Lecture ma
Entiatiian
DNA
transcription
RNA
translation
protein
DNAreplication
Thecomplementarybasemustbe added
in
iii
errors in DNA replication result in mutations
semiconservative the new DNAmolecule
consistsof one andone daughto parentalstrandacts as atemplatefor
rarefied ranga
p gugu makingnew complementary strand
enzymes involved
i HelicaseunzipsDNAfromrightto left
separates strandsby breakingdown it bonds holdingbasepairstog
parent
movesdown DNA andunzips DNAusingATP
strand
sina.gg
trandbindingproteins preventsbasesfrom rebinding
replication fork
are 2 topoisomerase breaks supercoil worksupstreamfromreplication
fork to relievestress that resultsfromunwinding helix at replication
Fritmase
3 makes RNA primer DNA polymerase linksnew nucleotides
ligasejoins ligate DNAfragments
DNApolymerasecanonlyattach anucleotidetoanothernucleotide canonlyelongatetheend
of anexistingpieceof DNAorRNA so eachnewDNAstrand mustbegin with a shortstretch
of Rna rnaprimgrnqmaptgent.si Dnastrand
pripiiet
once nucleotidesareadded anotherDNApolymerase removesprimer and replaces it
antiparallel 5 3 3 s withDNA ligasejoinsDNAfragments
DNAsynthesis
DNApolymerase canonly add nucleotides to the 3 endof anothernucleotide
If DNAsynthesis occursonlyin 51 31
nucleotides bases ononestrandhydrogenbonds to complementary
nucleotides on otherstrand
I 39999onttiititiain.fi onas triphosphate
OHgroupattachedto c canaccess innermost phosphatebondof 3Pa Theoxygen
attacks thebond seizes an e bondbreaks
breakinghigh energy phosphate bondprovides energy todrivereaction that
creates new phosphodiester bondattaching theincoming nucleotide to the endofthe
growingchain duringDNAsynthesis thelagging
because DNAreplication occurs s 3 onestrand is strandhastoform a loop to
strand
synthesizedcontinuously leading synchronizeDNA synthesison both
otherstrandis laggingstrand synthesizedinpieces okazaki É
r.ee strands
fragments newRNAprimer hastobelaiddownforeachpiecenencemornogester loopgrowsduringokazaki
fragmentsynthesis and collapses
uponcompletion allowing
ming polymerasetorelease rebindto
p
lagging new primer repeat
leading
Entiatiian
DNA
transcription
RNA
translation
protein
DNAreplication
Thecomplementarybasemustbe added
in
iii
errors in DNA replication result in mutations
semiconservative the new DNAmolecule
consistsof one andone daughto parentalstrandacts as atemplatefor
rarefied ranga
p gugu makingnew complementary strand
enzymes involved
i HelicaseunzipsDNAfromrightto left
separates strandsby breakingdown it bonds holdingbasepairstog
parent
movesdown DNA andunzips DNAusingATP
strand
sina.gg
trandbindingproteins preventsbasesfrom rebinding
replication fork
are 2 topoisomerase breaks supercoil worksupstreamfromreplication
fork to relievestress that resultsfromunwinding helix at replication
Fritmase
3 makes RNA primer DNA polymerase linksnew nucleotides
ligasejoins ligate DNAfragments
DNApolymerasecanonlyattach anucleotidetoanothernucleotide canonlyelongatetheend
of anexistingpieceof DNAorRNA so eachnewDNAstrand mustbegin with a shortstretch
of Rna rnaprimgrnqmaptgent.si Dnastrand
pripiiet
once nucleotidesareadded anotherDNApolymerase removesprimer and replaces it
antiparallel 5 3 3 s withDNA ligasejoinsDNAfragments
DNAsynthesis
DNApolymerase canonly add nucleotides to the 3 endof anothernucleotide
If DNAsynthesis occursonlyin 51 31
nucleotides bases ononestrandhydrogenbonds to complementary
nucleotides on otherstrand
I 39999onttiititiain.fi onas triphosphate
OHgroupattachedto c canaccess innermost phosphatebondof 3Pa Theoxygen
attacks thebond seizes an e bondbreaks
breakinghigh energy phosphate bondprovides energy todrivereaction that
creates new phosphodiester bondattaching theincoming nucleotide to the endofthe
growingchain duringDNAsynthesis thelagging
because DNAreplication occurs s 3 onestrand is strandhastoform a loop to
strand
synthesizedcontinuously leading synchronizeDNA synthesison both
otherstrandis laggingstrand synthesizedinpieces okazaki É
r.ee strands
fragments newRNAprimer hastobelaiddownforeachpiecenencemornogester loopgrowsduringokazaki
fragmentsynthesis and collapses
uponcompletion allowing
ming polymerasetorelease rebindto
p
lagging new primer repeat
leading
