TIETZ FUNDAMENTALS OF CLINICAL
CHEMISTRY AND MOLECULAR
DIAGNOSTICS EIGHTH EDITION NADER
RIFAI TEST BANK SOLVED QUESTIONS
WITH VERIFIED RESPONSES FULL
SOLUTION GRADED A+
⩥ 2. In mass spectrometry, the sum of all ions produced is displayed as a
function of time to yield a(n): a. product ion. b. extracted ion profile. c.
mass spectrum. d. total ion chromatogram.
Answer: Answer: d. total ion chromatogram.
⩥ 3. Which type of ionization technique uses an electron beam to ionize
the reagent gas and produce a reactive species as a result of ion-
molecule reactions? a. Electron b. Chemical c. Electrospray d.
Inductively coupled plasma
Answer: Answer: b. Chemical
⩥ 4. Inductively coupled plasma mass spectrometry is typically used for:
a. determination of trace elements. b. identification of a protein. c. drug
identification. d. isotopic labeling of molecules.
Answer: Answer: a. determination of trace elements.
,⩥ 5. The most widely used ionization approach coupled with gas
chromatography and used for comprehensive drug screening is: a.
electrospray ionization. b. chemical ionization. c. MALDI. d. electron
ionization.
Answer: Answer: d. electron ionization.
⩥ 6. Currently, the most widely used mass spectrometer, which is
flexible, easy to use, and of relatively low cost, is the: a. quadrupole
mass spectrometer. b. time-of-flight instrument. c. magnetic sector mass
spectrometer. d. linear ion trap mass spectrometer.
Answer: Answer: a. quadrupole mass spectrometer.
⩥ Question
Answer: Answer
⩥ Review Questions:
Answer:
⩥ 1. The Michaelis-Menten constant is: a. Vmax. b. Km. c. 1/v. d. [S].
Answer: Answer: b. Km.
⩥ 2. The "active center" of an enzyme is: a. the protein part of an
enzyme without the cofactor necessary for catalysis. b. that part of an
enzyme that diminishes the rate of a chemical reaction. c. a site other
, than the substrate-binding site. d. that part of an enzyme at which
substrate binding occurs.
Answer: Answer: d. that part of an enzyme at which substrate binding
occurs.
⩥ 3. Which statement(s) below is (are) correct? a. Apoenzyme +
Prosthetic group = Holoenzyme b. An enzyme without the prosthetic
group attached is referred to as an apoenzyme. c. A prosthetic group is a
coenzyme permanently bound to an enzyme molecule. d. All of the
above statements are correct.
Answer: Answer: d. All of the above statements are correct.
⩥ 4. The graph on which the plot of 1/velocity versus 1/substrate
concentration is illustrated is referred to as a: a. rate plot. b. Lineweaver-
Burk plot. c. maximum velocity plot. d. Michaelis-Menten plot.
Answer: Answer: b. Lineweaver-Burk plot.
⩥ 5. An enzyme assay during which a single measurement is taken at the
termination of the enzymatic reaction is referred to as a: a. fixed-time
assay. b. terminator assay. c. kinetic assay. d. continuous-monitoring
assay.
Answer: Answer: a. fixed-time assay.
⩥ 6. A reactant in a catalysis reaction that binds to the enzyme's active
site is the: a. enzyme. b. product. c. substrate. d. coenzyme.
CHEMISTRY AND MOLECULAR
DIAGNOSTICS EIGHTH EDITION NADER
RIFAI TEST BANK SOLVED QUESTIONS
WITH VERIFIED RESPONSES FULL
SOLUTION GRADED A+
⩥ 2. In mass spectrometry, the sum of all ions produced is displayed as a
function of time to yield a(n): a. product ion. b. extracted ion profile. c.
mass spectrum. d. total ion chromatogram.
Answer: Answer: d. total ion chromatogram.
⩥ 3. Which type of ionization technique uses an electron beam to ionize
the reagent gas and produce a reactive species as a result of ion-
molecule reactions? a. Electron b. Chemical c. Electrospray d.
Inductively coupled plasma
Answer: Answer: b. Chemical
⩥ 4. Inductively coupled plasma mass spectrometry is typically used for:
a. determination of trace elements. b. identification of a protein. c. drug
identification. d. isotopic labeling of molecules.
Answer: Answer: a. determination of trace elements.
,⩥ 5. The most widely used ionization approach coupled with gas
chromatography and used for comprehensive drug screening is: a.
electrospray ionization. b. chemical ionization. c. MALDI. d. electron
ionization.
Answer: Answer: d. electron ionization.
⩥ 6. Currently, the most widely used mass spectrometer, which is
flexible, easy to use, and of relatively low cost, is the: a. quadrupole
mass spectrometer. b. time-of-flight instrument. c. magnetic sector mass
spectrometer. d. linear ion trap mass spectrometer.
Answer: Answer: a. quadrupole mass spectrometer.
⩥ Question
Answer: Answer
⩥ Review Questions:
Answer:
⩥ 1. The Michaelis-Menten constant is: a. Vmax. b. Km. c. 1/v. d. [S].
Answer: Answer: b. Km.
⩥ 2. The "active center" of an enzyme is: a. the protein part of an
enzyme without the cofactor necessary for catalysis. b. that part of an
enzyme that diminishes the rate of a chemical reaction. c. a site other
, than the substrate-binding site. d. that part of an enzyme at which
substrate binding occurs.
Answer: Answer: d. that part of an enzyme at which substrate binding
occurs.
⩥ 3. Which statement(s) below is (are) correct? a. Apoenzyme +
Prosthetic group = Holoenzyme b. An enzyme without the prosthetic
group attached is referred to as an apoenzyme. c. A prosthetic group is a
coenzyme permanently bound to an enzyme molecule. d. All of the
above statements are correct.
Answer: Answer: d. All of the above statements are correct.
⩥ 4. The graph on which the plot of 1/velocity versus 1/substrate
concentration is illustrated is referred to as a: a. rate plot. b. Lineweaver-
Burk plot. c. maximum velocity plot. d. Michaelis-Menten plot.
Answer: Answer: b. Lineweaver-Burk plot.
⩥ 5. An enzyme assay during which a single measurement is taken at the
termination of the enzymatic reaction is referred to as a: a. fixed-time
assay. b. terminator assay. c. kinetic assay. d. continuous-monitoring
assay.
Answer: Answer: a. fixed-time assay.
⩥ 6. A reactant in a catalysis reaction that binds to the enzyme's active
site is the: a. enzyme. b. product. c. substrate. d. coenzyme.
