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Microbiology Final Exam|Microorganisms, Germs, Aseptic Technique, Spontaneous Generation, Biogenesis, Hooke, Leeuwenhoek, Redi, Needham, Spallanzani, Virchow, Pasteur, Lister, Koch, Semmelweis, Jenner, Fleming, Ehrlich, Lancefield, Berg, Linnaeus, Pasteur

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Microbiology Final Exam|Microorganisms, Germs, Aseptic Technique, Spontaneous Generation, Biogenesis, Hooke, Leeuwenhoek, Redi, Needham, Spallanzani, Virchow, Pasteur, Lister, Koch, Semmelweis, Jenner, Fleming, Ehrlich, Lancefield, Berg, Linnaeus, Pasteurization, Vaccines, Synthetic Drugs, Antibiotics, Quinine, Salversan, Sulfa Drugs, Emerging Infectious Diseases, Epidemiology, Pathology, Microbiota, Symbiosis, Opportunistic Pathogens Exam Questions Verified and Provided with Complete A+ Graded Rationales Latest Updated 2026 What are microorganisms? What are germs? Microorganisms are organisms that are too small to be seen with the unaided eye. Germ refers to a rapidly growing cell. What is aseptic technique and why is it important? Prevents contamination in medicine and microbiology laboratories, in turn, preventing the spread of infectious diseases. What is spontaneous generation? The hypothesis that living organisms arise from nonliving matter; a "vital force" forms life. What is biogenesis? The hypothesis that living organisms arise from preexisting life. What scientists believed in spontaneous generation? John Needham What scientists believed in biogenesis? Francesco Redi Rudolf Virchow Lazzaro Spallanzani Louis Pasteur Robert Hook Developed microscope Beginning of Cell theory (all living things are composed of cells or little boxes) Cork cells were boxes Anton Van Leeuwenhoek Amateur scientist Observed microbes "animicules" Detailed drawings Francesco Redi Sealed and unsealed jars Net covered and uncovered jars Maggots do not arise spontaneously from decaying meat Flies lay eggs which become maggots John Needham Bacteria from cooked broth Showed bacteria in broth after being boiled Vital force in air (when shut lids to broth containers) Oxygen = vital force (Lavoisier) Lazzaro Spallanzani Nutrient broth placed in flask, heated, then sealed. Resulted in no microbial growth. Rudolf Virchow Living cells can only arise from preexisting living cells. Coined the term biogenesis. Louis Pasteur Demonstrated that microorganisms are present in the air. Nutrient broth placed in flask, heated, not sealed resulted in microbial growth. Nutrient broth placed in flask, heated, then sealed resulted in no microbial growth. Joseph Lister Used a chemical disinfectant to prevent surgical wound infections. Robert Koch Demonstrated that a bacterium causes anthrax and provided the experimental steps, Koch’s postulates, to prove that a specific microbe causes a specific disease. Ignaz Semmelweis Staphylococcus aureus Handwashing and childbed (puerperal) fever.

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Institution
Advance Nursing
Course
Advance nursing

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Microbiology Final Exam|Microorganisms, Germs, Aseptic
Technique, Spontaneous Generation, Biogenesis, Hooke,
Leeuwenhoek, Redi, Needham, Spallanzani, Virchow, Pasteur, Lister,
Koch, Semmelweis, Jenner, Fleming, Ehrlich, Lancefield, Berg,
Linnaeus, Pasteurization, Vaccines, Synthetic Drugs, Antibiotics,
Quinine, Salversan, Sulfa Drugs, Emerging Infectious Diseases,
Epidemiology, Pathology, Microbiota, Symbiosis, Opportunistic
Pathogens Exam Questions Verified and Provided with Complete A+
Graded Rationales Latest Updated 2026


What are microorganisms? What are germs?

Microorganisms are organisms that are too small to be seen with the unaided eye.



Germ refers to a rapidly growing cell.




What is aseptic technique and why is it important?

Prevents contamination in medicine and microbiology laboratories, in turn, preventing the spread of
infectious diseases.




What is spontaneous generation?

The hypothesis that living organisms arise from nonliving matter; a "vital force" forms life.




What is biogenesis?

The hypothesis that living organisms arise from preexisting life.

,What scientists believed in spontaneous generation?

John Needham




What scientists believed in biogenesis?

Francesco Redi

Rudolf Virchow

Lazzaro Spallanzani

Louis Pasteur




Robert Hook

Developed microscope

Beginning of Cell theory

(all living things are composed of cells or little boxes)

Cork cells were boxes




Anton Van Leeuwenhoek

Amateur scientist

Observed microbes

"animicules"

Detailed drawings




Francesco Redi

Sealed and unsealed jars

Net covered and uncovered jars

,Maggots do not arise spontaneously from decaying meat

Flies lay eggs which become maggots




John Needham

Bacteria from cooked broth

Showed bacteria in broth after being boiled

Vital force in air (when shut lids to broth containers)

Oxygen = vital force (Lavoisier)




Lazzaro Spallanzani

Nutrient broth placed in flask, heated, then sealed. Resulted in no microbial growth.




Rudolf Virchow

Living cells can only arise from preexisting living cells. Coined the term biogenesis.




Louis Pasteur

Demonstrated that microorganisms are present in the air.

Nutrient broth placed in flask, heated, not sealed resulted in microbial growth.

Nutrient broth placed in flask, heated, then sealed resulted in no microbial growth.




Joseph Lister

Used a chemical disinfectant to prevent surgical wound infections.

, Robert Koch

Demonstrated that a bacterium causes anthrax and provided the experimental steps, Koch’s postulates,
to prove that a specific microbe causes a specific disease.




Ignaz Semmelweis

Staphylococcus aureus

Handwashing and childbed (puerperal) fever.




Edward Jenner

Scratched cowpox intentionally into subject.

The subject was then protected from smallpox.




Paul Ehrlich

Magic Bullet (1910) - a term that describes drugs that could destroy a pathogen without harming the
host.




Alexander Fleming

Fungus used by Alexander Fleming to ID bacteria. First antibiotic was a lab accident.




Rebecca Lancefield

Classify serotypes of Streptococci



Different antigens in cell walls

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