WGU BIOCHEMISTRY OA LATEST COMPREHENSIVE EXAMS QUESTIONS AND ANSWERS SURE

WGU BIOCHEMISTRY OA LATEST COMPREHENSIVE
EXAMS QUESTIONS AND ANSWERS SURE A+
✔✔Match the letters with the correct names of the processes of the central dogma
(Replication; Transcription; Translation).

1. A) Transcription, B) Translation, C) Replication
2. A) Translation, B) Replication, C) Transcription
3. A) Replication, B) Transcription, C) Translation
4. A) Translation B)Transcription, Replication - ✔✔3. A) Replication, B) Transcription, C)
Translation

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Correct! The central dogma starts with DNA, which is able to be replicated to prepare
for cell division. DNA in the nucleus can also be accessed and transcribed to mRNA.
The mRNA will leave the nucleus and enter the cytoplasm where it will be translated
into a protein by the ribosome.

✔✔Label the lettered strands of nucleic acid below. The possible words to use in
labeling strands are: Coding Strand, Non-template Strand, Non-coding Strand,
Template Strand, and mRNA. Note: A and B refer to one strand of DNA; C and D refer
to the other strand of DNA.

,1. A) Template B) Coding C) Non-template D) Non-Coding E) mRNA
2. A) Template B) Non-coding C) Non-template D) Coding E) mRNA
3. A) Non-template B) Non-Coding C) Template D) Coding E) mRNA
4. A) Non-template B) Coding C) Template D) Non-coding E) mRNA - ✔✔4. A) Non-
template B) Coding C) Template D) Non-coding E) mRNA

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Correct! The coding and the non-template strands are the same strand (blanks A and
B). The non-coding and the template strands are the same strand (blanks C and D).

✔✔If one strand of chromosome 2 has a DNA sequence that consists of this: 5' AAG
CGG TAC GTA 3' What will be the composition of the complementary DNA strand?
(Select all that apply)

a. 5' TTC GCC ATG CAT 3'
b. 3' TTC GCC ATG CAT 5'
c. 5' TAC GTA CCG CTT 3'
d. 3' AAG CGG TAC GTA 5' - ✔✔b. 3' TTC GCC ATG CAT 5'
c. 5' TAC GTA CCG CTT 3'

Feedback
Correct! All complementary base pairing must be antiparallel. The complementary
strand to 5' AAG CGG TAC GTA 3' is 3' TTC GCC ATG CAT 3'. If we simply 'flip' the
sequence, we get 5' TAC GTA CCG CTT 3'. Thus, these are the correct answers.

✔✔During DNA replication, which of the following sequences can be used as a primer
for the following DNA sequence: 3' AGT GGA TCA CTA GGC TCT 5'? (Recall that DNA
replication uses RNA primers whereas PCR uses DNA primers).

5' UCA CCU AGU GAU 3'
5' TCA CCT AGT GAT 3'
3' UCA CCU AGU GAU 5'
3' TCA CCT AGT GAT 5' - ✔✔5' UCA CCU AGU GAU 3'

Feedback
Correct! During DNA replication, RNA is used as a primer for the DNA polymerase.
Recall that primers are complementary and antiparallel to the strand of DNA that is
being copied. The complementary RNA sequence for 3' AGT GGA TCA CTA GGC TCT
5'? is 5' UCA CCU AGU GAU CCG AGA 3'. Thus 5' UCA CCU AGU GAU 3', which is
contained within the sequence above (beginning at the 5' end), could serve as a
complementary primer for DNA synthesis.

✔✔Which of the following is the correct tRNA anticodon for the mRNA codon 5' GCA 3'
?

5' UGC 3'

,5' CGU 3'
5' TGC 3'
5' CGT 3' - ✔✔5' UGC 3'

Feedback
Correct. mRNA binding to tRNA is also complementary and antiparallel.

✔✔The following sequence is the coding DNA strand of the collagen gene: 5' ATG GCG
TTC GAA 3' What is the sequence of the corresponding mRNA?

a. 3' ATG GCG TTC GAA 5'
b. 5' AUG GCG UUC CUU 3'
c. 5' AUG GCG UUC GAA 3'
d. 5' UTG GCG TTC GUU 3' - ✔✔c. 5' AUG GCG UUC GAA 3'

Feedback
Correct. The coding strand and the mRNA both go in the same direction, but do not
both contain Ts.

✔✔What would be the resulting mRNA sequence from a template strand with this
sequence: 5'-CAG CTC GTC-3'?

a. 5'-GUC GAG CAG-3'
b. 5'-GAC GAG CUG-3'
c. 3'-GAC GAG CUG-5'
d. 3'-GUG GAG GAG-5' - ✔✔b. 5'-GAC GAG CUG-3'

Feedback
Correct! Sequences that bind each other (are complementary) must also be antiparallel
(running in opposite directions). The sequence that is complementary to 5'-CAG CTC
GTC-3' is 3'-GUC GAG CAG-5'. Since the possible answers in the list to choose from
are presented with their 5' ends on the left, we simply 'flip' the sequence to get 5'-GAC
GAG CUG-3'.

✔✔What is the coding strand sequence if the non-template strand sequence is 5'-AGC
CTT TAA CTA-3'

a. 5'-TCG GAA ATT GAT-3'
b. 5'-TAG TTA AAG GCT-3'
c. 3'-AGC CTT TAA CTA-5'
d. 3'-ATC AAT TTC CGA-5' - ✔✔d. 3'-ATC AAT TTC CGA-5'

Feedback
Correct! The names "coding strand" and "non-template strand" refer to the exact same
piece of DNA. Therefore, if the non-template strand is 5'-AGC CTT TAA CTA-3', then

, the coding strand is exactly the same sequence (because it is the same piece of DNA:
3'-ATC AAT TTC CGA-5'.

✔✔What would be the amino acid sequence that would result from this template
sequence: 5'-TGC AAG CCA-3'?

a. Pro Leu Trp
b. Thr Phe Gly
c. Gly Phe Thr
d. Trp Leu Ala - ✔✔d. Trp Leu Ala

Feedback
Correct! The RNA polymerase enzyme makes mRNA by antiparallel and
complementary basepairing with the template strand of DNA. Therefore, the mRNA
transcribed from this template would be 3'—ACG UUC GGU-5'. mRNA codons are read
5' --> 3' on the genetic code table. 'Flipping the sequence gives us 5'-UGG CUU GCA-
3'. Using the genetic code table, we see that UGG encodes Trp; CUU encodes Leu; and
GCA encodes Ala.

✔✔What would the amino acid sequence be from this coding strand sequence: 5'-GGA
AGG CCC-3'?

a. Gly Arg Pro
b. Lys Pro Ser
c. Pro Gly Arg
d. Val Ala Pro - ✔✔a. Gly Arg Pro

Feedback
Correct! The mRNA transcribed from the template strand is complementary and
antiparallel to it. The coding strand of DNA (also called the non-template strand) is also
complementary and antiparallel to the template strand. Thus, translating 5'-GGA AGG
CCC-3' gives us Gly Arg Pro

✔✔Proteins that promote coiling of DNA and help prevent DNA strands from "tangling"
are called?

Histones
Enzymes
Polymerase
Chaperones - ✔✔Histones

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Correct! The combination of DNA and histone proteins creates nucleosomes.