BIOL 230 FINAL Questions and Answers with Verified Solutions |
Latest 2026 Update
Q: restriction endonucleases
Answer:
Enzymes that cut DNA at specific sequences.
Q: vectors
Answer:
bacteria or yeast plasmids derived from microorganisms and genetically
manipulated
Q: contain an origin of replication, the fragment of interest, and at least one gene
for antibiotic
Answer:
resistance
Q: making bacteria competent
Answer:
making it capable of taking up DNA
, BIOL 230 FINAL Questions and Answers with Verified Solutions |
Latest 2026 Update
Q: chemical competence - CaCl and heat shock
Answer:
Q: Electroporation - electric shock
Answer:
Q: steps in replication in vitro - PCR
Answer:
1. denaturation at high temperatures to separate strands
of DNA DENATURATION
Q: 2. temp drops to allow annealing of single strand DNA primers to DNA to
determine the
Answer:
fragment copied - recombine DNA ANNEALING
, BIOL 230 FINAL Questions and Answers with Verified Solutions |
Latest 2026 Update
Q: 3. at 37 degrees celsius, DNA polymerase will add nucleotides to the 3' end of
the annealed
Answer:
primers to extend the complementary strand EXTENSION
Q: Kary Mullis
Answer:
came up w/ pcr solution
Q: enzyme that can handle high temps
Answer:
Q: Taq DNA polymerase
Answer:
, BIOL 230 FINAL Questions and Answers with Verified Solutions |
Latest 2026 Update
Q: PCR issue
Answer:
every time replication occurred, the high temp for denaturing DNA would also
denature the polymerase, so fresh would need to be added every time
Q: Bacillus anthracis
Answer:
causes anthrax
Q: gram positive
Answer:
Q: forms endospores
Answer:
Latest 2026 Update
Q: restriction endonucleases
Answer:
Enzymes that cut DNA at specific sequences.
Q: vectors
Answer:
bacteria or yeast plasmids derived from microorganisms and genetically
manipulated
Q: contain an origin of replication, the fragment of interest, and at least one gene
for antibiotic
Answer:
resistance
Q: making bacteria competent
Answer:
making it capable of taking up DNA
, BIOL 230 FINAL Questions and Answers with Verified Solutions |
Latest 2026 Update
Q: chemical competence - CaCl and heat shock
Answer:
Q: Electroporation - electric shock
Answer:
Q: steps in replication in vitro - PCR
Answer:
1. denaturation at high temperatures to separate strands
of DNA DENATURATION
Q: 2. temp drops to allow annealing of single strand DNA primers to DNA to
determine the
Answer:
fragment copied - recombine DNA ANNEALING
, BIOL 230 FINAL Questions and Answers with Verified Solutions |
Latest 2026 Update
Q: 3. at 37 degrees celsius, DNA polymerase will add nucleotides to the 3' end of
the annealed
Answer:
primers to extend the complementary strand EXTENSION
Q: Kary Mullis
Answer:
came up w/ pcr solution
Q: enzyme that can handle high temps
Answer:
Q: Taq DNA polymerase
Answer:
, BIOL 230 FINAL Questions and Answers with Verified Solutions |
Latest 2026 Update
Q: PCR issue
Answer:
every time replication occurred, the high temp for denaturing DNA would also
denature the polymerase, so fresh would need to be added every time
Q: Bacillus anthracis
Answer:
causes anthrax
Q: gram positive
Answer:
Q: forms endospores
Answer:
