LAT CERTIFICATION EXAM
2026
Erasistratus -ANSWER✅✅✅✅Early philosopher that studied animal anatomy and was
the first to demonstrate that the heart's valves limit blood flow to one direction
Early 1500's -ANSWER✅✅✅✅The time period signifying the start of the modern
experimental era
William Harvey -ANSWER✅✅✅✅English physician and scientist who described the
circulation of the blood in the 1600s
Carl Linnaeus -ANSWER✅✅✅✅Physician and zoologist who developed a taxonomic
system that classified organisms based on anatomical traits
Claude Bernard -ANSWER✅✅✅✅Validated the scientific method in France (1800s)
Louis Pasteur -ANSWER✅✅✅✅A French chemist, this man discovered that heat
could kill bacteria that otherwise spoiled liquids. Studied infectious disease.
Robert Koch -ANSWER✅✅✅✅Developed the culture plate method to identify
pathogens
Dr. Walter Reed -ANSWER✅✅✅✅Confirmed that yellow fever was transmitted via
mosquito species in the early 1900s
Compound microscope -ANSWER✅✅✅✅Invented in the late 1500s, aided in research
development
Research study -ANSWER✅✅✅✅Systematic investigation to establish facts; Define
question --> formulate objective framework --> establish hypothesis --> create research
design --> identify variables --> collect/analyze data --> report data and conclusions
Grants -ANSWER✅✅✅✅Provide money for 3 to 5-year period during which the
investigator must conduct the study and publish results in one or more scientific journals
NIH -ANSWER✅✅✅✅Most ______ grants go to research programs at colleges and
universities
,NIH & NSF -ANSWER✅✅✅✅what are the 2 main public agencies that fund research
NSF -ANSWER✅✅✅✅Agency supporting non-biomedical research that may involved
animals (physics, chemistry, engineering, etc.)
Office of Research and Grants -ANSWER✅✅✅✅Administrative unit that manages
grants, supports, research activities, assists grantees in managing their grants, helps
search for grant opportunities, and protects intellectual property rights
OLAW (Office of Laboratory Animal Welfare) -ANSWER✅✅✅✅monitors compliance
with PHS policy by assured institutions through site visits
IACUC -ANSWER✅✅✅✅Mandated by federal law and institutional policies that
govern the use of animals in research
CEO -ANSWER✅✅✅✅Appoints members of the IACUC
USDA, OLAW, and AAALAC -ANSWER✅✅✅✅The IACUC can suspend animal-
related activities at any time, but they must notify _________
5 consecutive hours -ANSWER✅✅✅✅the twenty-eight hour law dictates that animals
cannot be transported for >28 hours without being unloaded for at least _______ for
rest, water, and food
Cruelty to Animals Act (Britain 1876) -ANSWER✅✅✅✅first law limiting animal
experimentation
AWR (Animal Welfare Regulations) -ANSWER✅✅✅✅mandate that protocols for
USDA-covered species may only be approved for 1 year
Laboratory Animal Welfare Act of 1966 -ANSWER✅✅✅✅first law in U.S. addressing
animal use in research; established care and use standards for animals including
housing, sale, transport, dealer licensing, facility registration, and identification of dogs
and cats
USDA -ANSWER✅✅✅✅tasked with enforcing the laboratory animal welfare act of
1966
Animal Welfare Act (AWA) -ANSWER✅✅✅✅the laboratory animal welfare act of 1966
was renamed to this in 1970 to cover all warm-blooded animals used in research, sold
as pets, or used in exhibitions; required all animal dealers to be licensed and mandated
use of pain-relieving drugs for animals used in regulated activities
,1985 amendment to the animal welfare act (AWA) -ANSWER✅✅✅✅called for
excersize for dogs and psychological enrichment for primates, minimizing pain/distress
and only 1 major surgery/animal with exceptions
National Agricultural Library (NAL) -ANSWER✅✅✅✅assists researchers in preventing
unnecessary duplication, reducing pain and distress (resulted from 1985 amendment to
AWA)
live or dead dog, cat, primate guinea pig, hamster, rabbit, or other warm-blooded
animals used for research/pets; excludes birds, rats, mice, farm animals, and horses
(not used for research) -ANSWER✅✅✅✅AWA definition for 'animal'
Animal Welfare Regulations -ANSWER✅✅✅✅developed by the USDA to enforce the
AWA; establishes rules and standards for humane care and treatment of covered
species
Terms, Regulations, Standards, Rules of Practice -ANSWER✅✅✅✅Four parts of the
animal welfare regulations (AWR) developed by the USDA to enforce the AWA
Animal and Plant Health Inspection Service (APHIS) -ANSWER✅✅✅✅administers
AWR (animal welfare regulations) to enforce the AWA (animal welfare act)
USDA veterinary medical officers (VMO) -ANSWER✅✅✅✅conduct unannounced
inspections of animal care and use programs following the Animal Care Inspection
Guide in order to enforce the AWA through APHIS
APHIS -ANSWER✅✅✅✅Animal and Plant Health Inspection Service
IO -ANSWER✅✅✅✅person responsible for making sure the organization is AWA-
compliant
USDA -ANSWER✅✅✅✅research facilities must register with ______ if they use AWA-
covered species, registration must be signed by IO/CEO
Annually -ANSWER✅✅✅✅Institutions housing USDA-covered animals must submit a
report to the USDA including location of animal facilities, how many animals used, and
pain categories HOW OFTEN?
