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Lab notes BIOL 151 (BIO151)

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GM plants, Using PCR to detect GM data, molecular techniques in lab, PCR, Gel Electrophoresis, Detecting DNA, and basic local alignment search tool (BLAST)

Institution
University Of Wisconsin - Madison
Course
BIOL 151 (BIO151)

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omo analysisPCR
Ynu e's
introducing a specificDNAsequenceinto aplant'sgenometo produce a desiredtrait
exonethathasresistenceto herbicide
geneis linked toregulatoryDNAsequencesincludingpromoterthatensuresgeneisexpressedin modifiedplantc ells
commonlyusedpromoter camu35spromoter actsasnonswitch for geneexpression in manyplant species
cellsthatsuccessfullyincorporatetheDNAareselectedandregenintoconplants can reproduce traits areinherited
usingPCRto detect onDNA
poramplifiesDnasequence
bydesigningprimersthatbindto specificDNAsequences cangeneratemillionsofcopiesofthose sequences ifthey'representin
extractedDNA
percanonlydetectdatathatis
presentinthesample
intacte nough to amplify
notinhibitedbysubstances in theextraction
geneofinterestisnottargetforourPcrprimer useprimers specificto35spromoter sequence
positivecontrol oncorn w constructthus35spromoter
negativecontrol wildtypecornwhoconstructthusno 35spromoter
iftubulingeneis successfullyamplified
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Institution
University Of Wisconsin - Madison
Course
BIOL 151 (BIO151)
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April 22, 2026
Number of pages
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Carol lee
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