TEST BANK FOR HISTOTECHNOLOGY HTL COMPREHENSIVE EXAM | ACTUAL QUESTIONS AND VERIFIED ANSWERS | GRADED A+ | FIRST ATTEMPT|BRAND NEW 2026 UPDATE!!!!!!

Alcian Blue demonstrates - ANSWER Acid mucins



ATPase Enyme Histochemistry demonstrates - ANSWER ATPase in muscles



Bielschowsky's Silver Technique demonatrates - ANSWER Dendrites, axons, neurofibrils



Brown & Brenn gram stain demonstrates - ANSWER bacteria



Congo red demonstrates - ANSWER amyloid



Cresyl Echt Violet demonstrates - ANSWER Nissl substance



Geimsa stain demonstrates - ANSWER mast cells or H. pylori



Gomori Burtner demonstrates - ANSWER melanin



Gomori's Aldehyde Fuchsin

demonstrates - ANSWER elastic and mast cells



Gordon & Sweet's demonstrates - ANSWER reticulin



Grocott's Methenamine Silver demonstrates - ANSWER fungi (eg. Pneumocystis)




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,Hematoxylin & Eosin shows - ANSWER general stain



Immunohistotochemistry - ANSWER Anything an antibody can be made for



Luxol fast blue stain shows - ANSWER myelin



Toluidine blue stain shows - ANSWER mast cells or H. pylory



Masson's Trichrome shows - ANSWER collagen



Oil Red O technique shows - ANSWER neutral fats (simple fats)



Papanicolaou technique shows - ANSWER cytology smears



Periodic Acid Schiff's shows - ANSWER neutral mucin, glycogen



Perl's Prussian Blue shows - ANSWER Hemosiderin



Southgate's mucicarmine - ANSWER epithelial acid mucins



Verhoeff's Van Gieson - ANSWER elastic



Van Kossa shows - ANSWER calcium



Ziehl Neelsen shows - ANSWER Acid fast bacilli (T.B.)




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,Nuclei in Gomori's Aldehyde Fuchsin technique - ANSWER yellow - no specific stain for
nuclei



Mast cells in a Gomori's Aldehyde Fuchsin technique - ANSWER purple - GAF not specific
for elastic



Gram positive bacteria if over-differentiated after crystal violet - ANSWER red



Nuclei in the Brown and Brenn Gram stain if over differentiated with picric acid/acetone -
ANSWER yellow



Fungi in the Periodic Acid Schiff (PAS) technique - ANSWER magenta - chitin binds with
Schiffs



Fungi in the Grocott's Methenamine Silver technique - ANSWER black - silver impregna-
tion



Neutral mucins in a PAS technique if potassium permanganate is used as the oxidizing agent
- ANSWER light pink - colour of background - CHO overoxidized from aldehydes to acid
state



Cytoplasm in an H&E if over differentiated with acid alcohol - ANSWER pink - only affects
nuclei



Epithelial acid mucins in the Southgate's mucicarmine technique - ANSWER red



Cytoplasm in an H&E if the blueing agent is not properly removed - ANSWER very pale
pink



Nuclei in the Congo Red technique - ANSWER blue - stained with aluminum hematoxylin

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, Calcium in the Von Kossa technique - ANSWER black - silver impregnation



Glycogen, after diastase in the Periodic Acid Schiff technique - ANSWER pale pink - back-
ground colour



Mycobacterium after Ziehl Neelsen technique - ANSWER red - AFB stain with carbol fuch-
sin



Mycobacterium after Ziehl Neelsen technique - ANSWER red - AFB stain with carbol fuch-
sin



Streptococcus after Ziehl Neelsen technique - ANSWER blue - colour of background



Neutral lipids for Oil Red O cut on a cryostat - ANSWER red to orange



Neutral lipids for Oil Red O cut from wax block - ANSWER not demonstrated - removed



Hemosiderin after a Perl's Prussian Blue - ANSWER blue



Nuclei after Masson's trichrome using an aluminum Hematoxylin - ANSWER red - same as
cytoplasm



Fine elastic fibres in Verhoeff's Van Gieson (VVG) after leaving in Van Gieson reagent too long
- ANSWER not black/not demonstrated - iron hematoxylin removed by the acid



Cytoplasm if underdifferentiated in the VVG technique - ANSWER grey to black




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