ACS BIOCHEMISTRY PRACTICE FINAL
FGCU BEHARRY COTICONE PACKAGE
DEAL EXAM SCRIPT 2026 FULL QUESTIONS
WITH TESTED ANSWERS GRADED A+
⩥ thioester bond (1).
Answer: compounds with functional group C-S-CoA-C (eg, in acetyl-
CoA)
⩥ zwitterion (3).
Answer: dipolar ion with both positive and negative groups but overall
neutral charge; can act as either an acid or base
⩥ Isoelectric point (pI) (chapter 3).
Answer: the characteristic pH at which the net electric charge is zero
⩥ SDS - Sodium Dodecyl Sulfate (chap 3).
Answer: detergent used to unfold proteins and give them uniform
negative charge
⩥ SDS Page (3).
, Answer: chromatography used to separate proteins based on mass. light
proteins travel fast than heavier ones
⩥ PCR - Polymerase chain reaction (3).
Answer: copies DNA multiple times to increase sample size
⩥ Isoelectric focusing (chapt 3).
Answer: procedure used to determine the isoelectric pt (pI) of a protein.
Protein migrates through gel until pH = pI (net charge = 0)
⩥ Two-Dimensional Electrophoresis (3).
Answer: combines isoelectric focusing and SDS electrophoresis;
separates proteins by both molecular weight and pI
⩥ specific activity (3).
Answer: number of enzyme units per mg of total protein (a measure of
enzyme purity)
⩥ activity (3).
Answer: total units of a certain enzyme in a solution
⩥ Peptide bonds (chapter 4).
FGCU BEHARRY COTICONE PACKAGE
DEAL EXAM SCRIPT 2026 FULL QUESTIONS
WITH TESTED ANSWERS GRADED A+
⩥ thioester bond (1).
Answer: compounds with functional group C-S-CoA-C (eg, in acetyl-
CoA)
⩥ zwitterion (3).
Answer: dipolar ion with both positive and negative groups but overall
neutral charge; can act as either an acid or base
⩥ Isoelectric point (pI) (chapter 3).
Answer: the characteristic pH at which the net electric charge is zero
⩥ SDS - Sodium Dodecyl Sulfate (chap 3).
Answer: detergent used to unfold proteins and give them uniform
negative charge
⩥ SDS Page (3).
, Answer: chromatography used to separate proteins based on mass. light
proteins travel fast than heavier ones
⩥ PCR - Polymerase chain reaction (3).
Answer: copies DNA multiple times to increase sample size
⩥ Isoelectric focusing (chapt 3).
Answer: procedure used to determine the isoelectric pt (pI) of a protein.
Protein migrates through gel until pH = pI (net charge = 0)
⩥ Two-Dimensional Electrophoresis (3).
Answer: combines isoelectric focusing and SDS electrophoresis;
separates proteins by both molecular weight and pI
⩥ specific activity (3).
Answer: number of enzyme units per mg of total protein (a measure of
enzyme purity)
⩥ activity (3).
Answer: total units of a certain enzyme in a solution
⩥ Peptide bonds (chapter 4).
