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Bio-informatica stappenplan met vragen, af te drukken voor tijdens het openboek examen | NCBI, BLAST, FASTA | Hogeschool Gent | 2025/26

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Study summary for the Bio-informatica course at Hogeschool Gent covering practical bioinformatics tools and databases. The document walks through 13 key exam questions on FASTA format, OMIM database queries, NCBI genome searches, BLAST protein alignment, sequence analysis, and ORF identification—each with step-by-step solution methods. This is an effective exam preparation resource that organizes the course material by problem type, showing exactly how to navigate bioinformatics databases and tools to find correct answers.

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Samenvatting bio-informatica
1. Which of the following represents a sequence in FASTA format

- >
MPRAENWDEAEVGAEEAGVEEYGPEEDGGEEDGAEESGPEESGPEELGAEEEMEAGRPRPVLRSVNSPR
E….

1. Cursus opzoeken
2. via ctrl+f FASTA opzoeken in cursus
3. daar zien we dat FASTA moet beginnen met een groter dan >

LET OP! zie dat er geen puntje achter > staat


2. What does the symbol % mean in %100820 → OMIM

- None of the above




3. Who was the first to describe ACHOO or EHLIOOPHTHALMIC syndrome as written in the OMIM
database

- Everett

1. OMIM
2. ACHOO ingeven
3. ctrl + F
4. namen ingeven
5. kijken naar data bij de namen


4. Your lab supervisor tell you to go to the NCBI Human Genome page. What does she probably want
you to do?


- Identify a DNA sequence and see if it came from a human
-

5. Vermeulen S, when under supervision of Marc Mareel, published an article regarding the
importance of accurate cell culturing. Which cell cultures did he use in that article in fig 1?

- Only colon cancer cell lines
1. Artikel opzoeken op chamillo
2. Kijken naar figuur 1
3. In titel van figuur staat het

, 6. Friedel Nollet Fransiska Malfait and Franki Speleman co-authored a paper in 1999 in which specific
peptides were used to produce antibodies against an certain complex of proteins. The peptide used
was: PGDSNQLAWFDTDL. What is the most probable protein against which the antibody will react

- Ankyrin repeat and SOCS box protein 16 [Homo sapiens], Sequence ID: NP_543139

1.NCBI
2.BLAST -> protein blast
3.HVDPVQALSEFK intikken en vergelijken met de NP en XP
4. kijken wat het hoogste procent heeft (gelijkenissen bekijken)


7. Which of the following options represents the sig_peptide in NM_001903

- None of the others

1. NCBI
2. gene
3. voer NM_001903 in
4. klik op RefSeq
5. recentste vorm nemen (kortste NM)
6. Op NM_001903.5 tikken (in nieuw tablad openen)
7. kijken naar onderstreepte woorden aan linker kant


8. which statement is correct?

- BLAST, galaxy, Primer3, IGV are apps

Zie cursus
-> Via Ctrl+f "apps"


9. We investigated the following sequence in Dasypus novemcinctus:
CAATAGCTGACGTGTGCAGAAGTCCTTCTTGCCCTGGTCGTCGTTCCTGTCTGTCCAAGTACCAGTTCCC
ATTTGACCTGAGGGCCAGTGGGCCTGCGGCAGAGGGAAGAAGAAACAGGAAAAGGTTGCTGTACAACGCC
AGGAAAGAGAAGAGGAAGGAAATTAAGCTCTATTGGTGAAGGTCAATTCGAGGGTCTGCTGTAGCAGGTG
GCACCACTTGAAGGAGGGAGGAGGAAGTTTTCCGGATCCGTAGAGGTTTACCTCCAGTGTCAGCTATTGT
GGCCTTGAAGTGACTGAAGATCACCACTCTCCACAGGCCTGCTCCCAGGCCCACAGCCATCTTCCCCCAG
TTCGAGTGACTGCTCTGTTCCTTGGTCCCTGCTGTTGTCAGGGACGATTGCATGGGCTACGCCAGGAAAG
TAGGCTGGGTGACTGCAGGACTGGTGATTGGGGCTGGTGCTTGCTATTGCATTTATAAACTGACGCGTGG
AAGAAAACAGAACAAGGAAAAAATGGCTGAGGGTGGATCTGGGGATGTGGATGATGCTGGGGACTGTTCT
GGGGTCAGGTACAATGACTGGTCTGATGATGATGATGACGACAATGAGAGCAAGGATATAGTATGGTACC
CACCTTGGGCCCGGATTGGGACTGAGGCTGGGACCAGAGCCAGGGCCAGGGCCAGGGCCAGGGCCAACCG
GGCCCGTCGGGCTGTTCAGAAACGGGCCTCCCCCAATTCAGATGATACTATTTTGTCCCCTCAAGAACTG
CAGAAAGTTCTTTGCTTGGTTGAGATGTCTGAAAAGCCTTATATTCTTGAAGCAGCTTTAATTGCTCTAG

What is the cDNA accession number with a perfect match? XM_012525416

1. NCBI
2. Blast
3. nucleotide Blast
4. CAAT... invoegen
5. kijken bij "discriptions" naar beste match = (100%)

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