Biochem 501 Exam 3 Questions And
Answers 2026 Update!!!
Activated Carrier of electron NADH/FADH2
Cells receive, process and respond to info in
Signal transduction
their environment
The symbol deltaGº represents while deltaG
a standard reference point; actual conditions
represents:
Which of the following statements regarding
Gibbs free energy is correct:
a. if delta G is negative then delta Gº will also be
negative
b. if a reaction has a large and negative deltaGº b is correct. If a reaction has a negative deltaG-
product formation will be favored in actual then product formation will be favored. This can
conditions unless the ratio of P:R is high enough change if the concentration of the products is
to drive the reverse reaction. really high, shifting the reaction to the reactants
c. if the reaction has a large and negative
deltaGº the enzyme prefers to form reactants
d. if a reaction has a large and positive deltaG,
product formation will be favored
Consider the reaction A --> B. If at equilibrium,
Negative since the products are favored and the
the [B] > [A], is the change in deltaGº of the
reaction is spontaneous
reaction: negative, positive or zero
Which of the following molecules allosterically
ATP; it serves as an inhibitor of PFK-1 because it
inhibits phosphofructokinase?
signals a high E state in the cell. Under this state,
ADP there is enough ATP so the regulated step of
AMP PFK-1 is inhibited and the ATP production of
glycolysis can be slowed
ATP
, Steps 1, 3, and 10 of glycolysis are irreversible. b. these energies are in fact large and NEGATIVE
Which of the following statements is since they are coupled to ATP hydrolysis.
INCORRECT?
a. these steps are catalyzed by kinases (add a
phosphate)
b. The deltaGº for these steps are large and
positive
c. cellular conditions do not exist that allow
these steps to proceed in the other direction
How many pyruvate molecules are formed from
2; 6C glucose --> 2x3C pyruvate
one molecule of glucose?
2; 1 ATP used in step 1 and 1 ATP used in step 3.
How many net ATP are formed in glycolysis per
ATP is produced in step 7 and step 10 where it
glucose molecule?
produces 2 molecules each time. 4-2 = 2
Which of the following statements regarding
fermentation is correct?
a. alcohol DH regenerates NADH for glycolysis
d. in anaerobic conditions, pyruvate can be
b. Fermentation occurs more readily in aerobic
converted to lactate via lactate DH, reducing
conditions than in anaerobic conditions
pyruvate to lactate and oxidizing NADH to
c. glucose consumption is lower in anaerobic NAD+ to be fed back into glycolysis
conditions than in aerobic conditions
d. the lactate DH reaction regenerates NAD+ for
glycolysis
Where is the PDH complex located? mitochondria
Consider the PDH complex. When is CO2 E1 reactions - this is a decarboxylation reaction
formed in the PDH reaction mechanism? in which CO2 is released
Which of the following is a product of the PDH NADH -formed in the E3 reactions; energy is
complex? stored in this molecule
How many reduced bonds does acetate have? In
other words, how many reduced cofactors are
4
formed when acetate is fully oxidized in the
CAC?
Answers 2026 Update!!!
Activated Carrier of electron NADH/FADH2
Cells receive, process and respond to info in
Signal transduction
their environment
The symbol deltaGº represents while deltaG
a standard reference point; actual conditions
represents:
Which of the following statements regarding
Gibbs free energy is correct:
a. if delta G is negative then delta Gº will also be
negative
b. if a reaction has a large and negative deltaGº b is correct. If a reaction has a negative deltaG-
product formation will be favored in actual then product formation will be favored. This can
conditions unless the ratio of P:R is high enough change if the concentration of the products is
to drive the reverse reaction. really high, shifting the reaction to the reactants
c. if the reaction has a large and negative
deltaGº the enzyme prefers to form reactants
d. if a reaction has a large and positive deltaG,
product formation will be favored
Consider the reaction A --> B. If at equilibrium,
Negative since the products are favored and the
the [B] > [A], is the change in deltaGº of the
reaction is spontaneous
reaction: negative, positive or zero
Which of the following molecules allosterically
ATP; it serves as an inhibitor of PFK-1 because it
inhibits phosphofructokinase?
signals a high E state in the cell. Under this state,
ADP there is enough ATP so the regulated step of
AMP PFK-1 is inhibited and the ATP production of
glycolysis can be slowed
ATP
, Steps 1, 3, and 10 of glycolysis are irreversible. b. these energies are in fact large and NEGATIVE
Which of the following statements is since they are coupled to ATP hydrolysis.
INCORRECT?
a. these steps are catalyzed by kinases (add a
phosphate)
b. The deltaGº for these steps are large and
positive
c. cellular conditions do not exist that allow
these steps to proceed in the other direction
How many pyruvate molecules are formed from
2; 6C glucose --> 2x3C pyruvate
one molecule of glucose?
2; 1 ATP used in step 1 and 1 ATP used in step 3.
How many net ATP are formed in glycolysis per
ATP is produced in step 7 and step 10 where it
glucose molecule?
produces 2 molecules each time. 4-2 = 2
Which of the following statements regarding
fermentation is correct?
a. alcohol DH regenerates NADH for glycolysis
d. in anaerobic conditions, pyruvate can be
b. Fermentation occurs more readily in aerobic
converted to lactate via lactate DH, reducing
conditions than in anaerobic conditions
pyruvate to lactate and oxidizing NADH to
c. glucose consumption is lower in anaerobic NAD+ to be fed back into glycolysis
conditions than in aerobic conditions
d. the lactate DH reaction regenerates NAD+ for
glycolysis
Where is the PDH complex located? mitochondria
Consider the PDH complex. When is CO2 E1 reactions - this is a decarboxylation reaction
formed in the PDH reaction mechanism? in which CO2 is released
Which of the following is a product of the PDH NADH -formed in the E3 reactions; energy is
complex? stored in this molecule
How many reduced bonds does acetate have? In
other words, how many reduced cofactors are
4
formed when acetate is fully oxidized in the
CAC?