Animal facility -ANSWER✅✅✅✅building, room, area, enclosure, or vehicle used for
animal confinement, transport, maintenance, breeding, or experiments (includes
satellite facilities, containment areas outside facility, and designated areas where
animals are housed for >12 hours
, B & C -ANSWER✅✅✅✅In which pain category(ies) are animals considered to
experience no pain or distress?
C -ANSWER✅✅✅✅Animal restraint and blood collection from peripheral vein is an
example of which pain category?
D -ANSWER✅✅✅✅Animals undergoing anesthesia for surgery, receiving analgesics,
which may experience pain or distress despite use of appropriate drugs are an example
of which pain category?
E -ANSWER✅✅✅✅A study involving animals implanted with tumor cells, experiencing
tumor growth is an example of which pain category?
AWR (Animal Welfare Regulations) -ANSWER✅✅✅✅establishes licensing
requirements for vendors or commercial carriers supplying/transporting animals for
research institutions
USDA -ANSWER✅✅✅✅Dealers and auction operators must be licensed by _______
to sell animals (live or dead)
A -ANSWER✅✅✅✅Which class of animal dealers raise and sell purposed-bred
animals themselves?
B -ANSWER✅✅✅✅Which class of animal dealers purchase and resell animals (ex.
random-source dogs/cats from shelters, NHP imports)?
5 days -ANSWER✅✅✅✅Animals must be at a shelter for how long before potential
sale to a research facility?
Tag, collar, microchip, or tattoo -ANSWER✅✅✅✅Adult animals must be identified via
_____ at time of sale/transport/disposal/acquisition; these materials are kept for 1 year
after euthanasia for USDA-review
OLAW -ANSWER✅✅✅✅monitors compliance with PHS policy and expects
institutions to self-regulate
PHS policy & AWR (animal welfare regulations) -ANSWER✅✅✅✅require inspections
at least every 6 months
The Guide for the Care and Use of Laboratory Animals -ANSWER✅✅✅✅makes
recommendations regarding humane care and use of laboratory animals
The Guide for Laboratory Animal Facilities and Care -ANSWER✅✅✅✅The original
(1963) name for The Guide for the Care and Use of Laboratory Animals
2026
Erasistratus -ANSWER✅✅✅✅Early philosopher that studied animal anatomy and was
the first to demonstrate that the heart's valves limit blood flow to one direction
Early 1500's -ANSWER✅✅✅✅The time period signifying the start of the modern
experimental era
William Harvey -ANSWER✅✅✅✅English physician and scientist who described the
circulation of the blood in the 1600s
Carl Linnaeus -ANSWER✅✅✅✅Physician and zoologist who developed a taxonomic
system that classified organisms based on anatomical traits
Claude Bernard -ANSWER✅✅✅✅Validated the scientific method in France (1800s)
Louis Pasteur -ANSWER✅✅✅✅A French chemist, this man discovered that heat
could kill bacteria that otherwise spoiled liquids. Studied infectious disease.
Robert Koch -ANSWER✅✅✅✅Developed the culture plate method to identify
pathogens
Dr. Walter Reed -ANSWER✅✅✅✅Confirmed that yellow fever was transmitted via
mosquito species in the early 1900s
Compound microscope -ANSWER✅✅✅✅Invented in the late 1500s, aided in research
development
Research study -ANSWER✅✅✅✅Systematic investigation to establish facts; Define
question --> formulate objective framework --> establish hypothesis --> create research
design --> identify variables --> collect/analyze data --> report data and conclusions
Grants -ANSWER✅✅✅✅Provide money for 3 to 5-year period during which the
investigator must conduct the study and publish results in one or more scientific journals
NIH -ANSWER✅✅✅✅Most ______ grants go to research programs at colleges and
universities
,NIH & NSF -ANSWER✅✅✅✅what are the 2 main public agencies that fund research
NSF -ANSWER✅✅✅✅Agency supporting non-biomedical research that may involved
animals (physics, chemistry, engineering, etc.)
