ASCP CG COMPREHENSIVE REVIEW 2026
VERIFIED QUESTIONS AND SOLUTIONS
◉The following are indicators for amniocentesis except: Answer: A
family history of an autosomal recessive disorder.
◉When a bone marrow sample cannot be delivered to the lab within
4 hrs, the sample should: Answer: Be put in media and kept at room
temp.
◉A PB sample is clotted. What do you do? Answer: Break up the
clot.
◉All of the following information is required during the specimen
set up procedure except: Answer: Infectious agent.
◉When should samples be logged in? Answer: As soon as they
arrive in the laboratory.
◉How long do you wait before requesting an amniotic fluid if no cell
attachment is observed? Answer: 10 days.
,◉All of the following will result in culture failure except: Answer:
Transport at room temp.
◉Which cells would you want to use for long term serial cytogenetic
studies? Answer: Fibroblasts.
◉The following enzymes can be used to dissociate chorionic villus
samples except for: Answer: RNAse.
◉Which of the following is not a necessary ingredient which is
added to modified medium? Answer: Antibiotics.
◉All of the following have buffering capability except: Answer: L-
Glutamine.
◉What type of media is necessary for the culture of Fragile X cases?
Answer: Folic acid deficient media.
◉The most important reason why Biological Safety Caninets are
used instead of Horizontal Laminar Flow Hoods in the handling of
human tissues is: Answer: To protect the technologist who is
processing the specimen.
,◉In which of the following situations would one suspect bacterial
contamination? Answer: The culture media is turbid in appearance.
◉Which of the following can be used for serial culture from amniotic
fluid? Answer: Fibroblasts.
◉Which of the following chemicals is used for cryopreservation?
Answer: Dimethyl sulfoxide (DMSO).
◉Adding the drug Colcemid to cell cultures can cause all of the
following phenomenons to occur except: Answer: Cell swelling.
◉During slidemaking all of the following factors are important
except for: Answer: Slides are 1st being dipped in acetic acid.
◉A good banding pattern will be expected when we observe
chromosomes before banding under the phase scope with the
following appearance: Answer: Dark.
◉Which of the following factors are not important for chromosome
spreading? Answer: Mitotic index.
, ◉The best method to process slides for G-banding on the same day
that they are dropped is: Answer: Incubate the slide in 90°C for 1-3
hrs before banding.
◉You are analyzing a clinical case and discover a small extra marker
with satellites. What staining technique would be most useful in
determining the chromosomal origin of this marker? Answer:
DAPI/DA.
◉When using a fluorescein labeled probe during the FISH
procedure, the appropriate counter stain used should be: Answer:
Propidium iodide.
◉When doing sequential staining which of the following is used to
remove oil from slides? Answer: Xylene.
◉What is the best banding technique to distinguish between
47,XY,+18 and 47,XYY? Answer: QFQ.
◉When the identification of paired chromosomes is the primary
goal, which if the following banding techniques should be used?
Answer: G-banding.
VERIFIED QUESTIONS AND SOLUTIONS
◉The following are indicators for amniocentesis except: Answer: A
family history of an autosomal recessive disorder.
◉When a bone marrow sample cannot be delivered to the lab within
4 hrs, the sample should: Answer: Be put in media and kept at room
temp.
◉A PB sample is clotted. What do you do? Answer: Break up the
clot.
◉All of the following information is required during the specimen
set up procedure except: Answer: Infectious agent.
◉When should samples be logged in? Answer: As soon as they
arrive in the laboratory.
◉How long do you wait before requesting an amniotic fluid if no cell
attachment is observed? Answer: 10 days.
,◉All of the following will result in culture failure except: Answer:
Transport at room temp.
◉Which cells would you want to use for long term serial cytogenetic
studies? Answer: Fibroblasts.
◉The following enzymes can be used to dissociate chorionic villus
samples except for: Answer: RNAse.
◉Which of the following is not a necessary ingredient which is
added to modified medium? Answer: Antibiotics.
◉All of the following have buffering capability except: Answer: L-
Glutamine.
◉What type of media is necessary for the culture of Fragile X cases?
Answer: Folic acid deficient media.
◉The most important reason why Biological Safety Caninets are
used instead of Horizontal Laminar Flow Hoods in the handling of
human tissues is: Answer: To protect the technologist who is
processing the specimen.
,◉In which of the following situations would one suspect bacterial
contamination? Answer: The culture media is turbid in appearance.
◉Which of the following can be used for serial culture from amniotic
fluid? Answer: Fibroblasts.
◉Which of the following chemicals is used for cryopreservation?
Answer: Dimethyl sulfoxide (DMSO).
◉Adding the drug Colcemid to cell cultures can cause all of the
following phenomenons to occur except: Answer: Cell swelling.
◉During slidemaking all of the following factors are important
except for: Answer: Slides are 1st being dipped in acetic acid.
◉A good banding pattern will be expected when we observe
chromosomes before banding under the phase scope with the
following appearance: Answer: Dark.
◉Which of the following factors are not important for chromosome
spreading? Answer: Mitotic index.
, ◉The best method to process slides for G-banding on the same day
that they are dropped is: Answer: Incubate the slide in 90°C for 1-3
hrs before banding.
◉You are analyzing a clinical case and discover a small extra marker
with satellites. What staining technique would be most useful in
determining the chromosomal origin of this marker? Answer:
DAPI/DA.
◉When using a fluorescein labeled probe during the FISH
procedure, the appropriate counter stain used should be: Answer:
Propidium iodide.
◉When doing sequential staining which of the following is used to
remove oil from slides? Answer: Xylene.
◉What is the best banding technique to distinguish between
47,XY,+18 and 47,XYY? Answer: QFQ.
◉When the identification of paired chromosomes is the primary
goal, which if the following banding techniques should be used?
Answer: G-banding.
