Which is the detector of choice for nephelometers?
Select one:
a. diode array
b. photomultiplier tube
c. gas photodiode
d. LED - Correct Answer b
Which statement regarding turbidimetry is NOT correct?
Select one:
a. light scattered by solutes in the solution at 180° from the incident light is measured by a photon
detector
b. the measurement of light is made at right angles to the incident light
c. scattered light is directly proportional to the number of particles in solution
d. measures the amount of incident light blocked by particles - Correct Answer b
What is the effect on the colligative properties when you increase the number of particles in the
solution?
Select one:
a. Increases the boiling point, increases the freezing point
b. Increases the boiling point, increases the vapor pressure
c. Decreases the boiling point, increases the freezing point,
d. Decreases the freezing point, decreases the vapor pressure - Correct Answer d
- increase boiling point
- decrease freezing point
- decrease vapor pressure
- increase osmotic pressure
,What is the definition of retention factor (Rf) in thin layer chromatography?
Select one:
a. Ratio of distance moved by the unknown solute to distance moved by the solvent in the mobile phase
b. Rate of movement of the mobile phase through adsorbant compared with standards and controls
c. Measurement in centimeters of the distance the solute moved in the mobile phase from the point of
application
d. Distance moved by the mobile phase front from the point of application compared with a control -
Correct Answer a
What is quenching?
Select one:
a. a type of resonant fluorescence
b. an analytical procedure for determining fluorescent compounds.
c. the loss of fluorescent intensity which produces falsely decreased patient results.
d. the loss of fluorescent intensity which produces falsely increased patient results. - Correct Answer c
What will cause false values when using nephelometric analysis?
Select one:
a. absorption by a closely related atomic species
b. excess analyte in the antigen/antibody reaction
c. hemolyzed sample
d. too much fluorophore in the analyte/fluorescing compound mixture - Correct Answer b
What measurement describes a current flow between two non-polarized electrodes with a known
electrical potential?
Select one:
a. amperometry
b. conductometry
c. coulometry
d. potentiometry - Correct Answer b
,What does aldosterone secretion lead to?
Select one:
a. hypotension
b. increased ECF (extracellular fluid) volume
c. increased serum potassium
d. none of the above - Correct Answer b
Which of these factors DO NOT influence the rate of an enzyme reaction?
Select one:
a. enzyme concentration
b. substrate concentration
c. cofactors
d. nomenclature - Correct Answer d
Which statement regarding serum urea is correct?
Select one:
a. is elevated in prerenal and renal failure
b. it is not reabsorbed by the renal tubules
c. high levels can result from necrotic liver disease
d. levels are independent of diet - Correct Answer a
Which of the following dyes is the most specific for the measurement of albumin?
Select one:
a. tetrabromosulfophthalein
b. bromcresol green
c. bromcresol purple
d. tetrabromphenol blue - Correct Answer c
, Which of the following lipid results would be expected to be falsely elevated on a serum specimen from
a non-fasting patient?
Select one:
a. Cholesterol
b. HDL
c. LDL
d. Triglyceride - Correct Answer d
What is the major consequence of acetaminophen poisoning?
Select one:
a. Seizures
b. Hyperventilation
c. Hepatocystic necrosis
d. Renal failure - Correct Answer c
What is the most common method to measure hormone levels in the blood?
Select one:
a. electrophoresis
b. immunoassays
c. photometry
d. gas chromatography - Correct Answer b
Question text
You are examining a protein electrophoresis pattern and notice an unusual band at the starting point of
the electropherogram that is not present in the normal control. If the patient was healthy, what is the
most likely cause of the unusual banding pattern?
Select one:
a. a plasma sample was probably applied instead of serum
b. the buffer used was the wrong pH
c. an inappropriate support medium was used
Select one:
a. diode array
b. photomultiplier tube
c. gas photodiode
d. LED - Correct Answer b
Which statement regarding turbidimetry is NOT correct?
Select one:
a. light scattered by solutes in the solution at 180° from the incident light is measured by a photon
detector
b. the measurement of light is made at right angles to the incident light
c. scattered light is directly proportional to the number of particles in solution
d. measures the amount of incident light blocked by particles - Correct Answer b
What is the effect on the colligative properties when you increase the number of particles in the
solution?
Select one:
a. Increases the boiling point, increases the freezing point
b. Increases the boiling point, increases the vapor pressure
c. Decreases the boiling point, increases the freezing point,
d. Decreases the freezing point, decreases the vapor pressure - Correct Answer d
- increase boiling point
- decrease freezing point
- decrease vapor pressure
- increase osmotic pressure
,What is the definition of retention factor (Rf) in thin layer chromatography?
Select one:
a. Ratio of distance moved by the unknown solute to distance moved by the solvent in the mobile phase
b. Rate of movement of the mobile phase through adsorbant compared with standards and controls
c. Measurement in centimeters of the distance the solute moved in the mobile phase from the point of
application
d. Distance moved by the mobile phase front from the point of application compared with a control -
Correct Answer a
What is quenching?
Select one:
a. a type of resonant fluorescence
b. an analytical procedure for determining fluorescent compounds.
c. the loss of fluorescent intensity which produces falsely decreased patient results.
d. the loss of fluorescent intensity which produces falsely increased patient results. - Correct Answer c
What will cause false values when using nephelometric analysis?
Select one:
a. absorption by a closely related atomic species
b. excess analyte in the antigen/antibody reaction
c. hemolyzed sample
d. too much fluorophore in the analyte/fluorescing compound mixture - Correct Answer b
What measurement describes a current flow between two non-polarized electrodes with a known
electrical potential?
Select one:
a. amperometry
b. conductometry
c. coulometry
d. potentiometry - Correct Answer b
,What does aldosterone secretion lead to?
Select one:
a. hypotension
b. increased ECF (extracellular fluid) volume
c. increased serum potassium
d. none of the above - Correct Answer b
Which of these factors DO NOT influence the rate of an enzyme reaction?
Select one:
a. enzyme concentration
b. substrate concentration
c. cofactors
d. nomenclature - Correct Answer d
Which statement regarding serum urea is correct?
Select one:
a. is elevated in prerenal and renal failure
b. it is not reabsorbed by the renal tubules
c. high levels can result from necrotic liver disease
d. levels are independent of diet - Correct Answer a
Which of the following dyes is the most specific for the measurement of albumin?
Select one:
a. tetrabromosulfophthalein
b. bromcresol green
c. bromcresol purple
d. tetrabromphenol blue - Correct Answer c
, Which of the following lipid results would be expected to be falsely elevated on a serum specimen from
a non-fasting patient?
Select one:
a. Cholesterol
b. HDL
c. LDL
d. Triglyceride - Correct Answer d
What is the major consequence of acetaminophen poisoning?
Select one:
a. Seizures
b. Hyperventilation
c. Hepatocystic necrosis
d. Renal failure - Correct Answer c
What is the most common method to measure hormone levels in the blood?
Select one:
a. electrophoresis
b. immunoassays
c. photometry
d. gas chromatography - Correct Answer b
Question text
You are examining a protein electrophoresis pattern and notice an unusual band at the starting point of
the electropherogram that is not present in the normal control. If the patient was healthy, what is the
most likely cause of the unusual banding pattern?
Select one:
a. a plasma sample was probably applied instead of serum
b. the buffer used was the wrong pH
c. an inappropriate support medium was used
