MIC 401 LAB PRACTICAL QUESTIONS AND VERIFIED
ANSWERS
Which of the following is an example of an antigen that would likely be recognized by a
normal-functioning human immune system?
- Answers - Covid19 Spike Protein & Bacterial Flagella
Which type of test typically requires significantly more antibody than antigen?
- Answers - Precipitation test
When performing an indirect ELISA, the ___ antibody will bind directly to the antigen.
- Answers - primary
A bacterial cell that looks like a bent rod, or comma shaped, would best be categorized
as?
- Answers - Vibrio
If you view a sample using the oil immersion lens, what is the total magnification of your
sample?
- Answers - 1000x
___ is an extract of seaweed used as a solidifying agent when making media such as
slants and plates.
- Answers - agar
If you performed a Gram stain on a gram negative cell but your bacteria appeared
colorless by the end, which reagent did you most likely forget to add?
- Answers - Safranin
If an organism in incapable of producing hemolysin, you would expect it to appear as
WHAT on blood agar plates?
- Answers - Gamma hemolytic
A break in the agar of a TSI slant indicates what?
- Answers - Gas production
, All of the following can be used to differentiate Staphylococcus species from
Streptococci EXCEPT?
a. Catalase Test
b. Gram stain reaction (color)
c. Growth pattern viewed under microscope
Which 2 tests are able to differentiate organisms based on the ability to ferment
lactose?
- Answers - MacConkey agar and TSI slants
An organism that can grow in the presence of oxygen but does not utilize it would best
be described as?
- Answers - Aerotolerant anaerobe
Which component of mannitol salt agar selects for Staph species?
- Answers - high salt content
A Kirby Bauer test is performed by measuring the zone of ___ around antibiotic disks
and determining susceptibility.
- Answers - inhibition
Immunogenicity (most to least)
- Proteins (complete antigen)
_ Polysaccharides (complete antigen)
- Lipids (complete antigen)
- Small molecules (Hapten - incomplete antigen)
Antigen
- molecule that stimulates an immune response
- 2 heavy 2 light chains
- Prod by B cells
Serology
ANSWERS
Which of the following is an example of an antigen that would likely be recognized by a
normal-functioning human immune system?
- Answers - Covid19 Spike Protein & Bacterial Flagella
Which type of test typically requires significantly more antibody than antigen?
- Answers - Precipitation test
When performing an indirect ELISA, the ___ antibody will bind directly to the antigen.
- Answers - primary
A bacterial cell that looks like a bent rod, or comma shaped, would best be categorized
as?
- Answers - Vibrio
If you view a sample using the oil immersion lens, what is the total magnification of your
sample?
- Answers - 1000x
___ is an extract of seaweed used as a solidifying agent when making media such as
slants and plates.
- Answers - agar
If you performed a Gram stain on a gram negative cell but your bacteria appeared
colorless by the end, which reagent did you most likely forget to add?
- Answers - Safranin
If an organism in incapable of producing hemolysin, you would expect it to appear as
WHAT on blood agar plates?
- Answers - Gamma hemolytic
A break in the agar of a TSI slant indicates what?
- Answers - Gas production
, All of the following can be used to differentiate Staphylococcus species from
Streptococci EXCEPT?
a. Catalase Test
b. Gram stain reaction (color)
c. Growth pattern viewed under microscope
Which 2 tests are able to differentiate organisms based on the ability to ferment
lactose?
- Answers - MacConkey agar and TSI slants
An organism that can grow in the presence of oxygen but does not utilize it would best
be described as?
- Answers - Aerotolerant anaerobe
Which component of mannitol salt agar selects for Staph species?
- Answers - high salt content
A Kirby Bauer test is performed by measuring the zone of ___ around antibiotic disks
and determining susceptibility.
- Answers - inhibition
Immunogenicity (most to least)
- Proteins (complete antigen)
_ Polysaccharides (complete antigen)
- Lipids (complete antigen)
- Small molecules (Hapten - incomplete antigen)
Antigen
- molecule that stimulates an immune response
- 2 heavy 2 light chains
- Prod by B cells
Serology
