STRAIGHTERLINE MICROBIOLOGY LAB
BIO250L LAB TWO CULTURING ASEPTIC
TECHNIQUE PRACTICE SCRIPT 2026
QUESTIONS WITH SOLUTIONS GRADED A+
⩥ Negative Stain. Answer:
⩥ Acid-Fast Stain. Answer:
⩥ Capsule Stain. Answer:
⩥ Endospore Stain. Answer:
⩥ Wet Mount and Hanging Drop. Answer: allow observation of living
cells to determine motility
⩥ Flagella Stain. Answer: (prepared stain) allows for observation of
flagella
⩥ Columbia CNA with 5% Sheep Blood Agar. Answer:
differential/selective medium that allows for growth of Gram-positive
and inhibit growth of Gram-negative organisms
,⩥ Know the Microscopes Parts!. Answer:
⩥ Base. Answer: supports the microscope
⩥ Mechanical Stage. Answer:
⩥ Condenser. Answer: focuses light through the specimen
⩥ Arm. Answer: along with the base, used to support the microscope
when carried
⩥ Objective Lens. Answer: have magnification ranging from 4x to 40x
⩥ Light Source/Illuminator. Answer: projects light upward through the
diaphragm and stage
⩥ Diaphragm. Answer: regulates the amount of light on the specimen
⩥ Ocular Lens. Answer: has a magnification of 10x.; aka eyepiece
⩥ Revolving Nosepiece. Answer: rotates the objective lenses
, ⩥ Stage Clips. Answer: holds the slide in place
⩥ Slide. Answer: where the specimen is placed
⩥ Specimen. Answer: the object being studied
⩥ Coarse Adjustment Knob. Answer: moves the stage up and down for
focusing
⩥ Fine Adjustment Knob. Answer: moves the stage slightly to sharpen
the image
⩥ Know the proper procedure for focusing the microscope:. Answer: 1.
On the low-power objective, use the coarse objective only
2. When higher power objectives are used, the fine adjustment knob can
be changed to bring the object into focus.
⩥ Know the proper procedure when viewing a slide:. Answer: 1. turn on
microscope
2. Choose slide
3. Put it on stage
4. Lock the slide in place
BIO250L LAB TWO CULTURING ASEPTIC
TECHNIQUE PRACTICE SCRIPT 2026
QUESTIONS WITH SOLUTIONS GRADED A+
⩥ Negative Stain. Answer:
⩥ Acid-Fast Stain. Answer:
⩥ Capsule Stain. Answer:
⩥ Endospore Stain. Answer:
⩥ Wet Mount and Hanging Drop. Answer: allow observation of living
cells to determine motility
⩥ Flagella Stain. Answer: (prepared stain) allows for observation of
flagella
⩥ Columbia CNA with 5% Sheep Blood Agar. Answer:
differential/selective medium that allows for growth of Gram-positive
and inhibit growth of Gram-negative organisms
,⩥ Know the Microscopes Parts!. Answer:
⩥ Base. Answer: supports the microscope
⩥ Mechanical Stage. Answer:
⩥ Condenser. Answer: focuses light through the specimen
⩥ Arm. Answer: along with the base, used to support the microscope
when carried
⩥ Objective Lens. Answer: have magnification ranging from 4x to 40x
⩥ Light Source/Illuminator. Answer: projects light upward through the
diaphragm and stage
⩥ Diaphragm. Answer: regulates the amount of light on the specimen
⩥ Ocular Lens. Answer: has a magnification of 10x.; aka eyepiece
⩥ Revolving Nosepiece. Answer: rotates the objective lenses
, ⩥ Stage Clips. Answer: holds the slide in place
⩥ Slide. Answer: where the specimen is placed
⩥ Specimen. Answer: the object being studied
⩥ Coarse Adjustment Knob. Answer: moves the stage up and down for
focusing
⩥ Fine Adjustment Knob. Answer: moves the stage slightly to sharpen
the image
⩥ Know the proper procedure for focusing the microscope:. Answer: 1.
On the low-power objective, use the coarse objective only
2. When higher power objectives are used, the fine adjustment knob can
be changed to bring the object into focus.
⩥ Know the proper procedure when viewing a slide:. Answer: 1. turn on
microscope
2. Choose slide
3. Put it on stage
4. Lock the slide in place
