms in and
ty cabinet, the technologist should out slowly, perpendicular to
the opening
31. The following solution is used to detach monolayer Trypsin- EDTA
cells
32. Which of the following will help prevent microbial aseptic technique
and cross contamination of cultures?
33. Which of the following factors are not important for mitotic index
chromosome spreading?
34. A good banding pattern will be expected when we dark
observe chromosomes before banding under the
phase scope with the following appearance:
35. Karyotype with a del 5q bad prognosis, frequently
associated with MDS
