BIOC 384 EXAM 1 QUESTIONS WITH VERIFIED
ANSWERS
Select the TWO true statements below describing the standard free energy change
(delta G) and the equilibrium constant (Keq).
The equilibrium constant is equal to ratio of the concentration of reactants over products
at equilibrium.
If the value of the equilibrium constant is known, the value for the standard free energy
change can be determined.
The value for the standard free energy change is constant for a given reaction.
The standard free energy change is a function of enzyme concentration in the reaction.
A reaction is spontaneous when the standard free energy change and the equilibrium
constant are both negative values. - Answers - If the value of the equilibrium constant is
known, the value for the standard free energy change can be determined.
The value for the standard free energy change is constant for a given reaction.
Which of the of four amino acids residues listed in the choices below are all most likely
to be buried in the interior of a water soluble protein?
Ala, Asp, Cys, Lys
Glu, Thr, Phe, Arg
Glu, Trp, Tyr, His
Cys, Thr, Ser, Glu
Leu, Ile, Val, Ala - Answers - Leu, Ile, Val, Ala
G-C rich regions of DNA are more stable to denaturation than A-T rich regions. Why?
(choose one correct answer).
, The primary reason G-C rich regions are more stable is that G-C base pairs have three
hydrogen bonds, and A-T base pairs have only two hydrogen bonds.
Counterions (like Mg2+) bind preferentially to G-C rich regions, enhancing their stability.
More DNA binding proteins bind to G-C rich regions, and therefore stabilize these
structures.
G-C rich regions of DNA have more favorable base stacking energy than A-T rich
regions - Answers - G-C rich regions of DNA have more favorable base stacking energy
than A-T rich regions
Urea and β-mercaptoethanol (BME) were critical to the success of Anfinsen's protein
folding experiments using the enzyme ribonuclease. What was the most important
conclusion resulting from this experiment that earned Anfinsen the Nobel Prize?
That ribonuclease can form four disulfide bonds that function as "staples" holding the
protein together.
That BME is strong reductant that can permit protein folding to occur following a precise
pathway.
That the hydrophobic collapse model is the best explanation for protein folding in an
aqueous solution.
That primary structure of a protein has all the information necessary to specify tertiary
structure. - Answers - That primary structure of a protein has all the information
necessary to specify tertiary structure.
A hydrogen bond can best be described as a
weak noncovalent interaction.
strong covalent interaction.
weak covalent interaction.
weak hydrophobic effect.
strong ionic interaction. - Answers - weak noncovalent interaction.
ANSWERS
Select the TWO true statements below describing the standard free energy change
(delta G) and the equilibrium constant (Keq).
The equilibrium constant is equal to ratio of the concentration of reactants over products
at equilibrium.
If the value of the equilibrium constant is known, the value for the standard free energy
change can be determined.
The value for the standard free energy change is constant for a given reaction.
The standard free energy change is a function of enzyme concentration in the reaction.
A reaction is spontaneous when the standard free energy change and the equilibrium
constant are both negative values. - Answers - If the value of the equilibrium constant is
known, the value for the standard free energy change can be determined.
The value for the standard free energy change is constant for a given reaction.
Which of the of four amino acids residues listed in the choices below are all most likely
to be buried in the interior of a water soluble protein?
Ala, Asp, Cys, Lys
Glu, Thr, Phe, Arg
Glu, Trp, Tyr, His
Cys, Thr, Ser, Glu
Leu, Ile, Val, Ala - Answers - Leu, Ile, Val, Ala
G-C rich regions of DNA are more stable to denaturation than A-T rich regions. Why?
(choose one correct answer).
, The primary reason G-C rich regions are more stable is that G-C base pairs have three
hydrogen bonds, and A-T base pairs have only two hydrogen bonds.
Counterions (like Mg2+) bind preferentially to G-C rich regions, enhancing their stability.
More DNA binding proteins bind to G-C rich regions, and therefore stabilize these
structures.
G-C rich regions of DNA have more favorable base stacking energy than A-T rich
regions - Answers - G-C rich regions of DNA have more favorable base stacking energy
than A-T rich regions
Urea and β-mercaptoethanol (BME) were critical to the success of Anfinsen's protein
folding experiments using the enzyme ribonuclease. What was the most important
conclusion resulting from this experiment that earned Anfinsen the Nobel Prize?
That ribonuclease can form four disulfide bonds that function as "staples" holding the
protein together.
That BME is strong reductant that can permit protein folding to occur following a precise
pathway.
That the hydrophobic collapse model is the best explanation for protein folding in an
aqueous solution.
That primary structure of a protein has all the information necessary to specify tertiary
structure. - Answers - That primary structure of a protein has all the information
necessary to specify tertiary structure.
A hydrogen bond can best be described as a
weak noncovalent interaction.
strong covalent interaction.
weak covalent interaction.
weak hydrophobic effect.
strong ionic interaction. - Answers - weak noncovalent interaction.
