BIOL 134 - Final Practium study guide | Questions
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Terms in this set (45)
Conversions kilo-10^3(1000)
Milli-10^-3(.001)
Micro(0.000001)
Nano(0.000000001)
G to kg- 1g to 0.001 kg
m to cm: 1m to 100cm
m to mm: 1m to 1000mm
L to ml: 1L to 1000ml
Spectroscopy(why was the riboflavin Riboflavin solution is colored because it absorbs
solution yellow and the NaCl light in the blue region
solution clear?) the NaCl solution is clear because it is an ionic
compound and they do not absorb any visible light
Pseudomonas Aeruginosa (DNA bacteria(prokaryotic )
isolation)
Why are Proteinase K and Rnase Proteinase K - is an enzyme used to digest proteins
used in the isolation of DNA Rnase- protein that cuts RNA
How is genomic DNA removed from Centrifuge the plasmid DNA and keep the liquid
solution during the isolation of then add Na-acetate and ethanol and will give you
plasmid DNA? your purified DNA
, Pellet heavier and dense material collects at the bottom
Supernatant liquid remaining in the solution
What are the properties of restriction cut and cleaves DNA Molecules ,targets a specific
enzymes? sequences
do restriction enzymes target yes
specific enzyme
Why are restriction enzymes useful in you are able to get the specific part of the DNA
gene cloning? that you want to clone
What are sticky ends overhangs after cleaving the DNA in restriction
enzyme
What are the properties of plasmids circular DNA molecules , multiple cloning sites,
used for gene cloning? small, origin of replication , can have a resistance
gene
What are multiple cloning sites that contains serval restiction enzymes sites
located adjacent to each other to help facilate
cloning
Blue and white screening Blue colonies - contain plasmids with no inserts (if
the cells have beta-glactosidase)
white colonies- contain plasmids with the inserts
(no beta-galactosidase)
relies on LacZ- that encodes a small peptide
component of beta glactosidase
X-gal- specific sugar added to agar plate
and Answers | Verified Solutions | 2026 Edition |
Pass Guaranteed
Save
Terms in this set (45)
Conversions kilo-10^3(1000)
Milli-10^-3(.001)
Micro(0.000001)
Nano(0.000000001)
G to kg- 1g to 0.001 kg
m to cm: 1m to 100cm
m to mm: 1m to 1000mm
L to ml: 1L to 1000ml
Spectroscopy(why was the riboflavin Riboflavin solution is colored because it absorbs
solution yellow and the NaCl light in the blue region
solution clear?) the NaCl solution is clear because it is an ionic
compound and they do not absorb any visible light
Pseudomonas Aeruginosa (DNA bacteria(prokaryotic )
isolation)
Why are Proteinase K and Rnase Proteinase K - is an enzyme used to digest proteins
used in the isolation of DNA Rnase- protein that cuts RNA
How is genomic DNA removed from Centrifuge the plasmid DNA and keep the liquid
solution during the isolation of then add Na-acetate and ethanol and will give you
plasmid DNA? your purified DNA
, Pellet heavier and dense material collects at the bottom
Supernatant liquid remaining in the solution
What are the properties of restriction cut and cleaves DNA Molecules ,targets a specific
enzymes? sequences
do restriction enzymes target yes
specific enzyme
Why are restriction enzymes useful in you are able to get the specific part of the DNA
gene cloning? that you want to clone
What are sticky ends overhangs after cleaving the DNA in restriction
enzyme
What are the properties of plasmids circular DNA molecules , multiple cloning sites,
used for gene cloning? small, origin of replication , can have a resistance
gene
What are multiple cloning sites that contains serval restiction enzymes sites
located adjacent to each other to help facilate
cloning
Blue and white screening Blue colonies - contain plasmids with no inserts (if
the cells have beta-glactosidase)
white colonies- contain plasmids with the inserts
(no beta-galactosidase)
relies on LacZ- that encodes a small peptide
component of beta glactosidase
X-gal- specific sugar added to agar plate
