Ch. 8 RNA synthesis and processing questions and Answers updated 2026

Ch. 8 RNA synthesis and processing
questions and Answers updated
2026
TranscriptionBisB_______-dependentB_______Bsynthesis.

a.BDNA;BDNA

b.BDNA;BRNA

c.BRNA;BDNA

d.BRNA;BproteinB-BAnswerb.BDNA;BRNA



TheBprokaryoticBcellsBmostBcommonlyBusedBtoBstudyBtranscriptionBandBtranslationBare

from

a.BE.Bcoli.

b.BB.Bsubtilis.

c.BS.Bcerevisiae.

d.BD.Bdiscoidium.B-BAnswera.BE.Bcoli.



RNABpolymeraseBdiffersBfromBDNABpolymeraseBinBthatBit

a.BsynthesizesBnewBstrandsBofBRNABinBaB3′BtoB5′Bdirection.

b.BisBaBmonomericBprotein.

c.BcanBsynthesizeBaBcomplementaryBstrandBwithoutBtheBtwoBstrandsBofBDNABbeing

separated.

d.BdoesBnotBrequireBaBprimerBtoBinitiateBsynthesisBofBRNA.B-
BAnswerd.BdoesBnotBrequireBaBprimerBtoBinitiateBsynthesisBofBRNA.



TheBDNABsequenceBtoBwhichBanBRNABpolymeraseBbindsBtoBinitiateBtranscriptionBofBa

geneBisBcalledBa(n)

a.Benhancer.

b.Bpromoter.

c.Bpolymerase-bindingBelement.

d.BoriginBofBtranscription.B-BAnswerb.Bpromoter.

,TheBregionsBofBtheBDNABwhereBRNABpolymeraseBbindsBcanBbeBidentifiedBby

a.BrestrictionBmapping.

b.BPCR.

c.BDNABfootprinting.

d.BpolymeraseBmapping.B-BAnswerc.BDNABfootprinting.



TheBroleBofBtheBsigmaB(σ)BfactorBinBprokaryoteBtranscriptionBisBto

a.BunwindBtheBDNABduringBtranscription.

b.BprovideBspecificityBtoBbindingBtoBtheBpromoterBregion.

c.BrecognizeBtheBtranscriptionBinitiationBsite.

d.BterminateBtranscription.B-BAnswerc.BrecognizeBtheBtranscriptionBinitiationBsite.



TranscriptionBofBaBbacterialBgeneBisBinitiated

a.BwithBRNABpolymeraseBinBtheBclosedBpromoterBcomplex.

b.BafterBreleaseBofBtheBsigmaB(σ)Bfactor

c.BwithBRNABpolymeraseBinBtheBopenBpromoterBcomplex.

d.BBothBaBandBbB-BAnswerc.BwithBRNABpolymeraseBinBtheBopenBpromoterBcomplex.



TerminationBofBtranscriptionBinBE.BcoliBoccursBby

a.BformationBofBaBstem-loopBstructureBinBtheBRNA.

b.BbindingBofBRhoBproteinBtoBtheBendBofBtheBmRNA.

c.BbindingBofBaBsigmaB(σ)BfactorBtoBtheBendBofBtheBmRNA.

d.BEitherBaBorBbB-BAnswerd.BEitherBaBorBb



TheBpioneeringBstudiesBofBgeneBregulationBinBE.BcoliBwereBdoneBinBtheB1950sBby

a.BMillerBandBUrey.

b.BWatsonBandBCrick.

c.BJacobBandBMonod.

d.BLernerBandBLowe.B-BAnswerc.BJacobBandBMonod



OneBwouldBexpectBtheBmRNABproducedBfromBtheBlacBoperonBofBE.BcoliBtoBhybridizeBto

, the

a.BlacBi+Bgene.

b.BlacBoBregion.

c.Bβ-galactosidaseBgene.

d.BAllBofBtheBaboveB-BAnswerb.BlacBoBregion.



TheBlacBoperonBinBE.BcoliBisBregulatedBbyBlactose,BwhichB_______Ba(n)B_______Bof

transcription.

a.Bactivates;Bactivator

b.Binactivates;Bactivator

c.Bactivates;Brepressor

d.Binactivates;BrepressorB-BAnswerd.Binactivates;Brepressor



TheBlacBoperatorBconsistsBofBapproximatelyB20BbaseBpairsBofBDNABlocated

a.B80-100BbaseBpairsBupstreamBofBtheBtranscriptionBinitiationBsite.

b.B20-40BbaseBpairsBupstreamBofBtheBtranscriptionBinitiationBsite.

c.BinBaBpositionBoverlappingBtheBinitiationBsite.

d.BdownstreamBofBtheBtranscriptionBinitiationBsite.B-BAnswerb.B20-
40BbaseBpairsBupstreamBofBtheBtranscriptionBinitiationBsite.



WhenBtheBoperatorBgeneBhasBanBoperatorBconstitutiveBmutationB(oc

),BtheBgene(s)

regulatedBbyBitBare

a.BoffBwhenBtheyBshouldBbeBon.

b.BalwaysBoff.

c.BalwaysBon.

d.BregulatedBbyBtheBpresenceBofBtheBrepressor.B-BAnswerc.BalwaysBon.



CataboliteBrepressionBinBE.Bcoli

a.BinhibitsBtheBbreakdownBofBglucoseBinBtheBpresenceBofBlactose.

b.BisBreversedBbyBcAMPBbindingBtoBcataboliteBactivatorBprotein.

c.BinhibitsBtheBlacBoperonBunlessBlactoseBisBpresent.