1|Page
MICROBIOLOGY EXAM 1 (CONNECT MCGRAW
HILL) ACTUAL EXAM PREP 2026 ALL
QUESTIONS AND CORRECT DETAILED
ANSWERS ALREADY A GRADED WITH EXPERT
FEEDBACK |NEW AND REVISED
Which represent the 5 I's of culturing microorganisms? - ANSWER-
Inoculation
Incubation
Isolation
Inspection
Identification
Patient specimen is spread onto several types of culture media. -
ANSWER- inoculation
Culture media are placed at the proper growth temperature and allowed
time to grow. - ANSWER- incubation
A colony is spread onto a new plate of media using a quadrant streak
method. - ANSWER- isolation
Colonies are closely observed and Gram stains are prepared and
examined microscopically - ANSWER- inspection
,2|Page
A variety of biochemical tests are used to characterize a microbe. -
ANSWER- identification
The terms "sterile" and "pure" can be used interchangeably when
discussing cultures, because they both refer to a culture that has not been
contaminated. - ANSWER- false
Choose the three basic ways that media are classified. - ANSWER-
purpose, chemical state, chemical composition
Choose the description that best fits a medium that has been designed to
support the growth of MRSA (methicillin-resistant Staphylococcus
aureus) while inhibiting all species and strains of other bacteria -
ANSWER- selective
Select the type of media where the chemical composition is precisely
known. - ANSWER- defined
The apparent increase in size of an image - ANSWER- magnification
The ability to be distinguished from the surroundings - ANSWER-
contrast
The ability to distinguish between two separate structures that are very
close to one another - ANSWER- resolution
, 3|Page
What differential stain is most important in the diagnosis of
tuberculosis? - ANSWER- acid fast stains
True or False: Bacteria are larger than human cells. - ANSWER- false
True or False: Bacteria are only visible with an electron microscope. -
ANSWER- false
The term used to describe a cluster of spherical bacteria is _______. -
ANSWER- staphylococci
What general type of stain is used to separate types of bacteria based on
their cellular structures? - ANSWER- differential
Why must fresh cells be used when performing a Gram stain? -
ANSWER- Old cells may not Gram stain properly
After Gram's iodine is added, what color do the cells appear under a
light microscope? - ANSWER- all cells appear purple
The differential stage of the Gram stain is the application of _______. -
ANSWER- ethanol
If the Gram's iodine step was skipped in Gram stain procedure, what
color would the cells likely be when viewed under the microscope? -
ANSWER- Most cells would appear red/pink
MICROBIOLOGY EXAM 1 (CONNECT MCGRAW
HILL) ACTUAL EXAM PREP 2026 ALL
QUESTIONS AND CORRECT DETAILED
ANSWERS ALREADY A GRADED WITH EXPERT
FEEDBACK |NEW AND REVISED
Which represent the 5 I's of culturing microorganisms? - ANSWER-
Inoculation
Incubation
Isolation
Inspection
Identification
Patient specimen is spread onto several types of culture media. -
ANSWER- inoculation
Culture media are placed at the proper growth temperature and allowed
time to grow. - ANSWER- incubation
A colony is spread onto a new plate of media using a quadrant streak
method. - ANSWER- isolation
Colonies are closely observed and Gram stains are prepared and
examined microscopically - ANSWER- inspection
,2|Page
A variety of biochemical tests are used to characterize a microbe. -
ANSWER- identification
The terms "sterile" and "pure" can be used interchangeably when
discussing cultures, because they both refer to a culture that has not been
contaminated. - ANSWER- false
Choose the three basic ways that media are classified. - ANSWER-
purpose, chemical state, chemical composition
Choose the description that best fits a medium that has been designed to
support the growth of MRSA (methicillin-resistant Staphylococcus
aureus) while inhibiting all species and strains of other bacteria -
ANSWER- selective
Select the type of media where the chemical composition is precisely
known. - ANSWER- defined
The apparent increase in size of an image - ANSWER- magnification
The ability to be distinguished from the surroundings - ANSWER-
contrast
The ability to distinguish between two separate structures that are very
close to one another - ANSWER- resolution
, 3|Page
What differential stain is most important in the diagnosis of
tuberculosis? - ANSWER- acid fast stains
True or False: Bacteria are larger than human cells. - ANSWER- false
True or False: Bacteria are only visible with an electron microscope. -
ANSWER- false
The term used to describe a cluster of spherical bacteria is _______. -
ANSWER- staphylococci
What general type of stain is used to separate types of bacteria based on
their cellular structures? - ANSWER- differential
Why must fresh cells be used when performing a Gram stain? -
ANSWER- Old cells may not Gram stain properly
After Gram's iodine is added, what color do the cells appear under a
light microscope? - ANSWER- all cells appear purple
The differential stage of the Gram stain is the application of _______. -
ANSWER- ethanol
If the Gram's iodine step was skipped in Gram stain procedure, what
color would the cells likely be when viewed under the microscope? -
ANSWER- Most cells would appear red/pink
