TEXTBOOK OF DIAGNOSTIC
MICROBIOLOGY SIXTH EDITION CONNIE R
MAHON TEST BANK SOLVED QUESTIONS
WITH VERIFIED RESPONSES FULL
SOLUTION GRADED A+
⩥ What can immunological methods detect besides whole microbes?
Answer: Microbial antigens or host antibodies against microbes.
⩥ Why are immunological methods useful for unculturable organisms?
Answer: Because identification can be based on antigen-antibody
reactivity rather than growth in culture.
⩥ What is biotyping?
Answer: Identification of bacteria using biochemical tests based on
metabolic and fermentation patterns.
⩥ What does TSI stand for?
Answer: Triple Sugar Iron.
⩥ What is contained in TSI medium according to lecture?
, Answer: Low glucose, a mixture of disaccharides, protein digest
(casein/peptones), inorganic iron, and a pH indicator.
⩥ What is the purpose of TSI testing?
Answer: To help identify gram-negative bacteria based on carbohydrate
fermentation and hydrogen sulfide production.
⩥ What part of a TSI tube is the slant and what part is the butt?
Answer: The slanted aerobic surface is the slant; the deeper anaerobic
portion is the butt.
⩥ What does blackening in a TSI tube indicate?
Answer: Hydrogen sulfide production (FeS formation).
⩥ What commercial biochemical identification kit for gram-negative
bacteria was shown?
Answer: The API biotyping test by bioMerieux.
⩥ Why are biochemical tests usually performed on pure colonies?
Answer: Mixed cultures can give misleading metabolic results.
⩥ What does PCR detect in microbiology?
Answer: Microbial nucleic acids, usually DNA.
MICROBIOLOGY SIXTH EDITION CONNIE R
MAHON TEST BANK SOLVED QUESTIONS
WITH VERIFIED RESPONSES FULL
SOLUTION GRADED A+
⩥ What can immunological methods detect besides whole microbes?
Answer: Microbial antigens or host antibodies against microbes.
⩥ Why are immunological methods useful for unculturable organisms?
Answer: Because identification can be based on antigen-antibody
reactivity rather than growth in culture.
⩥ What is biotyping?
Answer: Identification of bacteria using biochemical tests based on
metabolic and fermentation patterns.
⩥ What does TSI stand for?
Answer: Triple Sugar Iron.
⩥ What is contained in TSI medium according to lecture?
, Answer: Low glucose, a mixture of disaccharides, protein digest
(casein/peptones), inorganic iron, and a pH indicator.
⩥ What is the purpose of TSI testing?
Answer: To help identify gram-negative bacteria based on carbohydrate
fermentation and hydrogen sulfide production.
⩥ What part of a TSI tube is the slant and what part is the butt?
Answer: The slanted aerobic surface is the slant; the deeper anaerobic
portion is the butt.
⩥ What does blackening in a TSI tube indicate?
Answer: Hydrogen sulfide production (FeS formation).
⩥ What commercial biochemical identification kit for gram-negative
bacteria was shown?
Answer: The API biotyping test by bioMerieux.
⩥ Why are biochemical tests usually performed on pure colonies?
Answer: Mixed cultures can give misleading metabolic results.
⩥ What does PCR detect in microbiology?
Answer: Microbial nucleic acids, usually DNA.
