ACS BIOCHEMISTRY TEST PAPER
COMPLETE QUESTIONS AND ACCURATE
ANSWERS PREMIUM REVIEW SHEET
●● thioester bond (1)
Answer: compounds with functional group C-S-CoA-C (eg, in acetyl-
CoA)
●● zwitterion (3)
Answer: dipolar ion with both positive and negative groups but overall
neutral charge; can act as either an acid or base
●● Isoelectric point (pI) (chapter 3)
Answer: the characteristic pH at which the net electric charge is zero
●● SDS - Sodium Dodecyl Sulfate (chap 3)
Answer: detergent used to unfold proteins and give them uniform
negative charge
●● SDS Page (3)
Answer: chromatography used to separate proteins based on mass. light
proteins travel fast than heavier ones
, ●● PCR - Polymerase chain reaction (3)
Answer: copies DNA multiple times to increase sample size
●● Isoelectric focusing (chapt 3)
Answer: procedure used to determine the isoelectric pt (pI) of a protein.
Protein migrates through gel until pH = pI (net charge = 0)
●● Two-Dimensional Electrophoresis (3)
Answer: combines isoelectric focusing and SDS electrophoresis;
separates proteins by both molecular weight and pI
●● specific activity (3)
Answer: number of enzyme units per mg of total protein (a measure of
enzyme purity)
●● activity (3)
Answer: total units of a certain enzyme in a solution
●● Peptide bonds (chapter 4)
Answer: C-N bond with double bond character due to resonance (C-N
bond cannot rotate, and is planar)
●● Edman degredation (3)
COMPLETE QUESTIONS AND ACCURATE
ANSWERS PREMIUM REVIEW SHEET
●● thioester bond (1)
Answer: compounds with functional group C-S-CoA-C (eg, in acetyl-
CoA)
●● zwitterion (3)
Answer: dipolar ion with both positive and negative groups but overall
neutral charge; can act as either an acid or base
●● Isoelectric point (pI) (chapter 3)
Answer: the characteristic pH at which the net electric charge is zero
●● SDS - Sodium Dodecyl Sulfate (chap 3)
Answer: detergent used to unfold proteins and give them uniform
negative charge
●● SDS Page (3)
Answer: chromatography used to separate proteins based on mass. light
proteins travel fast than heavier ones
, ●● PCR - Polymerase chain reaction (3)
Answer: copies DNA multiple times to increase sample size
●● Isoelectric focusing (chapt 3)
Answer: procedure used to determine the isoelectric pt (pI) of a protein.
Protein migrates through gel until pH = pI (net charge = 0)
●● Two-Dimensional Electrophoresis (3)
Answer: combines isoelectric focusing and SDS electrophoresis;
separates proteins by both molecular weight and pI
●● specific activity (3)
Answer: number of enzyme units per mg of total protein (a measure of
enzyme purity)
●● activity (3)
Answer: total units of a certain enzyme in a solution
●● Peptide bonds (chapter 4)
Answer: C-N bond with double bond character due to resonance (C-N
bond cannot rotate, and is planar)
●● Edman degredation (3)
