PORTAGE LEARNING EXAM vc vc
Exam Solution vc
Portage Learning microbiology module 3 2026 A+ GRA vc vc vc vc vc vc vc
DE ASSURED COMPLETE SOLUTIONS AND VERIFIED ANS
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WERS (3B327) vc
QUESTION 1 vc
A nanometer is defined as:
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ANSWER
10-9 A nanometer is defined as one-billionth of a meter
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QUESTION 2 vc
True or False: A nanometeris longer than a micrometer
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ANSWER
False. A nanometer is 1,000 times smaller than a micrometer.
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QUESTION 3 vc
Resolution and contrast are two critical factors that influence your ability to see an objec
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t. Explain each.
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ANSWER
Resolution refers to the distance between two objects at which the objects still can be seen as separate.
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Poor or low resolution means two (or more) objects may appear as one. *Contrast is the difference in li
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ght absorbance between two objects. Poor contrast gives a high background and makes the visualization
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of multiple objects difficult. For instance, trying to identify 2 dark colored objects at night (low light = l
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ow contrast) versus the same 2 objects in the middle of a sunny afternoon (bright light against 2 dark o
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bjects = high contrast).
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QUESTION 4 vc
, Assuming a constant (non- vc vc vc
adjustable) light source power, identify the part of the microscope you would adjust to li
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mit the amount of light entering the microscope.
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ANSWER
The iris controls the amount of light that passes through the sample and into the objective lens. Thus, it
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can be adjusted (opened or closed) to alter the amount of light.
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QUESTION 5 vc
What is the total magnification (relative to your eye) of a sample imaged with a 60x obje
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ctive and a 10x eyepiece? Show your math
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ANSWER
60 x 10 = 600x magnification
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QUESTION 6 vc
True or False: Staining is often required to image a cell that is adherent and flat (thin).
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ANSWER
True. Adherent, flat and unstained cells are almost invisible due to the limits on both resolution and con
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trast. Therefore, cell staining is often required to adequately image the samp
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QUESTION 7 vc
Which of the following could NOT be seen clearly by the unaided eye? Select all that appl
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y.
ANSWER
24um and 0.2um. The unaided eye can, on average, clearly resolve objects > 100 μm
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QUESTION 8 vc
This type of microscope utilizes ultraviolet (UV) light to illuminate stained objects.
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ANSWER
Fluorescence
QUESTION 9 vc
This type of microscope uses a specialized condenser and objective to amplify the slight
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differences between cells and background vc vc vc vc
Exam Solution vc
Portage Learning microbiology module 3 2026 A+ GRA vc vc vc vc vc vc vc
DE ASSURED COMPLETE SOLUTIONS AND VERIFIED ANS
vc vc vc vc vc vc
WERS (3B327) vc
QUESTION 1 vc
A nanometer is defined as:
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ANSWER
10-9 A nanometer is defined as one-billionth of a meter
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QUESTION 2 vc
True or False: A nanometeris longer than a micrometer
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ANSWER
False. A nanometer is 1,000 times smaller than a micrometer.
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QUESTION 3 vc
Resolution and contrast are two critical factors that influence your ability to see an objec
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t. Explain each.
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ANSWER
Resolution refers to the distance between two objects at which the objects still can be seen as separate.
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Poor or low resolution means two (or more) objects may appear as one. *Contrast is the difference in li
vc vc vc vc vc vc vc vc vc vc vc vc vc vc vc vc vc vc
ght absorbance between two objects. Poor contrast gives a high background and makes the visualization
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of multiple objects difficult. For instance, trying to identify 2 dark colored objects at night (low light = l
vc vc vc vc vc vc vc vc vc vc vc vc vc vc vc vc vc vc vc
ow contrast) versus the same 2 objects in the middle of a sunny afternoon (bright light against 2 dark o
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bjects = high contrast).
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QUESTION 4 vc
, Assuming a constant (non- vc vc vc
adjustable) light source power, identify the part of the microscope you would adjust to li
vc vc vc vc vc vc vc vc vc vc vc vc vc vc
mit the amount of light entering the microscope.
vc vc vc vc vc vc vc
ANSWER
The iris controls the amount of light that passes through the sample and into the objective lens. Thus, it
vc vc vc vc vc vc vc vc vc vc vc vc vc vc vc vc vc vc vc
can be adjusted (opened or closed) to alter the amount of light.
vc vc vc vc vc vc vc vc vc vc vc
QUESTION 5 vc
What is the total magnification (relative to your eye) of a sample imaged with a 60x obje
vc vc vc vc vc vc vc vc vc vc vc vc vc vc vc vc
ctive and a 10x eyepiece? Show your math
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ANSWER
60 x 10 = 600x magnification
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QUESTION 6 vc
True or False: Staining is often required to image a cell that is adherent and flat (thin).
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ANSWER
True. Adherent, flat and unstained cells are almost invisible due to the limits on both resolution and con
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trast. Therefore, cell staining is often required to adequately image the samp
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QUESTION 7 vc
Which of the following could NOT be seen clearly by the unaided eye? Select all that appl
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y.
ANSWER
24um and 0.2um. The unaided eye can, on average, clearly resolve objects > 100 μm
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QUESTION 8 vc
This type of microscope utilizes ultraviolet (UV) light to illuminate stained objects.
vc vc vc vc vc vc vc vc vc vc vc
ANSWER
Fluorescence
QUESTION 9 vc
This type of microscope uses a specialized condenser and objective to amplify the slight
vc vc vc vc vc vc vc vc vc vc vc vc vc vc
differences between cells and background vc vc vc vc
