Microbio Lab Midterm BIOL 2051
Brininstool Questions Solved
Correctly.
complex medium - Answer composed of digest of chemically undefined substances
defined medium - Answer precise chemical composition is known
agar is extracted from ... - Answer Rhodophyta (red algae)
after heating to 100 deg, it will solidify at 40-42 deg and not remelt until it reaches ____ deg -
Answer 80-90
plates are primarily used for... - Answer isolation of microorganisms, streak plates
deeps are used for ________ growth - Answer anaerobic
slants are used for.. - Answer maintenance of stock cultures
How do sterilization and pasteurization differ? - Answer sterilization is killing or removal of
all living organisms and viruses. involves high heat and pressure via autoclaving. pasteurization
involves gentle heating
what can kill endospores? - Answer autoclave
an autoclave is heated to ___deg C at ___ psi for at least __ min - Answer 121, 15, 15
how would you remove bacteria from beer? - Answer filtration
what type of sterilization uses electromagnetic radiation? - Answer cold sterilization
what type of sterilization uses ethylene oxide? - Answer cold sterilization
what type of sterilization uses UV radiation to create thymine dimers? - Answer cold
sterilization
, what type of sterilization is used on plastic objects such as Petri dishes? - Answer cold
sterilization
how does dry heat sterilization kill bacteria? - Answer through oxidation effects
what is the most effective sterilization technique that is used in labs? - Answer dry heat
sterilization
the membrane of a filter is typically composed of... - Answer cellulose acetate or cellulose
resident microbiota - Answer normal flora non pathenogenic. replicate. can become
pathenogenic when introduced inside the body
transient flora - Answer pathogenic. can't live on skin bc of acidity. do not replicate.
how does the skin repel bacteria? - Answer with its acidity
how does hand-washing affect the presence of microbiota? - Answer eliminates potentially
transient microorganisms, sloughs off dead skin which has resident microbiota
assuming no mutation, the cells in a colony have different genetic compositions T/F - Answer
false
purpose of heat fixing - Answer kill cells, destroy enzymes, cells adhere to slide, allows for
easier visualization
cells are __% water by weight and __% by volume - Answer 90, 70
prokaryotic cells only range in size from ____micrometers - Answer .2-.5
vibrious - Answer curved rods
4 cellular morphologies? - Answer cocci, bacilli, spiral, vibrious
prosthecatae - Answer cellular appendages that increase cell surface area and help transport
nutrients into the cell
Brininstool Questions Solved
Correctly.
complex medium - Answer composed of digest of chemically undefined substances
defined medium - Answer precise chemical composition is known
agar is extracted from ... - Answer Rhodophyta (red algae)
after heating to 100 deg, it will solidify at 40-42 deg and not remelt until it reaches ____ deg -
Answer 80-90
plates are primarily used for... - Answer isolation of microorganisms, streak plates
deeps are used for ________ growth - Answer anaerobic
slants are used for.. - Answer maintenance of stock cultures
How do sterilization and pasteurization differ? - Answer sterilization is killing or removal of
all living organisms and viruses. involves high heat and pressure via autoclaving. pasteurization
involves gentle heating
what can kill endospores? - Answer autoclave
an autoclave is heated to ___deg C at ___ psi for at least __ min - Answer 121, 15, 15
how would you remove bacteria from beer? - Answer filtration
what type of sterilization uses electromagnetic radiation? - Answer cold sterilization
what type of sterilization uses ethylene oxide? - Answer cold sterilization
what type of sterilization uses UV radiation to create thymine dimers? - Answer cold
sterilization
, what type of sterilization is used on plastic objects such as Petri dishes? - Answer cold
sterilization
how does dry heat sterilization kill bacteria? - Answer through oxidation effects
what is the most effective sterilization technique that is used in labs? - Answer dry heat
sterilization
the membrane of a filter is typically composed of... - Answer cellulose acetate or cellulose
resident microbiota - Answer normal flora non pathenogenic. replicate. can become
pathenogenic when introduced inside the body
transient flora - Answer pathogenic. can't live on skin bc of acidity. do not replicate.
how does the skin repel bacteria? - Answer with its acidity
how does hand-washing affect the presence of microbiota? - Answer eliminates potentially
transient microorganisms, sloughs off dead skin which has resident microbiota
assuming no mutation, the cells in a colony have different genetic compositions T/F - Answer
false
purpose of heat fixing - Answer kill cells, destroy enzymes, cells adhere to slide, allows for
easier visualization
cells are __% water by weight and __% by volume - Answer 90, 70
prokaryotic cells only range in size from ____micrometers - Answer .2-.5
vibrious - Answer curved rods
4 cellular morphologies? - Answer cocci, bacilli, spiral, vibrious
prosthecatae - Answer cellular appendages that increase cell surface area and help transport
nutrients into the cell
