SCYM (ASCP) Quizlet ACTUAL EXAM
WITH ACCURATE QUESTIONS AND
ANSWERS (VERIFIED ANSWERS) |
LATEST (2026/2027) UPDATED
VERSION | 100% GUARANTEED PASS
{JUST RELEASED}
1. Hydrodynamic Focusing - ANSWER ✓ Most modern flow cytometers tightly
position the sample for optical analysis via hydrodynamic focusing. Here, a
carrier fluid called the sheath fluid is used to position the sample of cells into a
single file for optical interrogation.
2. Hydordynamic focusing and sheath fluids - ANSWER ✓ The central stream
(sample stream) is focused and surrounded by the secondary slower stream
(sheath fluid). The shape and size of the flow cell is crucial to hydrodynamic
focusing, and traditionally the cell is nozzle shaped. ... In a flow cytometer, the
sheath fluid pressure is constant while the sample fluid is adjusted
3. Sample Pressure and the Sheath Pressure - ANSWER ✓ The difference
between the sample pressure and the sheath pressure is the differential
pressure. This controls the width of the core stream and the total number of
cells passing the laser intercept.
,4. differential pressure based flow cytometers - ANSWER ✓ Differential pressure
based flow cytometers currently dominate the market. These systems have two
pressure regulators. The first is at a constant pressure that sets how fast the
fluids runs at. The second is regulated by the investigator (like on this LSR-II
control panel).
5. Generation of differential pressure (syringe pump, pressure based) - ANSWER
✓ Low differential pressure allows the cells to move past the interrogation
point one at a time. .... One kind involves generating pressure using a pump
and regulator system ... Differential pressure based fluidic system. ...
peristaltic and/or syringe pumps to deliver the sample into the instrument.
6. Characterization of syringe-pump-driven induced pressure - ANSWER ✓ In
syringe-pump-driven microfluidic systems, pressure fluctuations are observed
in an elastic microchannel. The syringe pump is driven by an electrical stepper
motor, from which mechanical oscillations are expected to generate flow-rate
fluctuations and in turn leads to the pressure fluctuations in the channel flow.
7. Optical Filters - ANSWER ✓ Filters are pieces of glass coated on both sides
that allow light of a certain collection, or band, of wavelengths to pass through
while absorbing or interfering with photons of other wavelengths. These come
in bandpass, longpass, and shortpass flavors
, 8. Band Pass Optical Filter - ANSWER ✓ A filter that allows light between a set
wavelength to pass through and reflects light above and below the set
wavelength. For example, a bandpass filter with a wavelength of 550/40nm
would allow light between 530nm and 570nm to pass through, but reflect light
below 530nm and above 570nm.
9. Longpass Filter - ANSWER ✓ wavelength above 650nM
10. Shortpass Filter - ANSWER ✓ wavelength below 488nM
11. dichroics mirrors - ANSWER ✓ Dichroic mirrors can block light by phased
reflection allowing certain light to pass through and interfering with other
wavelengths. For example, a 500LP dichroic mirror would transmit light
above 500 nm and reflect the light below 500 nm in a different direction. A
525SP dichroic mirror would transmit all light below 525 nm and reflect all
light above 525 nm in a different direction. These dichroic mirrors are critical
in the directing and capturing of light by the detectors.
12. neutral density filter - ANSWER ✓ filter that reduces or modifies the intensity
of all wavelengths, or colors, of light equally, giving no changes in hue of color
rendition
, 13. polarization filter - ANSWER ✓ Polarization of scatter and fluorescence
signals in flow cytometry. ... depending on the light source(s), the optical
layout, and the types of mirrors and filters used.
14. light source - ANSWER ✓ The light source can be a laser, an arc lamp or even
an LED. Today, the majority of instruments use a laser. Lasers illuminate the
stream with coherent, focused light of specific wavelength (energy) and power.
This illumination facilitates the generation of fluorescence signals from cells
labeled with fluorophores and light scatter signals from redirected laser light.
15. arc lamp laser - ANSWER ✓ Arc lamps need optical filters to select the
appropriate wavelength. They do not give the sensitivity needed to observe
weak fluorescence but offer a cheaper alternative for observing strong
fluorescences, for example, in DNA analysis.
16. argon laser - ANSWER ✓ Air-cooled argon-ion laser producing blue light at
488 nm. This wavelength is convenient for the excitation of fluorescein, the
first immunofluorescent label to be used. Other air-cooled lasers in general
use include He-Ne (633 nm) and He-CD (325 nm).
17. solid state lasers - ANSWER ✓ Solid state lasers producing light at 355, 405,
488, 530, 594, 635 and 780 nm are available. Most solid state lasers produce
between 10 and 25 mW. There is at least one diode laser giving 200 mW at
488 nm.
