Alcian Blue - ANSWER The demonstration of acid mucopolysaccharides
Alcian Blue-PAS - ANSWER To differentiate b/w neutral and acidic mucosubstances
detection of intestinal metaplasia
Alcian Yellow - ANSWER Alcian yellow is used in histology in conjunction with toluidine
blue to help visualize H. pylori
The resulting structures are stained as follows
- Mucin: yellow
- H. pylori: blue
- Other cellular components/background: shades of blue
Bielschowsky - ANSWER Demonstrate nerve fivers and the presence of neurofibrillary
tangles and senile plaques in Alzheimer disease
Carbol-fuchsin (Kinyoun, Ziehl-Neelsen, Fite, auramine-rhodamine) - ANSWER to detect
the presence of acid-fast mycobacteria in tissue sections
Colloidal Iron - ANSWER Identification of carboxylated and sulfated mucopolysaccharides
and glycoprotein mucin in tissue
Congo Red - ANSWER Demonstration of amyloid in tissues
1
, Crystal Violet - ANSWER Rapid screening method for amyloid but is not as specific as
Congo red
Fontana-Masson - ANSWER The demonstration of argentaffin substances such as mela-
nin, argentaffin granules of carcinoid tumors, and some neurosecretory granules
Neuroendocrine cells, neuroendocrine tumors, and melanoma
Giemsa - ANSWER to permit differentiation of cells present in hematopoietic tissue.
also used for the demonstration of some microorganisms
Gram - ANSWER identification of Gram-positive and Gram-negative microorganisms in
tissue samples
Grocott/Gomori methenamine-silver (GMS) - ANSWER demonstration of urates in tissue.
A disturbance of uric-acid metabolism known as gout can cause the deposition of urate crys-
tals, especially around joints and in soft tissues.
Collection of the urate crystals are known as gouty tophi
Melanin bleach - ANSWER Bleaching with potassium permanganate or hydrogen perox-
ide is used to remove melanin to examine cellular morphology
Hematoxylin & Eosin (H&E) - ANSWER Stain nucleus blue/purple
Extracellular matrix/cytoplasm pink
2
Alcian Blue-PAS - ANSWER To differentiate b/w neutral and acidic mucosubstances
detection of intestinal metaplasia
Alcian Yellow - ANSWER Alcian yellow is used in histology in conjunction with toluidine
blue to help visualize H. pylori
The resulting structures are stained as follows
- Mucin: yellow
- H. pylori: blue
- Other cellular components/background: shades of blue
Bielschowsky - ANSWER Demonstrate nerve fivers and the presence of neurofibrillary
tangles and senile plaques in Alzheimer disease
Carbol-fuchsin (Kinyoun, Ziehl-Neelsen, Fite, auramine-rhodamine) - ANSWER to detect
the presence of acid-fast mycobacteria in tissue sections
Colloidal Iron - ANSWER Identification of carboxylated and sulfated mucopolysaccharides
and glycoprotein mucin in tissue
Congo Red - ANSWER Demonstration of amyloid in tissues
1
, Crystal Violet - ANSWER Rapid screening method for amyloid but is not as specific as
Congo red
Fontana-Masson - ANSWER The demonstration of argentaffin substances such as mela-
nin, argentaffin granules of carcinoid tumors, and some neurosecretory granules
Neuroendocrine cells, neuroendocrine tumors, and melanoma
Giemsa - ANSWER to permit differentiation of cells present in hematopoietic tissue.
also used for the demonstration of some microorganisms
Gram - ANSWER identification of Gram-positive and Gram-negative microorganisms in
tissue samples
Grocott/Gomori methenamine-silver (GMS) - ANSWER demonstration of urates in tissue.
A disturbance of uric-acid metabolism known as gout can cause the deposition of urate crys-
tals, especially around joints and in soft tissues.
Collection of the urate crystals are known as gouty tophi
Melanin bleach - ANSWER Bleaching with potassium permanganate or hydrogen perox-
ide is used to remove melanin to examine cellular morphology
Hematoxylin & Eosin (H&E) - ANSWER Stain nucleus blue/purple
Extracellular matrix/cytoplasm pink
2
