MCB 250 Exam 3 UPDATED ACTUAL
QUESTIONS AND CORRECT ANSWERS
The primary mechanism used to repair double-stranded DNA breaks is?
-nucleotide excision repair
-mismatch excision repair
-homologous recombination
-crossover during meiosis
-arm-linking - CORRECT ANSWER homologous recombination
During homologous recombination, strand invasion involves all of the following except?
-a double stranded break in the DNA
-two nearly identical DNA sequences
-alignment of DNA on different chromosomes
-formation of a heteroduplex sequence
-excision of abnormal bases - CORRECT ANSWER excision of abnormal bases
In homologous recombination, the term "patch product" refers to?
-the product of any Holiday junction resolution
-a region of heteroduplex flanked on either side by sequences that have not experienced
recombination
-short cDNAs in which the 2nd strand was generated with a random primer
-the junction of chromosomal crossing over
-a localized change in DNA resulting from DNA mismatch repair - CORRECT ANSWER a
region of heteroduplex flanked on either side by sequences that have no experienced recombination
In E.coli, which protein is central to the process of strand invasion?
-RecA
-RecD
-RecB
-RecC
, -RecUV - CORRECT ANSWER RecA
The prokaryotic protein Rho
-moves along ssRNA in a 5' to 3' direction towards the RNA-DNA duplex
-functions as a helices to separate the dsDNA
-pulls single stranded DNA out of the RNA pol active site
-binds RNA pol to the promoter region
-is critical for promoter clearance by RNA pol - CORRECT ANSWER moves along ssRNA in
a 5' to 3' direction towards the RNA-DNA duplex
Which of the following represents a major difference between transcription in bacteria and
eukaryotes?
-the need for a DNA primer to initiate RNA synthesis
-the requirement for DNA as a template for synthesis of mRNAs
-the cellular location at which mRNAs are synthesized
-the requirements for RNA pol
-the synthesis of Okazaki fragments to generate late strand transcripts - CORRECT ANSWER
the cellular location at which mRNAs are synthesized
During transcription in Eukaryotes, an important function of the pre-initiation complex is to?
-form an open transcription complex
-assemble the ribosome directly at the initiation site
-load the clamp onto the separated strands of the DNA
-bind a sigma factor to the promoter - CORRECT ANSWER form an open transcription factor
RNA splicing in eukaryotes...
-is completed when the U1, U2, U5, U6 snRNP complex removes remaining exons for degradation
-involves removal of introns from a gene sequence followed by transcription and subsequent splicing
of exons
-is typically characterized by a 5' GU splice junction and a 3' AG splice junction at the exon/intron
boundaries
QUESTIONS AND CORRECT ANSWERS
The primary mechanism used to repair double-stranded DNA breaks is?
-nucleotide excision repair
-mismatch excision repair
-homologous recombination
-crossover during meiosis
-arm-linking - CORRECT ANSWER homologous recombination
During homologous recombination, strand invasion involves all of the following except?
-a double stranded break in the DNA
-two nearly identical DNA sequences
-alignment of DNA on different chromosomes
-formation of a heteroduplex sequence
-excision of abnormal bases - CORRECT ANSWER excision of abnormal bases
In homologous recombination, the term "patch product" refers to?
-the product of any Holiday junction resolution
-a region of heteroduplex flanked on either side by sequences that have not experienced
recombination
-short cDNAs in which the 2nd strand was generated with a random primer
-the junction of chromosomal crossing over
-a localized change in DNA resulting from DNA mismatch repair - CORRECT ANSWER a
region of heteroduplex flanked on either side by sequences that have no experienced recombination
In E.coli, which protein is central to the process of strand invasion?
-RecA
-RecD
-RecB
-RecC
, -RecUV - CORRECT ANSWER RecA
The prokaryotic protein Rho
-moves along ssRNA in a 5' to 3' direction towards the RNA-DNA duplex
-functions as a helices to separate the dsDNA
-pulls single stranded DNA out of the RNA pol active site
-binds RNA pol to the promoter region
-is critical for promoter clearance by RNA pol - CORRECT ANSWER moves along ssRNA in
a 5' to 3' direction towards the RNA-DNA duplex
Which of the following represents a major difference between transcription in bacteria and
eukaryotes?
-the need for a DNA primer to initiate RNA synthesis
-the requirement for DNA as a template for synthesis of mRNAs
-the cellular location at which mRNAs are synthesized
-the requirements for RNA pol
-the synthesis of Okazaki fragments to generate late strand transcripts - CORRECT ANSWER
the cellular location at which mRNAs are synthesized
During transcription in Eukaryotes, an important function of the pre-initiation complex is to?
-form an open transcription complex
-assemble the ribosome directly at the initiation site
-load the clamp onto the separated strands of the DNA
-bind a sigma factor to the promoter - CORRECT ANSWER form an open transcription factor
RNA splicing in eukaryotes...
-is completed when the U1, U2, U5, U6 snRNP complex removes remaining exons for degradation
-involves removal of introns from a gene sequence followed by transcription and subsequent splicing
of exons
-is typically characterized by a 5' GU splice junction and a 3' AG splice junction at the exon/intron
boundaries