WITH ACCURATE QUESTIONS AND
ANSWERS (VERIFIED ANSWERS) |
LATEST (2026/2027) UPDATED
VERSION | 100% GUARANTEED PASS
{JUST RELEASED}
1. Hydrodynamic Focusing - ANSWER ✓ Most modern flow cytometers tightly
position the sample for optical analysis via hydrodynamic focusing. Here, a
carrier fluid called the sheath fluid is used to position the sample of cells into a
single file for optical interrogation.
2. Hydordynamic focusing and sheath fluids - ANSWER ✓ The central stream
(sample stream) is focused and surrounded by the secondary slower stream
(sheath fluid). The shape and size of the flow cell is crucial to hydrodynamic
focusing, and traditionally the cell is nozzle shaped. ... In a flow cytometer, the
sheath fluid pressure is constant while the sample fluid is adjusted
3. Sample Pressure and the Sheath Pressure - ANSWER ✓ The difference
between the sample pressure and the sheath pressure is the differential
pressure. This controls the width of the core stream and the total number of
cells passing the laser intercept.
,4. differential pressure based flow cytometers - ANSWER ✓ Differential pressure
based flow cytometers currently dominate the market. These systems have two
pressure regulators. The first is at a constant pressure that sets how fast the
fluids runs at. The second is regulated by the investigator (like on this LSR-II
control panel).
5. Generation of differential pressure (syringe pump, pressure based) - ANSWER
✓ Low differential pressure allows the cells to move past the interrogation
point one at a time. .... One kind involves generating pressure using a pump
and regulator system ... Differential pressure based fluidic system. ...
peristaltic and/or syringe pumps to deliver the sample into the instrument.
6. Characterization of syringe-pump-driven induced pressure - ANSWER ✓ In
syringe-pump-driven microfluidic systems, pressure fluctuations are observed
in an elastic microchannel. The syringe pump is driven by an electrical stepper
motor, from which mechanical oscillations are expected to generate flow-rate
fluctuations and in turn leads to the pressure fluctuations in the channel flow.
7. Optical Filters - ANSWER ✓ Filters are pieces of glass coated on both sides
that allow light of a certain collection, or band, of wavelengths to pass through
while absorbing or interfering with photons of other wavelengths. These come
in bandpass, longpass, and shortpass flavors
, 8. Band Pass Optical Filter - ANSWER ✓ A filter that allows light between a set
wavelength to pass through and reflects light above and below the set
wavelength. For example, a bandpass filter with a wavelength of 550/40nm
would allow light between 530nm and 570nm to pass through, but reflect light
below 530nm and above 570nm.
9. Longpass Filter - ANSWER ✓ wavelength above 650nM
10. Shortpass Filter - ANSWER ✓ wavelength below 488nM
11. dichroics mirrors - ANSWER ✓ Dichroic mirrors can block light by phased
reflection allowing certain light to pass through and interfering with other
wavelengths. For example, a 500LP dichroic mirror would transmit light
above 500 nm and reflect the light below 500 nm in a different direction. A
525SP dichroic mirror would transmit all light below 525 nm and reflect all
light above 525 nm in a different direction. These dichroic mirrors are critical
in the directing and capturing of light by the detectors.
12. neutral density filter - ANSWER ✓ filter that reduces or modifies the intensity
of all wavelengths, or colors, of light equally, giving no changes in hue of color
rendition
, 13. polarization filter - ANSWER ✓ Polarization of scatter and fluorescence
signals in flow cytometry. ... depending on the light source(s), the optical
layout, and the types of mirrors and filters used.
14. light source - ANSWER ✓ The light source can be a laser, an arc lamp or even
an LED. Today, the majority of instruments use a laser. Lasers illuminate the
stream with coherent, focused light of specific wavelength (energy) and power.
This illumination facilitates the generation of fluorescence signals from cells
labeled with fluorophores and light scatter signals from redirected laser light.
15. arc lamp laser - ANSWER ✓ Arc lamps need optical filters to select the
appropriate wavelength. They do not give the sensitivity needed to observe
weak fluorescence but offer a cheaper alternative for observing strong
fluorescences, for example, in DNA analysis.
16. argon laser - ANSWER ✓ Air-cooled argon-ion laser producing blue light at
488 nm. This wavelength is convenient for the excitation of fluorescein, the
first immunofluorescent label to be used. Other air-cooled lasers in general
use include He-Ne (633 nm) and He-CD (325 nm).
17. solid state lasers - ANSWER ✓ Solid state lasers producing light at 355, 405,
488, 530, 594, 635 and 780 nm are available. Most solid state lasers produce
between 10 and 25 mW. There is at least one diode laser giving 200 mW at
488 nm.
