ASCP CG UPDATED FINAL EXAM QUESTIONS
AND ANSWERS SURE A+
✔✔If a tech with a cold and sneezing does not take proper precautions while setting up
amniotic fluid samples, the specimen could become contaminated with: -
✔✔Mycoplasma
--> the most common contaminant by a sick tech and can be detected through a specific
stain used on the sample
mycoplasma can induce abnormalities in a sample, so it is important to avoid
contamination
✔✔What about the metaphases on a slide are affected by slide making? - ✔✔Cell
density - thru dilution of the sample with fix
Banding - based on how dark/pale/refractive they are affects trypsin time
Chromosome spreading - affected by drying time
✔✔What test(s) should be ordered on a newborn with suspected Prader-Willi
syndrome? - ✔✔High resolution banding and FISH for the Prader-Willi locus on 15q11.2
,✔✔When capturing a metaphase, a tech cannot get the met into focus, what is the most
likely cause? - ✔✔The camera is not parfocal (in focus with the microscpe objectives)
with the microscope
✔✔Unstained chromosome preparation should be viewed using which scope? -
✔✔Phase contrast condenser
✔✔The minimum band level necessary to detect prader-willi syndrome is? - ✔✔550 at
the lowest
15q11.2 is visible at that level
See CAP regulation CYG.42300
✔✔A regulatory inspecter requests a copy of a lab patient's record for his inspection
files, the tech should: - ✔✔Deny the inspector a copy of the record
See HIPAA compliance policy
✔✔According to CAP regulations, what is the minimum band level for consitutional
specimens? - ✔✔400
The suggested level for most constitutional specimen is 550, but 400 is the lowest
acceptible level
See CAP regulation CYG.42200
✔✔Placental tissue is submitted for chromosome analysis, to optimize the liklihood of
obtaining fetal cells, where should the tech select tissue from? - ✔✔The fetal side of the
spcimen (when possible to be identified from the decidua)
✔✔In a compound light microscope, the aperature stop affects resolution and contrast
by: - ✔✔Changing the angle of the cone of light inside the objective
✔✔What is the correct nomenclature to describe a hematologic disorder with trisomy 21
in 6 cells and 14 cells that are normal? - ✔✔47,XY,+21?c[6]/46,XY[14]
?c -- indicates questioning whether the trisomy is constitutional or not as it was seen in
only 6 cells
✔✔The G-light bands produced by routine GTG banding represent: - ✔✔The active
regions of the chromosome
aka euchromatin -- gene-rich and GC rich DNA, early replicating
✔✔Which of the following is not assocated with a microdeletion?
, 1. Prader-Willi
2. DiGeorge
3. Rubinstein-Taybi
4. Langer-Giedion - ✔✔3. Rubinstein-Taybi
Associated with an autosomal dominant mutation in the CREB or CREBBP gene(s)
Rarely assocated with a deletion in 16p13.3 (12% of cases, not the predominant cause,
detected by FISH)
Presents as facial dysmorphism, broad thumbs, broad big toes, and growth and mental
retardation
✔✔The dark bands produced by routune GTG banding represent: - ✔✔AT-rich regions
of the chromosome
aka heterpchromatin, and gene-poor chromatin, late replicating DNA
✔✔If GTG banded chromosomes appear ghost-like with little visible banding, one
should alter banding prep by: - ✔✔Decreasing trypsin time (chewed out and non-
distinct)
✔✔If 50 out of 200 interphase nuclei have 3 signals in a prenatal aneusomy FISH
analysis for chromosome 18, the results are: - ✔✔Consistent with trisomy 18 or a
structural anomaly of chromosome 18
✔✔If the same hybridization incubator is used for several different probe strategies, the
stringency for each probe can be adjusted by: - ✔✔adjusting the formamide and salt
concentrations
✔✔Optimal (best) banding quality is achieved for each patient sample by banding: -
✔✔one trial slide per patient to ensure BEST quality
✔✔In fluorescent microscopy, the exciter and barrier filters used depend on ___ ? -
✔✔Stain - because the wavelength for emission can vary per type of fluorescent stain
used (i.e. DAPI)
✔✔What is a possible reason a metaphase cannot be located when switching from the
40x lens to the 100x oil lens? - ✔✔The 100x objective is slightly unscrewed (can occur
when cleaning the scope)
✔✔What is an action a technologist can take to reduce the risk of repetitve motion
injuries when doing analysis on a computer? - ✔✔Supporting the arm that moves the
