BIOCHEMISTRY CERTIFICATION TEST
BANK PREP 2026 QUESTIONS WITH
SOLUTIONS GRADED A+
◉An uncatalyzed reaction has a rate of 4.2 x 10 -7 sec -1 . When an
enzyme is added the rate is 3.2
x 10 4 sec -1 . Calculate the rate enhancement caused by the enzyme.
A) 7.6 x 10^10
B) 3.2 x 10^4
C) 1.3 x 10^-2
D) 7.4 x 10^-3
E) cannot be determined ANSWER:-A) 7.6 x 10^10
◉Which of the following is the most effective way to increase the rate
of a biochemical
reaction?
A) increase the temperature
B) increase the concentrations of the reactants
C) increase or decrease the pH
D) add a catalyst
E) none of the above ANSWER:-D) add a catalyst
,◉Chymotrypsin catalyzes the hydrolysis of a peptide bond and is
therefore categorized as a
_____.
A) oxidoreductase
B) transferase
C) hydrolase
D) lyase
E) ligase ANSWER:-C) hydrolase
◉The ability for an enzyme to pick out one particular substrate from the
myriad of molecules
floating around its environment is an example of _____.
A) natural selection
B) specificity
C) high affinity
D) processivity
E) none of the above ANSWER:-B) specificity
◉The highest point in a reaction coordinate diagram represents _____.
A) an intermediate of the reaction pathway
B) the reactants in an exergonic reaction
C) the products in an endergonic reaction
D) the transition state
E) the overall deltaG for the reaction ANSWER:-D) the transition state
,◉How does a catalyst affect the overall deltaG of an endergonic
reaction?
A) it has no effect
B) the reaction becomes more endergonic
C) the reaction has a deltaG of zero
D) the reaction becomes exergonic
E) none of the above ANSWER:-A) it has no effect
◉How does a catalyst increase the rate of a reaction?
A) it makes the reaction more exergonic
B) it increases the temperature of the reaction
C) it allows reacting molecules to more easily form the transition state
D) it causes a localized increase in the concentration of reactants
E) none of the above ANSWER:-C) it allows reacting molecules to more
easily form the transition state
◉An organic molecule that is tightly bound to an enzyme and
participates in an enzyme
catalyzed reaction is specifically referred to as a _____.
A) cofactor
B) metal ion
C) coenzyme
D) cosubstrate
, E) prosthetic group ANSWER:-E) prosthetic group
◉An enzyme that forms a covalent bond with its substrate during the
course of a reaction is
considered to undergo _____.
A) acid-base catalysis
B) covalent catalysis
C) electrophilic catalysis
D) metal ion catalysis
E) none of the above ANSWER:-B) covalent catalysis
◉Which of the following amino acids would be most likely found in the
active site of an
enzyme that uses acid-base catalysis?
A) Asn
B) Ser
C) Met
D) His
E) Trp ANSWER:-D) His
◉Which of the following is seen in a reaction coordinate diagram for an
enzyme-catalyzed
reaction that uses covalent catalysis?
A) an intermediate
BANK PREP 2026 QUESTIONS WITH
SOLUTIONS GRADED A+
◉An uncatalyzed reaction has a rate of 4.2 x 10 -7 sec -1 . When an
enzyme is added the rate is 3.2
x 10 4 sec -1 . Calculate the rate enhancement caused by the enzyme.
A) 7.6 x 10^10
B) 3.2 x 10^4
C) 1.3 x 10^-2
D) 7.4 x 10^-3
E) cannot be determined ANSWER:-A) 7.6 x 10^10
◉Which of the following is the most effective way to increase the rate
of a biochemical
reaction?
A) increase the temperature
B) increase the concentrations of the reactants
C) increase or decrease the pH
D) add a catalyst
E) none of the above ANSWER:-D) add a catalyst
,◉Chymotrypsin catalyzes the hydrolysis of a peptide bond and is
therefore categorized as a
_____.
A) oxidoreductase
B) transferase
C) hydrolase
D) lyase
E) ligase ANSWER:-C) hydrolase
◉The ability for an enzyme to pick out one particular substrate from the
myriad of molecules
floating around its environment is an example of _____.
A) natural selection
B) specificity
C) high affinity
D) processivity
E) none of the above ANSWER:-B) specificity
◉The highest point in a reaction coordinate diagram represents _____.
A) an intermediate of the reaction pathway
B) the reactants in an exergonic reaction
C) the products in an endergonic reaction
D) the transition state
E) the overall deltaG for the reaction ANSWER:-D) the transition state
,◉How does a catalyst affect the overall deltaG of an endergonic
reaction?
A) it has no effect
B) the reaction becomes more endergonic
C) the reaction has a deltaG of zero
D) the reaction becomes exergonic
E) none of the above ANSWER:-A) it has no effect
◉How does a catalyst increase the rate of a reaction?
A) it makes the reaction more exergonic
B) it increases the temperature of the reaction
C) it allows reacting molecules to more easily form the transition state
D) it causes a localized increase in the concentration of reactants
E) none of the above ANSWER:-C) it allows reacting molecules to more
easily form the transition state
◉An organic molecule that is tightly bound to an enzyme and
participates in an enzyme
catalyzed reaction is specifically referred to as a _____.
A) cofactor
B) metal ion
C) coenzyme
D) cosubstrate
, E) prosthetic group ANSWER:-E) prosthetic group
◉An enzyme that forms a covalent bond with its substrate during the
course of a reaction is
considered to undergo _____.
A) acid-base catalysis
B) covalent catalysis
C) electrophilic catalysis
D) metal ion catalysis
E) none of the above ANSWER:-B) covalent catalysis
◉Which of the following amino acids would be most likely found in the
active site of an
enzyme that uses acid-base catalysis?
A) Asn
B) Ser
C) Met
D) His
E) Trp ANSWER:-D) His
◉Which of the following is seen in a reaction coordinate diagram for an
enzyme-catalyzed
reaction that uses covalent catalysis?
A) an intermediate
