______ is credited with the first practical forensic application of human DNA analysis. -
ANSWER-Alec Jeffreys
RFLP is ____ sensitive as PCR methods. - ANSWER-less
Which method uses the smallest fragments of DNA? - ANSWER-STR
CODIS looks for relationships between the profiles of unidentified remains and relatives
of missing persons. - ANSWER-True
short tandem repeats (STRs) are also known as _____ - ANSWER-Microsatellites
Which is not in DAB standard 6? - ANSWER-D. Amplified DNA does not need to be in
separate area than DNA extraction and PCR set-up.
When pipetting the pipet tip should be placed - ANSWER-at the top of the liquid sample
If you are LEFT handed, the order of movement would be as follows: - ANSWER-Left to
right
Which is NOT appropriate during sample preparation? - ANSWER-Process both
question samples and reference samples together.
What is NOT considered a type of eye protection? - ANSWER-Contacts
Which extraction method is the most appropriate for a tissue sample? - ANSWER-
Organic
Which extraction method is possibly too harsh for a very old, degraded bloodstain? -
ANSWER-Chelex
Which extraction method is the most appropriate for a vaginal swab from a sexual
assault kit? - ANSWER-Differential
Which extraction method is the most appropriate for a buccal swab (reference
standard)? - ANSWER-Either Chelex or Organic
, What is the main type of buffer used in the organic extraction? - ANSWER-Digest Buffer
What is the purpose of the phenol in the phenol:chloroform organic extraction? -
ANSWER-Solubalize polysaccharides
The Chelex resin is selective for______? - ANSWER-Heavy metals and cations
Hairs that have been shed from the head (naturally fallen) are of what category? -
ANSWER-Telogen
What color blood tubes are preferred to use if DNA extraction is necessary? - ANSWER-
Purple
Generally chelex extraction is used in the recovery of DNA from oral samples. However,
due to the nature of one type of sample organ ic extraction may be considered to provide
better or more reliable results. Which sample type is it? - ANSWER-Chewing gum
True or False: The yield gel only measures total human DNA. - ANSWER-False
Nucleic acids have UVVis absorption characteristics at which wavelength range? -
ANSWER-240-280 nm
True or False The slot blot method of DNA quantitation can measure both double and
single-stranded DNA? - ANSWER-True
RT-PCR utilizes a modification of what? - ANSWER-Polymerase Chain Reaction
What type of equipment is necessary to use for RT-PCR? - ANSWER-Thermal cycler
True or False? Multiplex PCR uses more than one set of PCR primers to target multiple
locations. - ANSWER-True
True or False The target amount of extracted DNA necessary for PCR is in the region of
0.5 2 µg. - ANSWER-False
The short polynucleotide chains used in PCR to target regions of amplification are
called? - ANSWER-Primers
In what organism is Taq Polymerase found? - ANSWER-Thermus Aquaticus
What is the purpose of EDTA during the PCR reaction? - ANSWER-chelator
DNA fragments will travel - ANSWER-Toward the anode and away from the cathode
How many STR loci can you get information about using Profiler and Cofiler kits? -
ANSWER-13
By using the Profiler and Cofiler Kits how
many amplifications does it take to obtain information on all the CODIS loci? - ANSWER-
2
Typical data applied to an electropherogram does NOT include - ANSWER-Peak Area
But it does include: name of locus, number of repeats, size of fragment, and peak
height.