mouse,
AND ANSWERS SURE A+
✔✔If a tech with a cold and sneezing does not take proper precautions while setting up
amniotic fluid samples, the specimen could become contaminated with: -
✔✔Mycoplasma
--> the most common contaminant by a sick tech and can be detected through a specific
stain used on the sample
mycoplasma can induce abnormalities in a sample, so it is important to avoid
contamination
✔✔What about the metaphases on a slide are affected by slide making? - ✔✔Cell
density - thru dilution of the sample with fix
Banding - based on how dark/pale/refractive they are affects trypsin time
Chromosome spreading - affected by drying time
✔✔What test(s) should be ordered on a newborn with suspected Prader-Willi
syndrome? - ✔✔High resolution banding and FISH for the Prader-Willi locus on 15q11.2
,✔✔When capturing a metaphase, a tech cannot get the met into focus, what is the most
likely cause? - ✔✔The camera is not parfocal (in focus with the microscpe objectives)
with the microscope
✔✔Unstained chromosome preparation should be viewed using which scope? -
✔✔Phase contrast condenser
✔✔The minimum band level necessary to detect prader-willi syndrome is? - ✔✔550 at
the lowest
15q11.2 is visible at that level
See CAP regulation CYG.42300
✔✔A regulatory inspecter requests a copy of a lab patient's record for his inspection
files, the tech should: - ✔✔Deny the inspector a copy of the record
See HIPAA compliance policy
✔✔According to CAP regulations, what is the minimum band level for consitutional
specimens? - ✔✔400
The suggested level for most constitutional specimen is 550, but 400 is the lowest
acceptible level
See CAP regulation CYG.42200
✔✔Placental tissue is submitted for chromosome analysis, to optimize the liklihood of
obtaining fetal cells, where should the tech select tissue from? - ✔✔The fetal side of the
spcimen (when possible to be identified from the decidua)
✔✔In a compound light microscope, the aperature stop affects resolution and contrast
by: - ✔✔Changing the angle of the cone of light inside the objective
✔✔What is the correct nomenclature to describe a hematologic disorder with trisomy 21
in 6 cells and 14 cells that are normal? - ✔✔47,XY,+21?c[6]/46,XY[14]
?c -- indicates questioning whether the trisomy is constitutional or not as it was seen in
only 6 cells
✔✔The G-light bands produced by routine GTG banding represent: - ✔✔The active
regions of the chromosome
aka euchromatin -- gene-rich and GC rich DNA, early replicating
✔✔Which of the following is not assocated with a microdeletion?
, 1. Prader-Willi
2. DiGeorge
3. Rubinstein-Taybi
4. Langer-Giedion - ✔✔3. Rubinstein-Taybi
Associated with an autosomal dominant mutation in the CREB or CREBBP gene(s)
Rarely assocated with a deletion in 16p13.3 (12% of cases, not the predominant cause,
detected by FISH)
Presents as facial dysmorphism, broad thumbs, broad big toes, and growth and mental
retardation
✔✔The dark bands produced by routune GTG banding represent: - ✔✔AT-rich regions
of the chromosome
aka heterpchromatin, and gene-poor chromatin, late replicating DNA
✔✔If GTG banded chromosomes appear ghost-like with little visible banding, one
should alter banding prep by: - ✔✔Decreasing trypsin time (chewed out and non-
distinct)
✔✔If 50 out of 200 interphase nuclei have 3 signals in a prenatal aneusomy FISH
analysis for chromosome 18, the results are: - ✔✔Consistent with trisomy 18 or a
structural anomaly of chromosome 18
✔✔If the same hybridization incubator is used for several different probe strategies, the
stringency for each probe can be adjusted by: - ✔✔adjusting the formamide and salt
concentrations
✔✔Optimal (best) banding quality is achieved for each patient sample by banding: -
✔✔one trial slide per patient to ensure BEST quality
✔✔In fluorescent microscopy, the exciter and barrier filters used depend on ___ ? -
✔✔Stain - because the wavelength for emission can vary per type of fluorescent stain
used (i.e. DAPI)
✔✔What is a possible reason a metaphase cannot be located when switching from the
40x lens to the 100x oil lens? - ✔✔The 100x objective is slightly unscrewed (can occur
when cleaning the scope)
✔✔What is an action a technologist can take to reduce the risk of repetitve motion
injuries when doing analysis on a computer? - ✔✔Supporting the arm that moves the
mouse,
