ACS Biochemistry Exam Question and
Answer 2026 | Final Review and Exam
Preparation Pack | Grade A+
• thioester bond (1) -✓✓compounds with functional group C-S-CoA-C
(eg, in acetyl-CoA)
• zwitterion (3) -✓✓dipolar ion with both positive and negative groups
but overall neutral charge; can act as either an acid or base
• Isoelectric point (pI) (chapter 3) -✓✓the characteristic pH at which the
net electric charge is zero
• SDS - Sodium Dodecyl Sulfate (chap 3) -✓✓detergent used to unfold
proteins and give them uniform negative charge
• SDS Page (3) -✓✓chromatography used to separate proteins based on
mass. light proteins travel fast than heavier ones
• PCR - Polymerase chain reaction (3) -✓✓copies DNA multiple times
to increase sample size
• Isoelectric focusing (chapt 3) -✓✓procedure used to determine the
isoelectric pt (pI) of a protein. Protein migrates through gel until pH = pI
(net charge = 0)
, • Two-Dimensional Electrophoresis (3) -✓✓combines isoelectric
focusing and SDS electrophoresis; separates proteins by both molecular
weight and pI
• specific activity (3) -✓✓number of enzyme units per mg of total
protein (a measure of enzyme purity)
• activity (3) -✓✓total units of a certain enzyme in a solution
• Peptide bonds (chapter 4) -✓✓C-N bond with double bond character
due to resonance (C-N bond cannot rotate, and is planar)
• Edman degredation (3) -✓✓used in the sequencing of polypeptides;
labels and removes ONLY the amino-residue from a polypeptide.
carried out in a machine called a sequenator
• φ in peptide bonding (chapter 4) -✓✓angle around the α-carbon -
amide nitrogen bond
• ψ in peptide bonding (chapter 4) -✓✓angle around the α-carbon -
carbonyl carbon bond
Answer 2026 | Final Review and Exam
Preparation Pack | Grade A+
• thioester bond (1) -✓✓compounds with functional group C-S-CoA-C
(eg, in acetyl-CoA)
• zwitterion (3) -✓✓dipolar ion with both positive and negative groups
but overall neutral charge; can act as either an acid or base
• Isoelectric point (pI) (chapter 3) -✓✓the characteristic pH at which the
net electric charge is zero
• SDS - Sodium Dodecyl Sulfate (chap 3) -✓✓detergent used to unfold
proteins and give them uniform negative charge
• SDS Page (3) -✓✓chromatography used to separate proteins based on
mass. light proteins travel fast than heavier ones
• PCR - Polymerase chain reaction (3) -✓✓copies DNA multiple times
to increase sample size
• Isoelectric focusing (chapt 3) -✓✓procedure used to determine the
isoelectric pt (pI) of a protein. Protein migrates through gel until pH = pI
(net charge = 0)
, • Two-Dimensional Electrophoresis (3) -✓✓combines isoelectric
focusing and SDS electrophoresis; separates proteins by both molecular
weight and pI
• specific activity (3) -✓✓number of enzyme units per mg of total
protein (a measure of enzyme purity)
• activity (3) -✓✓total units of a certain enzyme in a solution
• Peptide bonds (chapter 4) -✓✓C-N bond with double bond character
due to resonance (C-N bond cannot rotate, and is planar)
• Edman degredation (3) -✓✓used in the sequencing of polypeptides;
labels and removes ONLY the amino-residue from a polypeptide.
carried out in a machine called a sequenator
• φ in peptide bonding (chapter 4) -✓✓angle around the α-carbon -
amide nitrogen bond
• ψ in peptide bonding (chapter 4) -✓✓angle around the α-carbon -
carbonyl carbon bond
