Portage Learning MICRO 100 microbiology EXAM A+ GRADE ASSURED COMPLETE SOLUTIONS AND VERIFIED ANSWERS

PORTAGE LEARNING EXAM vc vc




Exam Solution
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Microbiology Portage Module 4 Microbial Growth and C vc vc vc vc vc vc vc




ontrol 2026 A+ GRADE ASSURED COMPLETE SOLUTION
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S AND VERIFIED ANSWERS (A24B6)
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QUESTION 1 vc




Expanding a given microbial population allows researchers to do what?
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ANSWER

to detect and assess the microorganism more easily
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QUESTION 2 vc




What is Growth Media?
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ANSWER

Growth media is a liquid or solid formulated to support microbial growth.
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QUESTION 3 vc




What does Growth Media contain?
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ANSWER

The media contains essential nutrients to provide the microbe with a source of carbon/energy. The nutr
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ient composition often includes a rich source of sugars, amino acids and vitamins.
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QUESTION 4 vc




What else can Growth Media also be called?
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ANSWER

Such media can also be referred to as a nutrient broth and is commonly used to grow microbes in a sus
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pension.

,QUESTION 5 vc




Describe LB media vc vc




ANSWER

Perhaps the most common nutrient broth in the lab, LB media (lysogeny broth) is a nutritionally rich liq
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uid known for its ability to grow a vast array of microbes.
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QUESTION 6 vc




What is Selective Media? vc vc vc




ANSWER

Selective media allows for only the growth of certain microbes and, by extension, restricts the growth of
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all others.
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QUESTION 7 vc




How can Selective Media be accomplished?
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ANSWER

Such an approach can be accomplished in a variety of ways including: using limiting amounts of nutrien
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ts, varying degrees of pH (being either very acidic or very basic media), or various chemical additives th
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at limit unwanted microbial growth (i.e.) antibiotics.
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QUESTION 8 vc




Describe how Selective Media works with Gram-Positive versus Gram-Negative bacteria.
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ANSWER

If a plate is selective for Gram-Negative then it will only grow Gram-
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Negative bacteria on it. It will not grow Gram-Positive bacteria on it.
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QUESTION 9 vc




What is Differential Media?
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ANSWER

Differential media distinguishes between two, often related, microbes.
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QUESTION 10 vc

, What is one way that a Differential Media can distinguish between Escherichia coli and S
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almonella?
ANSWER

They are both Gram negative but can be distinguished by the presence (or absence) of lactose fermentat
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ion. If grown on the same differential media, E coli ferments lactose and turns the culture red. In contra
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st, Salmonella does not ferment lactose and the culture remains white/tan in color. (This above example
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is probably on MacConkey agar)
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QUESTION 11 vc




What is a fastidious microorganism?
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ANSWER

an organism with complex growth requirements such that if absent it will not grow
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QUESTION 12 vc




What are Enriched Medias used for?
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ANSWER

Enriched media is used to grow fastidious microorganisms
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QUESTION 13 vc




Enriched Medias contain what? vc vc vc




ANSWER

enriched medias contain the essential nutrients required for the growth of this subset of microorganism
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s



QUESTION 14 vc




Can a plate be a combination of selective, differential and enriched?
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ANSWER

Yes



QUESTION 15 vc




Growth Media comes in what 2 forms?
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ANSWER