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CG(ASCP) Comprehensive Resource To Help You Ace Exams Includes Frequently Tested Questions With ELABORATED 100% Correct COMPLETE SOLUTIONS Guaranteed Pass First Attempt!! Current Update!!

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CG(ASCP) Comprehensive Resource To Help You Ace Exams Includes Frequently Tested Questions With ELABORATED 100% Correct COMPLETE SOLUTIONS Guaranteed Pass First Attempt!! Current Update!! The most important reason why biological safety cabinets are used instead of horizontal laminar flow hoods in the handling of human tissues is = to protect the technologist who is processing the specimens In which of the following situations would one suspect bacterial contamination = culture media is turbid in appearance Which of the following chemicals is used for cryopreservation? =dimethyl sulfoxide (DMSD) Which of the following is a butter used in cultures? = HEPES In order for the fragile X to be expressed in affected individuals, lymphocytes must be grown in what type of media? = folic acid deficient The following enzymes cab be used to dissociate Chronic Villus samples (CVS) except for = RNAse Which of the following is not a necessary ingredient which is added to modified medium? = antibiotics All of the following are factors which affect the cryopreservation except A fast growing culture shows signs of cell death. What is a possible reason for this? = cell confluency: too many or too few cells Collagenase, pronase, and trypsin are used to disperse tissue into single-cell suspension. These substances are: = proteolytic enzymes When harvesting peripheral blood cultures, asceptic technique no longer matters following incubation with: = colcemid Which of the following culture processes would be most useful for identifying patients w/ fanconi anemia? = mitomycin-C stress test of peripheral blood To avoid potential culture failure, it is important to monitor the reagents used. Recording which of the following information will be most important when documenting culture failure? = expiration date

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CG(ASCP) Comprehensive Resource To Help You Ace 2026-2027 Exams Includes Frequently Tested
Questions With ELABORATED 100% Correct COMPLETE SOLUTIONS
Guaranteed Pass First Attempt!! Current Update!!


The most important reason why biological safety cabinets
to protect the technologist who is processing the speci-
are used instead of horizontal laminar flow hoods in the
mens
handling of human tissues is
In which of the following situations would one suspect
culture media is turbid in appearance
bacterial contamination
Which of the following chemicals is used for cryopreser-
dimethyl sulfoxide (DMSD)
vation?
Which of the following is a butter used in cultures? HEPES
In order for the fragile X to be expressed in attected
individuals, lymphocytes must be grown in what type of folic acid deficient
media?
The following enzymes cab be used to dissociate Chronic
RNAse
Villus samples (CVS) except for
Which of the following is not a necessary ingredient which
antibiotics
is added to modified medium?
All of the following are factors which attect the cryopreser-
vation except
A fast growing culture shows signs of cell death. What is a
cell confluency: too many or too few cells
possible reason for this?
collagenase, pronase, and trypsin are used to disperse
proteolytic enzymes
tissue into single-cell suspension. These substances are:
When harvesting peripheral blood cultures, asceptic tech-
colcemid
nique no longer matters following incubation with:
Which of the following culture processes would be most
mitomycin-C stress test of peripheral blood
useful for identifying patients w/ fanconi anemia?

To avoid potential culture failure, it is important to monitor
expiration date
the reagents used. Recording which of the following infor-


, CG(ASCP) Comprehensive Resource To Help You Ace 2026-2027 Exams Includes Frequently Tested
Questions With ELABORATED 100% Correct COMPLETE SOLUTIONS
Guaranteed Pass First Attempt!! Current Update!!


mation will be most important when documenting culture
failure?
The morning after being set-up, a single dish of amniot-
ic fluid culture turns cloudy and appears contaminated.
harvest the culture as soon as possible
None of the other cultures set up that day appear conta-
minated. Which of the following should be done first?
If an amniotic fluid culture from the same sample grows
well in a ditterent incubator, the probable cause of the incubator failure
culture failure is
Positive staining w/ Hoechst 33258 reveals micronuclei.
Which of the following agents caused the culture conta- mycoplasma
mination?
Which of the following statements is true about trypsin> is is useful in removing cells from culture flasks
Which of the following is the best tissue to use for both a
good success rate and abnormality detection in sponta- chorionic villi
neous abortion specimen
When a culture becomes contaminated, the culture medi-
um shifts to an acidic pH. If the medium contains a pH yellow
indicator, the color of the medium in the flask will appear
Which of the following reagents is used in culturing pe-
ripheral blood, but not in culturing bone marrow or am- phytohemagglutinin (PHA)
niotic fluid or chromosome analysis?
Which of the following is the best agent to use for the
BM cycline
presence of mycoplasma growth in a culture?
A bone marrow culture on a patient w/ APL M3 has failed
to yield analyzable metaphases. The culture had a cell
density of 1x10^4 calls/ml. The specimen had been in too much time in transit
transit for 2 days and was not sent in a transport medium.
The most likely cause of the failure is:


, CG(ASCP) Comprehensive Resource To Help You Ace 2026-2027 Exams Includes Frequently Tested
Questions With ELABORATED 100% Correct COMPLETE SOLUTIONS
Guaranteed Pass First Attempt!! Current Update!!


Which of the following microscopes should be used when
dissection
cleaning a chorionic villus specimen?
Blood specimen for chromosome analysis may be kept in
96 hours
culture up to how many hours after culture initiation?
which of the following cell types is diagnostically most
maternal decidua
problematic if present in a CVS sample?
Cells from a skin fibroblast culture are to be frozen for stor-
age in liquid nitrogen. The cell concentration is 2X10^7
5
cells/ml. How many 2ml vials can be frozen if the final cell
concentration for storage is to be 2X10^6?
The time that a flask culture takes to reach confluency
humidity of the culture environment
depends on all of the following except the
Which of the following is true regarding closed-system
there is less chance of microbial contamination
cultures?
A blood culture for a patient diagnosed w/ Di-George
that an insuflcient number of cells were seeded in the
syndrome fails to grow in culture. The most likely cause
culture
of the failure is:
To maximize the ettectiveness of the biological safety cab- move his or her arms in and out slowly, perpendicular to
inet, the technologist should the opening
The following solution is used to detach monolayer cells Trypsin- EDTA
Which of the following will help prevent microbial and
aseptic technique
cross contamination of cultures?
Which of the following factors are not important for chro-
mitotic index
mosome spreading?
A good banding pattern will be expected when we ob-
serve chromosomes before banding under the phase dark
scope with the following appearance:
Karyotype with a del 5q

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