OpenStax Microbiology Test Bank
Chapter 13: Control of Microbial Growth
Chapter 13: Control of Microbial Growth
* = Correct answer
Multiple Choice
1.Which of the following is not an example of a fomite?
A.catheter
B.doorknob
C.mosquitoes*
D.towels
Difficulty: Easy
ASM Standard: 13, 14, 23
2.HEPA filters are required for which Biological Safety Level (BSL)?
A.BSL 1
B.BSL 2
C.BSL 3
D.BSL 4*
Difficulty: Easy
ASM Standard: 14, 37
3.From a clinical perspective, aseptic technique is carried out to do which of the following?
A.clean fomites
B.contaminate fomites
C.introduce contamination in living tissue
D.prevent contamination in living tissue*
Difficulty: Easy
ASM Standard: 36, 37
4.The autoclave uses which of the following mechanism(s) for sterilization? A.pressure
B.temperature
C.pressure and temperature*
D.pressure, temperature, and pH
Difficulty: Easy
ASM Standard: 14, 36, 37
5.Autoclaves are designed to kill which of the following heat-resistant microbes?
A.endospores*
B.prions
C.vegetative bacterial and fungal cells
Page 1 of 17 OpenStax Microbiology Test Bank
Chapter 13: Control of Microbial Growth
D.viruses
Difficulty: Easy
ASM Standard: 14, 36, 37
6.The decimal reduction time (D-value) is how long it takes to kill which percentage of a microbial population? A.10%
B.50%
C.90%*
D.100%
Difficulty: Moderate
ASM Standard: 14
7.Which of the following actions would be most clearly considered bacteriostatic rather than bactericidal?
A.autoclaving bacterial cultures
B.placing a bacterial culture in the −80 C freezer*
C.spraying 70% alcohol on a laboratory bench
D.treating a mattress with ethylene oxide
Difficulty: Moderate
ASM Standard: 14
8.Microbial death follows which type of trend?
A.linear
B.logarithmic*
C.power series
D.variable
Difficulty: Moderate
ASM Standard: 14
9.Which of the following are the standard conditions for an autoclave?
A.100 C, 1 psi, 60 minutes
B.121 C, 1 psi, 60 minutes
C.121 C, 15 psi, 20 minutes*
D.212 C, 15 psi, 10 minutes
Difficulty: Moderate
ASM Standard: 14, 36, 37
10.Which of the following is not a milkborne pathogen?
A.Escherichia coli O157:H7
B.Listeria monocytogenes
Page 2 of 17 OpenStax Microbiology Test Bank
Chapter 13: Control of Microbial Growth
C.Salmonella enterica
D.Yersinia pestis*
Difficulty: Moderate
ASM Standard: 23
11.For appropriate food storage in refrigerators, the temperature should be set to no higher than which of the following?
A.5 C
B.7 C*
C.9 C
D.11 C
Difficulty: Easy
ASM Standard: 14
12.How does pascalization control microbial growth?
A.by cross-linking proteins B.by damaging DNA
C.by denaturing proteins*
D.by disrupting membrane integrity
Difficulty: Moderate
ASM Standard: 14
13.X-rays and gamma rays are examples of which type of radiation?
A.beta emission radiation
B.ionizing radiation*
C.nonionizing radiation
D.nonpenetrating radiation
Difficulty: Easy
ASM Standard: 14
14.Ultraviolet light is an example of which type of radiation?
A.beta emission radiation
B.ionizing radiation
C.nonionizing radiation*
D.penetrating radiation
Difficulty: Easy
ASM Standard: 14
15.To sterilize packaged intravenous tubing, which method would not be acceptable?
A.ethylene oxide
B.freezing
Page 3 of 17
Chapter 13: Control of Microbial Growth
Chapter 13: Control of Microbial Growth
* = Correct answer
Multiple Choice
1.Which of the following is not an example of a fomite?
A.catheter
B.doorknob
C.mosquitoes*
D.towels
Difficulty: Easy
ASM Standard: 13, 14, 23
2.HEPA filters are required for which Biological Safety Level (BSL)?
A.BSL 1
B.BSL 2
C.BSL 3
D.BSL 4*
Difficulty: Easy
ASM Standard: 14, 37
3.From a clinical perspective, aseptic technique is carried out to do which of the following?
A.clean fomites
B.contaminate fomites
C.introduce contamination in living tissue
D.prevent contamination in living tissue*
Difficulty: Easy
ASM Standard: 36, 37
4.The autoclave uses which of the following mechanism(s) for sterilization? A.pressure
B.temperature
C.pressure and temperature*
D.pressure, temperature, and pH
Difficulty: Easy
ASM Standard: 14, 36, 37
5.Autoclaves are designed to kill which of the following heat-resistant microbes?
A.endospores*
B.prions
C.vegetative bacterial and fungal cells
Page 1 of 17 OpenStax Microbiology Test Bank
Chapter 13: Control of Microbial Growth
D.viruses
Difficulty: Easy
ASM Standard: 14, 36, 37
6.The decimal reduction time (D-value) is how long it takes to kill which percentage of a microbial population? A.10%
B.50%
C.90%*
D.100%
Difficulty: Moderate
ASM Standard: 14
7.Which of the following actions would be most clearly considered bacteriostatic rather than bactericidal?
A.autoclaving bacterial cultures
B.placing a bacterial culture in the −80 C freezer*
C.spraying 70% alcohol on a laboratory bench
D.treating a mattress with ethylene oxide
Difficulty: Moderate
ASM Standard: 14
8.Microbial death follows which type of trend?
A.linear
B.logarithmic*
C.power series
D.variable
Difficulty: Moderate
ASM Standard: 14
9.Which of the following are the standard conditions for an autoclave?
A.100 C, 1 psi, 60 minutes
B.121 C, 1 psi, 60 minutes
C.121 C, 15 psi, 20 minutes*
D.212 C, 15 psi, 10 minutes
Difficulty: Moderate
ASM Standard: 14, 36, 37
10.Which of the following is not a milkborne pathogen?
A.Escherichia coli O157:H7
B.Listeria monocytogenes
Page 2 of 17 OpenStax Microbiology Test Bank
Chapter 13: Control of Microbial Growth
C.Salmonella enterica
D.Yersinia pestis*
Difficulty: Moderate
ASM Standard: 23
11.For appropriate food storage in refrigerators, the temperature should be set to no higher than which of the following?
A.5 C
B.7 C*
C.9 C
D.11 C
Difficulty: Easy
ASM Standard: 14
12.How does pascalization control microbial growth?
A.by cross-linking proteins B.by damaging DNA
C.by denaturing proteins*
D.by disrupting membrane integrity
Difficulty: Moderate
ASM Standard: 14
13.X-rays and gamma rays are examples of which type of radiation?
A.beta emission radiation
B.ionizing radiation*
C.nonionizing radiation
D.nonpenetrating radiation
Difficulty: Easy
ASM Standard: 14
14.Ultraviolet light is an example of which type of radiation?
A.beta emission radiation
B.ionizing radiation
C.nonionizing radiation*
D.penetrating radiation
Difficulty: Easy
ASM Standard: 14
15.To sterilize packaged intravenous tubing, which method would not be acceptable?
A.ethylene oxide
B.freezing
Page 3 of 17