Office of Research and Grants -ANSWER✅✅✅✅Administrative unit that manages
grants, supports, research activities, assists grantees in managing their grants, helps
search for grant opportunities, and protects intellectual property rights
OLAW (Office of Laboratory Animal Welfare) -ANSWER✅✅✅✅monitors compliance
with PHS policy by assured institutions through site visits
IACUC -ANSWER✅✅✅✅Mandated by federal law and institutional policies that
govern the use of animals in research
CEO -ANSWER✅✅✅✅Appoints members of the IACUC
USDA, OLAW, and AAALAC -ANSWER✅✅✅✅The IACUC can suspend animal-
related activities at any time, but they must notify _________
5 consecutive hours -ANSWER✅✅✅✅the twenty-eight hour law dictates that animals
cannot be transported for >28 hours without being unloaded for at least _______ for
rest, water, and food
Cruelty to Animals Act (Britain 1876) -ANSWER✅✅✅✅first law limiting animal
experimentation
AWR (Animal Welfare Regulations) -ANSWER✅✅✅✅mandate that protocols for
USDA-covered species may only be approved for 1 year
Laboratory Animal Welfare Act of 1966 -ANSWER✅✅✅✅first law in U.S. addressing
animal use in research; established care and use standards for animals including
housing, sale, transport, dealer licensing, facility registration, and identification of dogs
and cats
USDA -ANSWER✅✅✅✅tasked with enforcing the laboratory animal welfare act of
1966
Animal Welfare Act (AWA) -ANSWER✅✅✅✅the laboratory animal welfare act of 1966
was renamed to this in 1970 to cover all warm-blooded animals used in research, sold
as pets, or used in exhibitions; required all animal dealers to be licensed and mandated
use of pain-relieving drugs for animals used in regulated activities
,1985 amendment to the animal welfare act (AWA) -ANSWER✅✅✅✅called for
excersize for dogs and psychological enrichment for primates, minimizing pain/distress
and only 1 major surgery/animal with exceptions
National Agricultural Library (NAL) -ANSWER✅✅✅✅assists researchers in preventing
unnecessary duplication, reducing pain and distress (resulted from 1985 amendment to
AWA)
live or dead dog, cat, primate guinea pig, hamster, rabbit, or other warm-blooded
animals used for research/pets; excludes birds, rats, mice, farm animals, and horses
(not used for research) -ANSWER✅✅✅✅AWA definition for 'animal'
Animal Welfare Regulations -ANSWER✅✅✅✅developed by the USDA to enforce the
AWA; establishes rules and standards for humane care and treatment of covered
species
Terms, Regulations, Standards, Rules of Practice -ANSWER✅✅✅✅Four parts of the
animal welfare regulations (AWR) developed by the USDA to enforce the AWA
Animal and Plant Health Inspection Service (APHIS) -ANSWER✅✅✅✅administers
AWR (animal welfare regulations) to enforce the AWA (animal welfare act)
USDA veterinary medical officers (VMO) -ANSWER✅✅✅✅conduct unannounced
inspections of animal care and use programs following the Animal Care Inspection
Guide in order to enforce the AWA through APHIS
APHIS -ANSWER✅✅✅✅Animal and Plant Health Inspection Service
IO -ANSWER✅✅✅✅person responsible for making sure the organization is AWA-
compliant
USDA -ANSWER✅✅✅✅research facilities must register with ______ if they use AWA-
covered species, registration must be signed by IO/CEO
Annually -ANSWER✅✅✅✅Institutions housing USDA-covered animals must submit a
report to the USDA including location of animal facilities, how many animals used, and
pain categories HOW OFTEN?
Animal facility -ANSWER✅✅✅✅building, room, area, enclosure, or vehicle used for
animal confinement, transport, maintenance, breeding, or experiments (includes
satellite facilities, containment areas outside facility, and designated areas where
animals are housed for >12 hours
, B & C -ANSWER✅✅✅✅In which pain category(ies) are animals considered to
experience no pain or distress?
C -ANSWER✅✅✅✅Animal restraint and blood collection from peripheral vein is an
example of which pain category?
D -ANSWER✅✅✅✅Animals undergoing anesthesia for surgery, receiving analgesics,
which may experience pain or distress despite use of appropriate drugs are an example
of which pain category?
E -ANSWER✅✅✅✅A study involving animals implanted with tumor cells, experiencing
tumor growth is an example of which pain category?
AWR (Animal Welfare Regulations) -ANSWER✅✅✅✅establishes licensing
requirements for vendors or commercial carriers supplying/transporting animals for
research institutions
USDA -ANSWER✅✅✅✅Dealers and auction operators must be licensed by _______
to sell animals (live or dead)
A -ANSWER✅✅✅✅Which class of animal dealers raise and sell purposed-bred
animals themselves?
B -ANSWER✅✅✅✅Which class of animal dealers purchase and resell animals (ex.
random-source dogs/cats from shelters, NHP imports)?
5 days -ANSWER✅✅✅✅Animals must be at a shelter for how long before potential
sale to a research facility?
Tag, collar, microchip, or tattoo -ANSWER✅✅✅✅Adult animals must be identified via
_____ at time of sale/transport/disposal/acquisition; these materials are kept for 1 year
after euthanasia for USDA-review
OLAW -ANSWER✅✅✅✅monitors compliance with PHS policy and expects
institutions to self-regulate
PHS policy & AWR (animal welfare regulations) -ANSWER✅✅✅✅require inspections
at least every 6 months
The Guide for the Care and Use of Laboratory Animals -ANSWER✅✅✅✅makes
recommendations regarding humane care and use of laboratory animals
The Guide for Laboratory Animal Facilities and Care -ANSWER✅✅✅✅The original
(1963) name for The Guide for the Care and Use of Laboratory Animals