What does the genescan software NOT do? - ANSWER-Assigns allele calls to the peaks
Assuming two donors, consider one locus with alleles 12, 13 and 14. How many
different ALLELIC combinations are possible for one of the individuals in the mixture? -
ANSWER-6
True or False: You can analyze your data without an allelic ladder? - ANSWER-False
What is a NOT a part of the ABI 310 Genetic Analyzer? - ANSWER-Chiller
Parts include: Capillary, CCD camera, syringe with polymer
What is the name for the information collected directly from the CCD camera? -
ANSWER-Raw data
ANSWER-Alec Jeffreys
RFLP is ____ sensitive as PCR methods. - ANSWER-less
Which method uses the smallest fragments of DNA? - ANSWER-STR
CODIS looks for relationships between the profiles of unidentified remains and relatives
of missing persons. - ANSWER-True
short tandem repeats (STRs) are also known as _____ - ANSWER-Microsatellites
Which is not in DAB standard 6? - ANSWER-D. Amplified DNA does not need to be in
separate area than DNA extraction and PCR set-up.
When pipetting the pipet tip should be placed - ANSWER-at the top of the liquid sample
If you are LEFT handed, the order of movement would be as follows: - ANSWER-Left to
right
Which is NOT appropriate during sample preparation? - ANSWER-Process both
question samples and reference samples together.
What is NOT considered a type of eye protection? - ANSWER-Contacts
Which extraction method is the most appropriate for a tissue sample? - ANSWER-
Organic
Which extraction method is possibly too harsh for a very old, degraded bloodstain? -
ANSWER-Chelex
Which extraction method is the most appropriate for a vaginal swab from a sexual
assault kit? - ANSWER-Differential
Which extraction method is the most appropriate for a buccal swab (reference
standard)? - ANSWER-Either Chelex or Organic
, What is the main type of buffer used in the organic extraction? - ANSWER-Digest Buffer
What is the purpose of the phenol in the phenol:chloroform organic extraction? -
ANSWER-Solubalize polysaccharides
The Chelex resin is selective for______? - ANSWER-Heavy metals and cations
Hairs that have been shed from the head (naturally fallen) are of what category? -
ANSWER-Telogen
What color blood tubes are preferred to use if DNA extraction is necessary? - ANSWER-
Purple
Generally chelex extraction is used in the recovery of DNA from oral samples. However,
due to the nature of one type of sample organ ic extraction may be considered to provide
better or more reliable results. Which sample type is it? - ANSWER-Chewing gum
True or False: The yield gel only measures total human DNA. - ANSWER-False
Nucleic acids have UVVis absorption characteristics at which wavelength range? -
ANSWER-240-280 nm
True or False The slot blot method of DNA quantitation can measure both double and
single-stranded DNA? - ANSWER-True
RT-PCR utilizes a modification of what? - ANSWER-Polymerase Chain Reaction
What type of equipment is necessary to use for RT-PCR? - ANSWER-Thermal cycler
True or False? Multiplex PCR uses more than one set of PCR primers to target multiple
locations. - ANSWER-True
True or False The target amount of extracted DNA necessary for PCR is in the region of
0.5 2 µg. - ANSWER-False
The short polynucleotide chains used in PCR to target regions of amplification are
called? - ANSWER-Primers
In what organism is Taq Polymerase found? - ANSWER-Thermus Aquaticus
What is the purpose of EDTA during the PCR reaction? - ANSWER-chelator
DNA fragments will travel - ANSWER-Toward the anode and away from the cathode
How many STR loci can you get information about using Profiler and Cofiler kits? -
ANSWER-13
By using the Profiler and Cofiler Kits how
many amplifications does it take to obtain information on all the CODIS loci? - ANSWER-
2
Typical data applied to an electropherogram does NOT include - ANSWER-Peak Area
But it does include: name of locus, number of repeats, size of fragment, and peak
height.
What does the genescan software NOT do? - ANSWER-Assigns allele calls to the peaks
Assuming two donors, consider one locus with alleles 12, 13 and 14. How many
different ALLELIC combinations are possible for one of the individuals in the mixture? -
ANSWER-6
True or False: You can analyze your data without an allelic ladder? - ANSWER-False
What is a NOT a part of the ABI 310 Genetic Analyzer? - ANSWER-Chiller
Parts include: Capillary, CCD camera, syringe with polymer
What is the name for the information collected directly from the CCD camera? -
ANSWER-Raw data
