UCF Biomed Exit Exam Test Questions and Answers Graded A
Showed that bacteria can acquire DNA through transfor-
mation
Griflth's experiment
Two strains of Pneumococcus; DNA from pathogenic strain
taken up by non-pathogenic strain, which then became
pathogenic
DNA, not proteins, caused bacterial transformation and is
Avery's experiment
therefore the carrier of genetic information
Meselson's experiment Showed that DNA replication is semi-conservative
McClintock's experiment Discovered transposons
Showed that only the DNA, no proteins, from bacterio-
phage enters a bacteria.
Hershey-Chase experiment
This demonstrates that genetic information is carried in
DNA.
Watson and Crick Generated the double-stranded helix model of DNA
Part of the DNA Pol. 3 holoenzyme
Sliding clamp
Prevents the polymerase from dissociating from the tem-
plate DNA strand
DNA Helicase Unwinds DNA, separating the two strands
Topoisomerase Alters the supercoiled form of DNA
Adds a species-dependent telomere repeat sequence to
Telomerase the 3' end of telomeres without the need for a DNA tem-
plate
Order in which enzymes participate in DNA replication
1/9
, UCF Biomed Exit Exam Test Questions and Answers Graded A
Topoisomerase
Helicase
Single-stranded binding proteins
Primase
DNA polymerase 3 holoenzyme
DNA polymerase 1
- Removes RNA primers and fills in gaps between Okazaki
fragments
Ligase
A DNA-binding proteins that recruit transcriptional ma-
Activator proteins
chinery
Enhancers The region of DNA that activators bind to
DNA sequences that repressor proteins bind to in order to
Silencers
decrease transcription
Regulatory RNA elements within the mRNA that act as
direct sensors of, small molecules, tRNA, temperature, pH,
Riboswitches
metals, altering the rate of production of the product of
that mRNA in prokaryotes
Transcription
Capping
- Added almost immediately after transcription begins
Processing of eukaryotic mRNA
Polyadenylation
RNA splicing
2/9
Showed that bacteria can acquire DNA through transfor-
mation
Griflth's experiment
Two strains of Pneumococcus; DNA from pathogenic strain
taken up by non-pathogenic strain, which then became
pathogenic
DNA, not proteins, caused bacterial transformation and is
Avery's experiment
therefore the carrier of genetic information
Meselson's experiment Showed that DNA replication is semi-conservative
McClintock's experiment Discovered transposons
Showed that only the DNA, no proteins, from bacterio-
phage enters a bacteria.
Hershey-Chase experiment
This demonstrates that genetic information is carried in
DNA.
Watson and Crick Generated the double-stranded helix model of DNA
Part of the DNA Pol. 3 holoenzyme
Sliding clamp
Prevents the polymerase from dissociating from the tem-
plate DNA strand
DNA Helicase Unwinds DNA, separating the two strands
Topoisomerase Alters the supercoiled form of DNA
Adds a species-dependent telomere repeat sequence to
Telomerase the 3' end of telomeres without the need for a DNA tem-
plate
Order in which enzymes participate in DNA replication
1/9
, UCF Biomed Exit Exam Test Questions and Answers Graded A
Topoisomerase
Helicase
Single-stranded binding proteins
Primase
DNA polymerase 3 holoenzyme
DNA polymerase 1
- Removes RNA primers and fills in gaps between Okazaki
fragments
Ligase
A DNA-binding proteins that recruit transcriptional ma-
Activator proteins
chinery
Enhancers The region of DNA that activators bind to
DNA sequences that repressor proteins bind to in order to
Silencers
decrease transcription
Regulatory RNA elements within the mRNA that act as
direct sensors of, small molecules, tRNA, temperature, pH,
Riboswitches
metals, altering the rate of production of the product of
that mRNA in prokaryotes
Transcription
Capping
- Added almost immediately after transcription begins
Processing of eukaryotic mRNA
Polyadenylation
RNA splicing
2/9
