l1 MIMG C185A Problem Set 2: Detailed Solutions andl1 l1 l1 l1 l1 l1 l1
l1 Explanations | New Update | | Complete A+ l1 l1 l1 l1 l1 l1 l1 l1 l1 l1
l1 Guide
PROBLEM SET 2 l1 l1
Question 1 (100 points) l1 l1 l1
You are a student from an immunology lab. You perform three independent experiments
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using different mouse strains and you want to explain your results with what you have
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learned from MIMG 185.
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a) In the first set of experiments, you infect two strains of mice with extracellular bacteria.
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You test their serum cytokine levels and get the following results: +++ (strong response),
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++ or + (lower response), +/- (partial response), - (no response).
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Cytokines Strain 1 Mice Th2 l1 l1 l1 Strain 2 Mice Th1 l1 l1 l1 Normal level l1
+ Extracellular Bacteria
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IFN -/+ +++ +
IL-4 +++ -/+ +
IL-5 ++ - +
IL-10 ++ - +
TNF - ++ +
Briefly explain (2-3 sentences) what are the differences between strain 1 and strain 2 in
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response to the same infection. (15 points)
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Serum from strain 1 mice show higher than normal IL-4, IL-5 and IL-10 and lower than
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normal IFN and TNF. Serum from strain 2 mice have lower than normal IL-4, IL-5 and IL-10
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and higher than normal IFN and TNF. This suggests an imbalance in the Th1/Th2 response;
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strain 1 favors Th2 and strain 2 favors Th1.
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Which strain of mice will survive better? Explain your answer in no more than 3 sentences.
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(15 points)
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Strain 1, Th2 extracellular response (the question says extracellular bacteria so extracellular
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response will be good to survive)
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Th1 (intracellular response) activates cellular responses while Th2 activates B cells to produce
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antibodies.
l 1
,
l1 Explanations | New Update | | Complete A+ l1 l1 l1 l1 l1 l1 l1 l1 l1 l1
l1 Guide
PROBLEM SET 2 l1 l1
Question 1 (100 points) l1 l1 l1
You are a student from an immunology lab. You perform three independent experiments
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using different mouse strains and you want to explain your results with what you have
l1 l1 l1 l1 l1 l1 l1 l1 l1 l1 l1 l1 l1 l1 l1
learned from MIMG 185.
l1 l1 l1 l1
a) In the first set of experiments, you infect two strains of mice with extracellular bacteria.
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You test their serum cytokine levels and get the following results: +++ (strong response),
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++ or + (lower response), +/- (partial response), - (no response).
l1 l1 l1 l1 l1 l1 l1 l1 l1 l1 l1
Cytokines Strain 1 Mice Th2 l1 l1 l1 Strain 2 Mice Th1 l1 l1 l1 Normal level l1
+ Extracellular Bacteria
l1 l1 + Extracellular Bacteria
l1 l1 (uninfected)
l1
IFN -/+ +++ +
IL-4 +++ -/+ +
IL-5 ++ - +
IL-10 ++ - +
TNF - ++ +
Briefly explain (2-3 sentences) what are the differences between strain 1 and strain 2 in
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response to the same infection. (15 points)
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Serum from strain 1 mice show higher than normal IL-4, IL-5 and IL-10 and lower than
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normal IFN and TNF. Serum from strain 2 mice have lower than normal IL-4, IL-5 and IL-10
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and higher than normal IFN and TNF. This suggests an imbalance in the Th1/Th2 response;
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strain 1 favors Th2 and strain 2 favors Th1.
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Which strain of mice will survive better? Explain your answer in no more than 3 sentences.
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(15 points)
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Strain 1, Th2 extracellular response (the question says extracellular bacteria so extracellular
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response will be good to survive)
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Th1 (intracellular response) activates cellular responses while Th2 activates B cells to produce
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antibodies.
l 1
,
