FRESENIUS BIOMED 2 PRACTICUM
EXAM QUESTIONS AND ANSWERS
Where are dialysate culture samples taken from? - Correct Answers -Either from the
blue Hansen connector, or from the dialyzer output port (Red)
When are dialysate culture samples to be taken? - Correct Answers -After the machine
has reached operating parameters for conductivity and temperature but before daily
disinfection.
How long must the RO System be in operation before obtaining samples? - Correct
Answers -At least 15 minutes
What are the FKC Action level and Allowable limit for Water Endotoxin Samples? -
Correct Answers -Action Level: 0.125 EU/ml
Allowable Limit: 0.25 EU.ml
What are the AAMI Action level and Allowable limit for Dialysate Colony Count
Samples? - Correct Answers -Action Level: 50 CFU/ml
Allowable Limit: 200 CFU/ml
What are the AAMI Action level and Allowable limit for Dialysate Endotoxin Samples? -
Correct Answers -Action Level: 1.0 EU/ml
Allowable Limit: 2 EU/ml
Can water samples be collected while patient treatments are being performed? -
Correct Answers -Yes, only where the sample collection process will not affect patient
treatment in any manner
How long should a sample port be rinsed before attempting to collect a sample from it? -
Correct Answers -2 minutes, to remove any residual contaminants
What are the conditions for dialysate sampling? - Correct Answers -No patient currently
dialyzing, machine is running in stable conductivity, and prior to daily cleaning and
disinfection
What is Scheduled Sampling for In-center dialysate sample collection? - Correct
Answers -A minimum of 2 machines per month and enough machines so all machines,
, including back up equipment, are tested at a minimum of once per year// or when
designated by a Dialysate Action Plan.
After installation of a new machine, how long will samples need to be taken? - Correct
Answers -Baseline sampling is done weekly for 4 consecutive weeks. Equipment may
be put into service if the week one sample result comes back under FKC action level,
but will continue to be sampled for the 4 consecutive weeks
When is a dialysis machine considered "idle" and will need to undergo validation
cultures? - Correct Answers -When a machine has not been disinfected for 7 days or
longer.
What is Scheduled Sampling for In-center water sample collection? - Correct Answers -
Water microbiological samples are to be collected no less than monthly, no more than
96 hours before system disinfection.
What are the FKC Action level and Allowable limit for Dialysate Endotoxin Samples? -
Correct Answers -Action Level: 0.06 EU/ml
Allowable limit: 0.25 EU/ml
What are the FKC Action level and Allowable limit for Dialysate Colony Count Samples?
- Correct Answers -Action Level: 20 CFU/ml
Allowable Limit: 100 CFU/ml
What are the FKC Action level and Allowable limit for Water Colony Count Samples? -
Correct Answers -Action Level: 20 CFU/ml
Allowable Limit: 100 CFU/ml
What are the AAMI Action level and Allowable limit for Water Colony Count Samples? -
Correct Answers -Action Level: 50 CFU/ml
Allowable Limit: 200 CFU/ml
What are the AAMI Action level and Allowable limit for Water Endotoxin Samples? -
Correct Answers -Action Level: 1 EU/ml
Allowable Limit: 2 EU/ml
What is the Micro Tool Box? - Correct Answers -A set of tools, guidelines, processes for
remediation in clinics with historical microbiological sampling result above action level.
How long should you wait to check for culture results back from Korus? - Correct
Answers -48 hours
What is the maximum time permitted to freeze samples for LAL testing? - Correct
Answers -Samples are no longer frozen whatsoever
EXAM QUESTIONS AND ANSWERS
Where are dialysate culture samples taken from? - Correct Answers -Either from the
blue Hansen connector, or from the dialyzer output port (Red)
When are dialysate culture samples to be taken? - Correct Answers -After the machine
has reached operating parameters for conductivity and temperature but before daily
disinfection.
How long must the RO System be in operation before obtaining samples? - Correct
Answers -At least 15 minutes
What are the FKC Action level and Allowable limit for Water Endotoxin Samples? -
Correct Answers -Action Level: 0.125 EU/ml
Allowable Limit: 0.25 EU.ml
What are the AAMI Action level and Allowable limit for Dialysate Colony Count
Samples? - Correct Answers -Action Level: 50 CFU/ml
Allowable Limit: 200 CFU/ml
What are the AAMI Action level and Allowable limit for Dialysate Endotoxin Samples? -
Correct Answers -Action Level: 1.0 EU/ml
Allowable Limit: 2 EU/ml
Can water samples be collected while patient treatments are being performed? -
Correct Answers -Yes, only where the sample collection process will not affect patient
treatment in any manner
How long should a sample port be rinsed before attempting to collect a sample from it? -
Correct Answers -2 minutes, to remove any residual contaminants
What are the conditions for dialysate sampling? - Correct Answers -No patient currently
dialyzing, machine is running in stable conductivity, and prior to daily cleaning and
disinfection
What is Scheduled Sampling for In-center dialysate sample collection? - Correct
Answers -A minimum of 2 machines per month and enough machines so all machines,
, including back up equipment, are tested at a minimum of once per year// or when
designated by a Dialysate Action Plan.
After installation of a new machine, how long will samples need to be taken? - Correct
Answers -Baseline sampling is done weekly for 4 consecutive weeks. Equipment may
be put into service if the week one sample result comes back under FKC action level,
but will continue to be sampled for the 4 consecutive weeks
When is a dialysis machine considered "idle" and will need to undergo validation
cultures? - Correct Answers -When a machine has not been disinfected for 7 days or
longer.
What is Scheduled Sampling for In-center water sample collection? - Correct Answers -
Water microbiological samples are to be collected no less than monthly, no more than
96 hours before system disinfection.
What are the FKC Action level and Allowable limit for Dialysate Endotoxin Samples? -
Correct Answers -Action Level: 0.06 EU/ml
Allowable limit: 0.25 EU/ml
What are the FKC Action level and Allowable limit for Dialysate Colony Count Samples?
- Correct Answers -Action Level: 20 CFU/ml
Allowable Limit: 100 CFU/ml
What are the FKC Action level and Allowable limit for Water Colony Count Samples? -
Correct Answers -Action Level: 20 CFU/ml
Allowable Limit: 100 CFU/ml
What are the AAMI Action level and Allowable limit for Water Colony Count Samples? -
Correct Answers -Action Level: 50 CFU/ml
Allowable Limit: 200 CFU/ml
What are the AAMI Action level and Allowable limit for Water Endotoxin Samples? -
Correct Answers -Action Level: 1 EU/ml
Allowable Limit: 2 EU/ml
What is the Micro Tool Box? - Correct Answers -A set of tools, guidelines, processes for
remediation in clinics with historical microbiological sampling result above action level.
How long should you wait to check for culture results back from Korus? - Correct
Answers -48 hours
What is the maximum time permitted to freeze samples for LAL testing? - Correct
Answers -Samples are no longer frozen whatsoever
