Chapter - Clinical Chemistry Study
Revision Questions and Answers 2026
Which of the following lamps provides a continuous spectrum of radiant energy in the visible,
near IR, and near UV regions of the spectrum?
A. Tungsten-filament
B. Hydrogen
C. Deuterium
D. Mercury Vapor - correct answer: A. Tungsten-filament
Which of the following isolates light within a narrow region of the spectrum?
A. Photomultiplier tube
B. Monochromator
C. Photovoltaic cell
D. Detector - correct answer: B. Monochromator
Which of the following is not descriptive of a photomultiplier tube?
A. Emits electrons proportionally to initial light absorbed
B. Must be shielded from stray light
C. Cannot be used with a chopper
D. Amplifies the initial signal received - correct answer: C. Cannot be used with a chopper
Which of the following is false about a photomultiplier tube?
,A. Converts radiant energy (light) to electrical energy (current)
B. Amplifies the current significantly
C. Has a very rapid response time
D. Is composed of an iron plate and a layer of selenium - correct answer: D. Is composed of an
iron plate and a layer of selenium
Which type of photodetector employs a linear arrangement that allows it to respond to a
specific wavelength resulting in complete UV/visible spectrum analysis?
A. Photomultiplier tube
B. Phototube
C. Barrier layer cell
D. Photodiode array - correct answer: D. Photodiode array
When performing spectrophotometer quality assurance checks, what is the holmium oxide glass
filter used to assess?
A. Linearity
B. Stray light
C. Absorbance accuracy
D. Wavelength accuracy - correct answer: D. Wavelength accuracy
In spectrophotometric analysis, what is the purpose of the reagent blank?
A. Correct for interfering chromogens
B. Correct for lipemia
,C. Correct for protein
D. Correct for color contribution of the reagents - correct answer: D. Correct for color
contribution of the reagents
In regard to bichromatic analysis, which of the following is false?
A. Absorbance is measured at the spectral absorbance peak for a blank and the sample using
the same wavelength.
B. Eliminates background interferences
C. Sample concentration determined from difference in two measured absorbances
D. Functions as a reference blank for each sample - correct answer: A. Absorbance is measured
at the spectral absorbance peak for a blank and the sample using the same wavelength.
The bandpass of a spectrophotometer is 10 nm. If an instrument is set at 540 nm, the
wavelengths that are permitted to impinge on the sample will be within what wavelength
range?
A. 530-540 nm
B. 530-550 nm
C. 535-545 nm
D. 540-550 nm - correct answer: C. 535-545 nm
Which of the following formulas is an expression of the Beer-Lambert law that is routinely
applied to spectrophotometric analysis?
A. Aᵤ X (Cs/As) = Cᵤ
B. Cᵤ X (Cs/As) = Aᵤ
C. As X (Cs/Cᵤ) = Aᵤ
, D. A = 2 - log %T - correct answer: A. Aᵤ X (Cs/As) = Cᵤ
In spectrophotometry, which of the
following is a mathematical expression of the relationship between absorbance and
transmittance?
A. A = abc
B. (Aᵤ/Cᵤ) = (As/Cs)
C. A = 2 - log %T
D. A = log %T - correct answer: C. A = 2 - log %T
Which of the following is not a problem inherent in turbidimetry?
A. Variation in particle size of samples
B. Variation in particle size of standards
C. Rate of aggregation or settling of particles
D. Need to maintain a constant and specific temperature - correct answer: D. Need to maintain
a constant and specific temperature
Which of the following may be associated with reflectance spectrophotometry as it relates to
the dry reagent slide technique?
A. Light projected to the slide at 180-degree angle
B. Dye concentration directly proportional to reflectance
C. Unabsorbed, reflected light detected by photodetector
D. Reflectance values are linearly proportional to transmission values - correct answer: C.
Unabsorbed, reflected light detected by photodetector
Revision Questions and Answers 2026
Which of the following lamps provides a continuous spectrum of radiant energy in the visible,
near IR, and near UV regions of the spectrum?
A. Tungsten-filament
B. Hydrogen
C. Deuterium
D. Mercury Vapor - correct answer: A. Tungsten-filament
Which of the following isolates light within a narrow region of the spectrum?
A. Photomultiplier tube
B. Monochromator
C. Photovoltaic cell
D. Detector - correct answer: B. Monochromator
Which of the following is not descriptive of a photomultiplier tube?
A. Emits electrons proportionally to initial light absorbed
B. Must be shielded from stray light
C. Cannot be used with a chopper
D. Amplifies the initial signal received - correct answer: C. Cannot be used with a chopper
Which of the following is false about a photomultiplier tube?
,A. Converts radiant energy (light) to electrical energy (current)
B. Amplifies the current significantly
C. Has a very rapid response time
D. Is composed of an iron plate and a layer of selenium - correct answer: D. Is composed of an
iron plate and a layer of selenium
Which type of photodetector employs a linear arrangement that allows it to respond to a
specific wavelength resulting in complete UV/visible spectrum analysis?
A. Photomultiplier tube
B. Phototube
C. Barrier layer cell
D. Photodiode array - correct answer: D. Photodiode array
When performing spectrophotometer quality assurance checks, what is the holmium oxide glass
filter used to assess?
A. Linearity
B. Stray light
C. Absorbance accuracy
D. Wavelength accuracy - correct answer: D. Wavelength accuracy
In spectrophotometric analysis, what is the purpose of the reagent blank?
A. Correct for interfering chromogens
B. Correct for lipemia
,C. Correct for protein
D. Correct for color contribution of the reagents - correct answer: D. Correct for color
contribution of the reagents
In regard to bichromatic analysis, which of the following is false?
A. Absorbance is measured at the spectral absorbance peak for a blank and the sample using
the same wavelength.
B. Eliminates background interferences
C. Sample concentration determined from difference in two measured absorbances
D. Functions as a reference blank for each sample - correct answer: A. Absorbance is measured
at the spectral absorbance peak for a blank and the sample using the same wavelength.
The bandpass of a spectrophotometer is 10 nm. If an instrument is set at 540 nm, the
wavelengths that are permitted to impinge on the sample will be within what wavelength
range?
A. 530-540 nm
B. 530-550 nm
C. 535-545 nm
D. 540-550 nm - correct answer: C. 535-545 nm
Which of the following formulas is an expression of the Beer-Lambert law that is routinely
applied to spectrophotometric analysis?
A. Aᵤ X (Cs/As) = Cᵤ
B. Cᵤ X (Cs/As) = Aᵤ
C. As X (Cs/Cᵤ) = Aᵤ
, D. A = 2 - log %T - correct answer: A. Aᵤ X (Cs/As) = Cᵤ
In spectrophotometry, which of the
following is a mathematical expression of the relationship between absorbance and
transmittance?
A. A = abc
B. (Aᵤ/Cᵤ) = (As/Cs)
C. A = 2 - log %T
D. A = log %T - correct answer: C. A = 2 - log %T
Which of the following is not a problem inherent in turbidimetry?
A. Variation in particle size of samples
B. Variation in particle size of standards
C. Rate of aggregation or settling of particles
D. Need to maintain a constant and specific temperature - correct answer: D. Need to maintain
a constant and specific temperature
Which of the following may be associated with reflectance spectrophotometry as it relates to
the dry reagent slide technique?
A. Light projected to the slide at 180-degree angle
B. Dye concentration directly proportional to reflectance
C. Unabsorbed, reflected light detected by photodetector
D. Reflectance values are linearly proportional to transmission values - correct answer: C.
Unabsorbed, reflected light detected by photodetector
