MCB LAB REVIEW UPDATED ACTUAL Questions and CORRECT
Answers
Question: Crystal Violet---T/F
Correct Answer: When working with cell lines, the number of cells is irrelevant because assays are cell
number independent?: FALSE
Question: Crystal Violet---T/F
Correct Answer: Media will serve as negative control: TRUE
Question: Crystal Violet----T/F
Correct Answer: We need a vehicle control to make sure that the vehicle is not affecting the cells, but only
the compound.: TRUE
Question: Crystal Violet---T/F
Correct Answer: If the wells have a purple color after adding the crystal violet it will suggest that there is
more cell survival?: TRUE
Question: Crystal Violet--If you have a solution of drug X at a concentration of 64uM. If your goal is to
make a 100ul stock at a final concentration of 16uM, how much volume of your initial stock do you need?
(use formula
Correct Answer: C1V1=C2V2): 25ul
Question: Crystal Violet
Correct Answer: Is this lab a quantitative or qualitative assessment?: Qualitative
Question: Crystal Violet
Correct Answer: ---Vehicle, positive, and negative controls are used throughout our experiments to serve
as what purpose for this lab?: Controls are necessary because they serve as reference points to guarantee the
changes that are noted are from the variables being introduced and nothing else.
Question: CRYSTAL VIOLET---What are the proper conditions to incubate cells?
Correct Answer: 37C & 5% CO2
Question: CRYSTAL VIOLET---Why is it important to remove the medium one row at a time?
Correct Answer: To prevent the cells from drying out
Question: CRYSTAL VIOLET---What does EC50 stand for?
Correct Answer: Effective Concentration at which 50% of cell/population is affected.
Question: Cell migration-----Select the series of events that happen after a tissue is wounded to repair the
damage
Correct Answer: : Increase vascularization by angiogenic factors. An increase of cell proliferation and
extracellular matrix deposition Infiltration by inflammatory immune cells as part of the process to destroy
necrotic tissue. Answers: Increase vascularization by angiogenic factors.
Question: Cell migration-----What is a metastatic disease?
Correct Answer: Is the spread of a disease from one organ (or part) to another non-adjacent organ.
Answers
Question: Crystal Violet---T/F
Correct Answer: When working with cell lines, the number of cells is irrelevant because assays are cell
number independent?: FALSE
Question: Crystal Violet---T/F
Correct Answer: Media will serve as negative control: TRUE
Question: Crystal Violet----T/F
Correct Answer: We need a vehicle control to make sure that the vehicle is not affecting the cells, but only
the compound.: TRUE
Question: Crystal Violet---T/F
Correct Answer: If the wells have a purple color after adding the crystal violet it will suggest that there is
more cell survival?: TRUE
Question: Crystal Violet--If you have a solution of drug X at a concentration of 64uM. If your goal is to
make a 100ul stock at a final concentration of 16uM, how much volume of your initial stock do you need?
(use formula
Correct Answer: C1V1=C2V2): 25ul
Question: Crystal Violet
Correct Answer: Is this lab a quantitative or qualitative assessment?: Qualitative
Question: Crystal Violet
Correct Answer: ---Vehicle, positive, and negative controls are used throughout our experiments to serve
as what purpose for this lab?: Controls are necessary because they serve as reference points to guarantee the
changes that are noted are from the variables being introduced and nothing else.
Question: CRYSTAL VIOLET---What are the proper conditions to incubate cells?
Correct Answer: 37C & 5% CO2
Question: CRYSTAL VIOLET---Why is it important to remove the medium one row at a time?
Correct Answer: To prevent the cells from drying out
Question: CRYSTAL VIOLET---What does EC50 stand for?
Correct Answer: Effective Concentration at which 50% of cell/population is affected.
Question: Cell migration-----Select the series of events that happen after a tissue is wounded to repair the
damage
Correct Answer: : Increase vascularization by angiogenic factors. An increase of cell proliferation and
extracellular matrix deposition Infiltration by inflammatory immune cells as part of the process to destroy
necrotic tissue. Answers: Increase vascularization by angiogenic factors.
Question: Cell migration-----What is a metastatic disease?
Correct Answer: Is the spread of a disease from one organ (or part) to another non-adjacent organ.
