HISTOLOGY 1 EXAM 1 QUESTIONS &
ANSWERS
define histology - Correct Answers -science concerned with the minute structure of
cells, tissues, and organs in relation to their function
what are the 4 fundamental tissues - Correct Answers -epithelial
connective
muscular
nervous
what are the basic steps of histology - Correct Answers -fixation
processing
cutting
mounting
staining
cover slipping/reading
what is the purpose of fixation - Correct Answers -to stop autolysis
prevent bacterial decomposition
stabilize proteins
what is used to fix the tissue the majority of the time - Correct Answers -formalin 10%
why is the dehydrates tissue put in paraffin - Correct Answers -makes it easier to cut the
tissue down
supravital stain - Correct Answers -only live cells with stain
what is the main way a stain forms - Correct Answers -from electrostatic charges ( acid
to base )
what is the name of the routine stain - Correct Answers -hematoxyline and eosin
hematoxylin - Correct Answers -basic, attaches to - charges and produces a purple
pigment. natural dye
eosin - Correct Answers -acidic, attaches to + charges and produces a pink pigment
eosinophilic - Correct Answers -stained by an acidic stain
, basophilic - Correct Answers -stained by a basic stain
what are the 3 orientations of organs/tissues for histology - Correct Answers -cross
section
longitudinal section
oblique or tangential section
what are serial sections and why are they helpful - Correct Answers -slides of nearly
identical tissue. it allows you to use a variety of stains to examine the tissue
what is used to estimate size of what is seen on slides - Correct Answers -based on
approx size of red blood cells (erythrocyte)
define artifacts - Correct Answers -when something goes wrong due to processing or
poor tissue quality
what is the purpose of trichrome stains - Correct Answers -help distinguish tissue/cell
type. pink is epithelial, red is muscle, blue is connective
what is seen in orcein staining - Correct Answers -elastic fibers appear dark
brown/black
what is the purpose of toluidine blue staining - Correct Answers -confirm mast cell
tumors
what is the purpose of prussian blue staining - Correct Answers -shows iron in blue
what is hemochromatosis - Correct Answers -an excess of iron in the liver that can kill
the liver tissue
what is the purpose of congo red staining - Correct Answers -it is a polarizer, makes the
accumulation of amyloid appear bright green
what do the colors represent in gram staining - Correct Answers -positive - purple
negative - pink
what is the purpose of the ziehl neelsen stain - Correct Answers -to identify acid-fast
bacteria (TB)
what is the purpose of silver stains - Correct Answers -the fungal wall appears black
what is the purpose of periodic acid schiff staining (PAS) - Correct Answers -stains
polysaccharides pink
what is the purpose of counterstaining and what is typically used - Correct Answers -
hematoxylin is used to stain the nuclei and structure of the cell a purple/blue color
ANSWERS
define histology - Correct Answers -science concerned with the minute structure of
cells, tissues, and organs in relation to their function
what are the 4 fundamental tissues - Correct Answers -epithelial
connective
muscular
nervous
what are the basic steps of histology - Correct Answers -fixation
processing
cutting
mounting
staining
cover slipping/reading
what is the purpose of fixation - Correct Answers -to stop autolysis
prevent bacterial decomposition
stabilize proteins
what is used to fix the tissue the majority of the time - Correct Answers -formalin 10%
why is the dehydrates tissue put in paraffin - Correct Answers -makes it easier to cut the
tissue down
supravital stain - Correct Answers -only live cells with stain
what is the main way a stain forms - Correct Answers -from electrostatic charges ( acid
to base )
what is the name of the routine stain - Correct Answers -hematoxyline and eosin
hematoxylin - Correct Answers -basic, attaches to - charges and produces a purple
pigment. natural dye
eosin - Correct Answers -acidic, attaches to + charges and produces a pink pigment
eosinophilic - Correct Answers -stained by an acidic stain
, basophilic - Correct Answers -stained by a basic stain
what are the 3 orientations of organs/tissues for histology - Correct Answers -cross
section
longitudinal section
oblique or tangential section
what are serial sections and why are they helpful - Correct Answers -slides of nearly
identical tissue. it allows you to use a variety of stains to examine the tissue
what is used to estimate size of what is seen on slides - Correct Answers -based on
approx size of red blood cells (erythrocyte)
define artifacts - Correct Answers -when something goes wrong due to processing or
poor tissue quality
what is the purpose of trichrome stains - Correct Answers -help distinguish tissue/cell
type. pink is epithelial, red is muscle, blue is connective
what is seen in orcein staining - Correct Answers -elastic fibers appear dark
brown/black
what is the purpose of toluidine blue staining - Correct Answers -confirm mast cell
tumors
what is the purpose of prussian blue staining - Correct Answers -shows iron in blue
what is hemochromatosis - Correct Answers -an excess of iron in the liver that can kill
the liver tissue
what is the purpose of congo red staining - Correct Answers -it is a polarizer, makes the
accumulation of amyloid appear bright green
what do the colors represent in gram staining - Correct Answers -positive - purple
negative - pink
what is the purpose of the ziehl neelsen stain - Correct Answers -to identify acid-fast
bacteria (TB)
what is the purpose of silver stains - Correct Answers -the fungal wall appears black
what is the purpose of periodic acid schiff staining (PAS) - Correct Answers -stains
polysaccharides pink
what is the purpose of counterstaining and what is typically used - Correct Answers -
hematoxylin is used to stain the nuclei and structure of the cell a purple/blue color
