Biol 200 Unit 5 Exam with Accurate
Solutions
Endomembrane system made of - correct Answer-Endoplasmic recticulum, Golgi
apparatus, Lysosome, Endosomes.
Endoplasmic Recticulum (i) and Golgi Apparatus function (ii) - correct Answer-(i)
synthesis of most lipids, synthesis of proteins for distribution to many organelles and to
plasma membrane (ii) modification, sorting, and packaging or proteins and lipids for
either secretion or delivery to another organelle
Lysosome (i) and Endosomes function (ii) - correct Answer-(i) intracellular digression (ii)
sorting of endocytosed material
Were does all translation start? - correct Answer-All translation starts in the cytoplasm
Where are ribosomes found and are the free? - correct Answer-Ribosomes are found in
the cytoplasm, some are free and some are bound to the ER
Protein Folding in the ER: Chaperone proteins - correct Answer-Prevent misfolded or
partially assembled proteins from trafficking through the endomembrane system,
Chaperones present in the ER help proteins fold properly and make sure that multi-
subunit proteins are all properly assembled
Misfolded proteins in the ER lumen trigger - correct Answer-the production of
Chaperones: the unfolded protein response
Signal-Swap Experiment shows - correct Answer-that signal sequences are necessary
and sufTicient to direct proteins to ER
New phospholipids are synthesized by - correct Answer-enzymes in the cytoplasmic
face of the SER
Asymmetry of phospholipids is established - correct Answer-Scramblase or Flippase
enzymes
Principal process throughout the endomembrane system: (think vesicles) - correct
Answer-Transport Vesicles carry the cargo, and bud from a Donor compartment and
fuse with a Target compartment
, Transport Vesicles carry the correct cargo from - correct Answer-the donor
compartment to the target compartment.
This requires - correct Answer-Selection and concentration of the vesicle content: the
correct cargo MUST get into the transport vesicle at the donor compartment, Targeting:
the transport vesicle MUST fuse with the correct destination compartment membrane
Vesicle Transport/Traffic involves four stages - correct Answer-Formation: budding off
with cargo from donor compartment - this includes the selection and concentration of
the vesicle content. Transport: movement from donor to target compartment. Docking:
arriving and making contact with the correct target compartment. Fusion: releasing
cargo into the target compartment.
Role of Vesicle Coats - correct Answer-Clathrin Coated: coat proteins are clathrin and
adaptin 1, origin is golgi, destination is lysosome
Clathrin coated 2: coat proteins are clathrin and adoption 2, origin is plasma membrane
and destination is endosome.
COP coated: coat proteins are COP proteins, origin is ER, golgi cisternia, golgi
apparatus and destination is ER, golgi cisternia, golgi apparatus
General arrangement of vesicle protein coats (Non-cytosolic side) - correct Answer-
Soluble Cargo bind cargo: receptors in the donor membrane (the cargo- receptors
interact with adaptor proteins on their cytosolic domain). Membrane Cargo: often
interact directly with adaptor proteins on their cytosolic domain.
General arrangement of vesicle protein coats (cytosolic side) - correct Answer-Cargo
receptor or membrane cargo interact with adaptor proteins, Adaptor proteins interact
with coat proteins.
Vesicle Formation Stages - correct Answer-Coat assembly and Cargo selection, Bud
formation and Pinching off, Coat-shedding
COPII-Coated Vesicles - correct Answer-Primarily involved in ER to Golgi traffic,
Dynamin-like protein not required.
COPI-Coated Vesicles - correct Answer-Primarily involved in Golgi to ER traffic
, Dynamin-like protein not required.
Catherin-Coated Vesicles - correct Answer-Primarily involved in endocytosis and
receptor recycling, Dynamin is required for budding of these vesicles.
Vesicle fusion to the Target membrane - correct Answer-depends on surface markers
Rab-proteins and SNAREs - correct Answer-Proteins that direct vesicles to the right
place are and Proteins that mediate vesicle fusion are
Solutions
Endomembrane system made of - correct Answer-Endoplasmic recticulum, Golgi
apparatus, Lysosome, Endosomes.
