Page 1 of 32
ASCP BOC EXAM/ MLS ASCP
CERTIFICATION
ACTUAL EXAM QUESTIONS AND
CORRECT ANSWERS
A consistently and repeatedly negative IFA-ANA is: - ✔✔✔ Correct
Answer > strong evidence against5 untreated SLE
What kind of antigen-antibody reaction would be expected if soluble
antigen is added to homologous antibody? - ✔✔✔ Correct Answer >
precipitation
the Philadelphia chromosome is formed by a translocation between the: -
✔✔✔ Correct Answer > long arm of chromosome 22 and long arm of
chromosome 9
Ten units of group A platelets were transfused to a group AB patient.
The pretransfusion platelet count was 12x10^3/uL and the
,Page 2 of 32
posttransfusion count was 18x10^3/uL. From this information, the
technologist would most likely conclude that the patient:
a) needs group AB platelets to be effective
b) clinical data does not suggest a need for platelets
c) has developed antibodies to the transfused platelets
d) should receive irradiated platelets - ✔✔✔ Correct Answer > has developed
antibodies to the transfused platelets
A solution contains 20 g of solute dissolved in 0.5 L of water.
What is the percentage of this solution? - ✔✔✔ Correct Answer > 4%
A technician is asked to clean out the chemical reagent storeroom and
discard any reagents not used in the past 5 years.
How should the technician proceed? - ✔✔✔ Correct Answer > consult
MSDS sheets for proper disposal
in a the clauss fibrinogen method, the time to clot formation in plasma is
measured after the addition of: - ✔✔✔ Correct Answer > thrombin
,Page 3 of 32
a centrifuge head has a diameter of 60 cm and spins at 3,000 RPM. What
is the maximum achievable G force? (G=0,00001 x radius in cm x RPM)
- ✔✔✔ Correct Answer > 2,700 G
A technologist spilled 10 gallons of formaldehyde on the floor. After
determining the chemical poses a significant health hazard, the first
action step would be to: - ✔✔✔ Correct Answer > Evacuate the area
A first morning sputum is received for culture of mycobacteria. It is
digested and concentrated by the N-acetyl-L-cysteine alkali method.
Two Lowenstein-Jensen slants are incubated in the dark at 35C with 5-
10% CO2. The smears revealed acid-fast bacilli, and after 7 days no
growth appears on the slants. The best explanation is: - ✔✔✔ Correct Answer
> Cultures held for insufficient length of time. (mycobacteria are slow
growers and may take up to 12 weeks)
In the assay of lactate dehydrogenase, which of the following products is
actually measured? - ✔✔✔ Correct Answer > NADH
, Page 4 of 32
A sputum specimen from a patient with a known Klebsiella pneumonia
infection is received in the lab for fungus culture. The proper procedure
for handling this specimen is to:
a. reject the current specimen and request a repeat culture when the
bacterial organism is no longer present
b. incubate culture tubes at RT in order to inhibit bacteria
c. include media that have cycloheximide and chloramphenicol to
inhibit bacteria and saprophytic fungi
d. perform direct PAS stain; if no fungal organism seen, reject specimen
- ✔✔✔ Correct Answer > c. include media that have cycloheximide and
chloramphenicol to inhibit bacteria and saprophytic fungi
Humoral antibodies are produced by which cells? - ✔✔✔ Correct
Answer > B Lymphocytes
What battery of tests is most useful in evaluating an anion gap of
22 mEq/L (22 mmol/L)? - ✔✔✔ Correct Answer > BUN, creatinine, salicylate
and methanol
ASCP BOC EXAM/ MLS ASCP
CERTIFICATION
ACTUAL EXAM QUESTIONS AND
CORRECT ANSWERS
A consistently and repeatedly negative IFA-ANA is: - ✔✔✔ Correct
Answer > strong evidence against5 untreated SLE
What kind of antigen-antibody reaction would be expected if soluble
antigen is added to homologous antibody? - ✔✔✔ Correct Answer >
precipitation
the Philadelphia chromosome is formed by a translocation between the: -
✔✔✔ Correct Answer > long arm of chromosome 22 and long arm of
chromosome 9
Ten units of group A platelets were transfused to a group AB patient.
The pretransfusion platelet count was 12x10^3/uL and the
,Page 2 of 32
posttransfusion count was 18x10^3/uL. From this information, the
technologist would most likely conclude that the patient:
a) needs group AB platelets to be effective
b) clinical data does not suggest a need for platelets
c) has developed antibodies to the transfused platelets
d) should receive irradiated platelets - ✔✔✔ Correct Answer > has developed
antibodies to the transfused platelets
A solution contains 20 g of solute dissolved in 0.5 L of water.
What is the percentage of this solution? - ✔✔✔ Correct Answer > 4%
A technician is asked to clean out the chemical reagent storeroom and
discard any reagents not used in the past 5 years.
How should the technician proceed? - ✔✔✔ Correct Answer > consult
MSDS sheets for proper disposal
in a the clauss fibrinogen method, the time to clot formation in plasma is
measured after the addition of: - ✔✔✔ Correct Answer > thrombin
,Page 3 of 32
a centrifuge head has a diameter of 60 cm and spins at 3,000 RPM. What
is the maximum achievable G force? (G=0,00001 x radius in cm x RPM)
- ✔✔✔ Correct Answer > 2,700 G
A technologist spilled 10 gallons of formaldehyde on the floor. After
determining the chemical poses a significant health hazard, the first
action step would be to: - ✔✔✔ Correct Answer > Evacuate the area
A first morning sputum is received for culture of mycobacteria. It is
digested and concentrated by the N-acetyl-L-cysteine alkali method.
Two Lowenstein-Jensen slants are incubated in the dark at 35C with 5-
10% CO2. The smears revealed acid-fast bacilli, and after 7 days no
growth appears on the slants. The best explanation is: - ✔✔✔ Correct Answer
> Cultures held for insufficient length of time. (mycobacteria are slow
growers and may take up to 12 weeks)
In the assay of lactate dehydrogenase, which of the following products is
actually measured? - ✔✔✔ Correct Answer > NADH
, Page 4 of 32
A sputum specimen from a patient with a known Klebsiella pneumonia
infection is received in the lab for fungus culture. The proper procedure
for handling this specimen is to:
a. reject the current specimen and request a repeat culture when the
bacterial organism is no longer present
b. incubate culture tubes at RT in order to inhibit bacteria
c. include media that have cycloheximide and chloramphenicol to
inhibit bacteria and saprophytic fungi
d. perform direct PAS stain; if no fungal organism seen, reject specimen
- ✔✔✔ Correct Answer > c. include media that have cycloheximide and
chloramphenicol to inhibit bacteria and saprophytic fungi
Humoral antibodies are produced by which cells? - ✔✔✔ Correct
Answer > B Lymphocytes
What battery of tests is most useful in evaluating an anion gap of
22 mEq/L (22 mmol/L)? - ✔✔✔ Correct Answer > BUN, creatinine, salicylate
and methanol
