ASCP MLT CHEMISTRY EXAM STUDY
GUIDE WITH ACCURATE QUESTIONS
AND ANSWERS (VERIFIED ANSWERS) |
LATEST (2026/2027) UPDATED
VERSION | 100% GUARANTEED PASS
{JUST RELEASED}
1. Which one of the following statements about acetaminophen
metabolism is false?
Choose the single best answer
A. It is metabolized in the kidney
B. It is largely metabolized in the liver to glucuronide and
sulfonate conjugates
C. A small amount is metabolized via cytochrome oxidase to a
reactive benzoquinoneimine intermediate
D. This intermediate becomes toxic to the liver once tissue
glutathione becomes depleted - ANSWER ✓ A;
, The benzoquinoneimine intermediate produced in the liver as a result
of hepatic cytochrome oxidase metabolism of acetaminophen is
responsible for hepatic necrosis via acylation of hepatic tissue. The
treatment of acetaminophen toxicity includes the use of N-
acetylcysteine (Mucomyst), which acts as a glutathione substitute.
The risk of hepatotoxicity is predicted with the Rumack-Matthew
nomogram, which uses the hours after ingestion and plasma
acetaminophen level to predict the probability of hepatotoxicity.
Severe hepatotoxicity can be fatal.
2. According to the 2010 ADA guidelines, HbA1C percent can be used as an
assay to diagnose diabetes.
True/False - ANSWER ✓ T;
The 2010 ADA Clinical Practice Recommendations added
measurement of HbA1C as a diagnostic assay for diagnosis of
diabetes.
3. Which of the following conditions would make a sample unsuitable for
lactic acid testing?
Choose the single best answer
A. Movement of hand or arm during specimen collection
, B. Received in the lab on ice
C. Centrifuged in a refrigerated centrifuge
D. Specimen centrifuged and plasma separated from cells less than
15 minutes after collection - ANSWER ✓ A;
Movement of the muscles in the hand or arm during specimen
collection will falsely increase lactic acid concentration. A blood
sample for lactic acid testing should be placed on ice immediately
after collection, centrifuged within 15 minutes of collection, and
plasma immediately separated from cells following centrifugation. If
possible, a refrigerated centrifuge should be used.
4. You are working in a clinical chemistry laboratory and are analyzing a
plasma glucose sample. The sample is flagged by the analyzer for being
"outside of linear range." You manually dilute the sample 1:2 and rerun
it. Again, you receive an "outside linear range" alert. You decide to
perform a different manual dilution. This time you manually dilute the
original sample again; this time using a 1:3 dilution. The instrument
gives you a glucose value of 150 mg/dL from this diluted sample. What is
the actual patient glucose result that you should report to the physician?
Choose the single best answer
A. 100 mg/dL
, B. 400 mg/dL
C. 600 mg/dL
D. 450 mg/dL
E. 1200 mg/dL - ANSWER ✓ D;
The correct response is option D: 450 mg/dl. A 1:3 dilution entails
mixing one part patient sample with two parts diluent, for a total
volume of 3 parts. In laboratory dilutions, the total volume is
considered a dilution factor. The dilution factor in this question is 3.
So a diluted sample with a value of 150 would need to be multiplied
by 3 to obtain the correct final answer. 150 x 3 = 450 mg/dl.
5. That portion of an enzyme which is separated from its cofactor is called
a(n):
Choose the single best answer
A. Partial enzyme
B. Isoenzyme
C. Coenzyme
D. Apoenzyme - ANSWER ✓ D;
GUIDE WITH ACCURATE QUESTIONS
AND ANSWERS (VERIFIED ANSWERS) |
LATEST (2026/2027) UPDATED
VERSION | 100% GUARANTEED PASS
{JUST RELEASED}
1. Which one of the following statements about acetaminophen
metabolism is false?
Choose the single best answer
A. It is metabolized in the kidney
B. It is largely metabolized in the liver to glucuronide and
sulfonate conjugates
C. A small amount is metabolized via cytochrome oxidase to a
reactive benzoquinoneimine intermediate
D. This intermediate becomes toxic to the liver once tissue
glutathione becomes depleted - ANSWER ✓ A;
, The benzoquinoneimine intermediate produced in the liver as a result
of hepatic cytochrome oxidase metabolism of acetaminophen is
responsible for hepatic necrosis via acylation of hepatic tissue. The
treatment of acetaminophen toxicity includes the use of N-
acetylcysteine (Mucomyst), which acts as a glutathione substitute.
The risk of hepatotoxicity is predicted with the Rumack-Matthew
nomogram, which uses the hours after ingestion and plasma
acetaminophen level to predict the probability of hepatotoxicity.
Severe hepatotoxicity can be fatal.
2. According to the 2010 ADA guidelines, HbA1C percent can be used as an
assay to diagnose diabetes.
True/False - ANSWER ✓ T;
The 2010 ADA Clinical Practice Recommendations added
measurement of HbA1C as a diagnostic assay for diagnosis of
diabetes.
3. Which of the following conditions would make a sample unsuitable for
lactic acid testing?
Choose the single best answer
A. Movement of hand or arm during specimen collection
, B. Received in the lab on ice
C. Centrifuged in a refrigerated centrifuge
D. Specimen centrifuged and plasma separated from cells less than
15 minutes after collection - ANSWER ✓ A;
Movement of the muscles in the hand or arm during specimen
collection will falsely increase lactic acid concentration. A blood
sample for lactic acid testing should be placed on ice immediately
after collection, centrifuged within 15 minutes of collection, and
plasma immediately separated from cells following centrifugation. If
possible, a refrigerated centrifuge should be used.
4. You are working in a clinical chemistry laboratory and are analyzing a
plasma glucose sample. The sample is flagged by the analyzer for being
"outside of linear range." You manually dilute the sample 1:2 and rerun
it. Again, you receive an "outside linear range" alert. You decide to
perform a different manual dilution. This time you manually dilute the
original sample again; this time using a 1:3 dilution. The instrument
gives you a glucose value of 150 mg/dL from this diluted sample. What is
the actual patient glucose result that you should report to the physician?
Choose the single best answer
A. 100 mg/dL
, B. 400 mg/dL
C. 600 mg/dL
D. 450 mg/dL
E. 1200 mg/dL - ANSWER ✓ D;
The correct response is option D: 450 mg/dl. A 1:3 dilution entails
mixing one part patient sample with two parts diluent, for a total
volume of 3 parts. In laboratory dilutions, the total volume is
considered a dilution factor. The dilution factor in this question is 3.
So a diluted sample with a value of 150 would need to be multiplied
by 3 to obtain the correct final answer. 150 x 3 = 450 mg/dl.
5. That portion of an enzyme which is separated from its cofactor is called
a(n):
Choose the single best answer
A. Partial enzyme
B. Isoenzyme
C. Coenzyme
D. Apoenzyme - ANSWER ✓ D;