Endoplasmic Recticulum (i) and Golgi Apparatus function (ii) - correct Answer-(i)
synthesis of most lipids, synthesis of proteins for distribution to many organelles and to
plasma membrane (ii) modification, sorting, and packaging or proteins and lipids for
either secretion or delivery to another organelle
Lysosome (i) and Endosomes function (ii) - correct Answer-(i) intracellular digression (ii)
sorting of endocytosed material
Were does all translation start? - correct Answer-All translation starts in the cytoplasm
Where are ribosomes found and are the free? - correct Answer-Ribosomes are found in
the cytoplasm, some are free and some are bound to the ER
Protein Folding in the ER: Chaperone proteins - correct Answer-Prevent misfolded or
partially assembled proteins from trafficking through the endomembrane system,
Chaperones present in the ER help proteins fold properly and make sure that multi-
subunit proteins are all properly assembled
Misfolded proteins in the ER lumen trigger - correct Answer-the production of
Chaperones: the unfolded protein response
Signal-Swap Experiment shows - correct Answer-that signal sequences are necessary
and sufTicient to direct proteins to ER
New phospholipids are synthesized by - correct Answer-enzymes in the cytoplasmic
face of the SER
Asymmetry of phospholipids is established - correct Answer-Scramblase or Flippase
enzymes
Principal process throughout the endomembrane system: (think vesicles) - correct
Answer-Transport Vesicles carry the cargo, and bud from a Donor compartment and
fuse with a Target compartment
, Transport Vesicles carry the correct cargo from - correct Answer-the donor
compartment to the target compartment.
This requires - correct Answer-Selection and concentration of the vesicle content: the
correct cargo MUST get into the transport vesicle at the donor compartment, Targeting:
the transport vesicle MUST fuse with the correct destination compartment membrane
Vesicle Transport/Traffic involves four stages - correct Answer-Formation: budding off
with cargo from donor compartment - this includes the selection and concentration of
the vesicle content. Transport: movement from donor to target compartment. Docking:
arriving and making contact with the correct target compartment. Fusion: releasing
cargo into the target compartment.
Role of Vesicle Coats - correct Answer-Clathrin Coated: coat proteins are clathrin and
adaptin 1, origin is golgi, destination is lysosome
Clathrin coated 2: coat proteins are clathrin and adoption 2, origin is plasma membrane
and destination is endosome.
COP coated: coat proteins are COP proteins, origin is ER, golgi cisternia, golgi
apparatus and destination is ER, golgi cisternia, golgi apparatus
General arrangement of vesicle protein coats (Non-cytosolic side) - correct Answer-
Soluble Cargo bind cargo: receptors in the donor membrane (the cargo- receptors
interact with adaptor proteins on their cytosolic domain). Membrane Cargo: often
interact directly with adaptor proteins on their cytosolic domain.
General arrangement of vesicle protein coats (cytosolic side) - correct Answer-Cargo
receptor or membrane cargo interact with adaptor proteins, Adaptor proteins interact
with coat proteins.
Vesicle Formation Stages - correct Answer-Coat assembly and Cargo selection, Bud
formation and Pinching off, Coat-shedding
COPII-Coated Vesicles - correct Answer-Primarily involved in ER to Golgi traffic,
Dynamin-like protein not required.
COPI-Coated Vesicles - correct Answer-Primarily involved in Golgi to ER traffic
, Dynamin-like protein not required.
Catherin-Coated Vesicles - correct Answer-Primarily involved in endocytosis and
receptor recycling, Dynamin is required for budding of these vesicles.
Vesicle fusion to the Target membrane - correct Answer-depends on surface markers
Rab-proteins and SNAREs - correct Answer-Proteins that direct vesicles to the right
place are and Proteins that mediate vesicle fusion are
