AORN Periop 101 – Perioperative Nursing, Anesthesia & Surgical Safety | 140 Exam Questions with Answers | Chamberlain University 2026

This document provides 140 AORN Periop 101 final exam practice questions with verified answers designed to support students preparing for perioperative nursing assessments and clinical competency evaluations. The content focuses on essential principles of perioperative patient care, surgical safety, anesthesia management, sterile technique, infection prevention, and operating room protocols. The material is structured in a clear question-and-answer format, enabling nursing students and perioperative trainees to review high-yield concepts commonly tested in perioperative certification exams and hospital training programs. The questions cover a wide range of critical perioperative topics including types and stages of anesthesia, malignant hyperthermia recognition and management, intraoperative patient monitoring, perioperative patient positioning, sterile field maintenance, surgical draping techniques, wound classifications, surgical specimen handling, hemostasis methods, and operating room environmental controls. Additional topics include perioperative documentation requirements, surgical fire risks, latex hypersensitivity reactions, skin injury prevention, PACU recovery assessment using the Aldrete scale, and infection control practices such as hand hygiene and OR air pressure standards. These topics reflect the competencies required for safe surgical care and align with the core learning objectives of perioperative nursing education. The material closely aligns with the Association of periOperative Registered Nurses (AORN) Periop 101: A Core Curriculum™, one of the most widely used training programs for new operating room nurses. It also complements foundational perioperative textbooks such as “Alexander’s Care of the Patient in Surgery” and “Berry & Kohn’s Operating Room Technique”, which are commonly used in nursing schools and hospital-based perioperative training programs. The questions reinforce important clinical decision-making skills related to anesthesia care providers, intraoperative safety checks such as surgical time-out procedures, and sterile processing and sterilization protocols. This document is particularly useful for students enrolled in perioperative nursing, surgical nursing, operating room nursing, and clinical skills courses. It may benefit students studying in programs such as Perioperative Nursing Training, Surgical Technology, Operating Room Nursing Orientation Programs, RN Transition-to-OR Programs, and Nursing Clinical Practicums. It is especially relevant for nursing students or practicing nurses at institutions such as Chamberlain University, Walden University, Purdue University Global, Herzing University, Rasmussen University, and other nursing schools that incorporate AORN Periop 101 training as part of perioperative clinical education. The document also serves as a useful review resource for nurses preparing for CNOR certification or perioperative competency assessments in hospital surgical departments. Keywords: AORN periop 101 exam questions, perioperative nursing exam preparation, operating room nursing practice questions, surgical safety protocols review, anesthesia stages nursing exam, malignant hyperthermia management nursing, sterile technique perioperative nursing, surgical draping and sterile field guidelines, PACU Aldrete scale nursing review, perioperative patient positioning safety, surgical wound classification nursing, surgical specimen handling guidelines, infection control operating room nursing, surgical fire risk prevention nursing, perioperative documentation standards, OR environmental controls positive pressure air exchange, perioperative hemostasis methods review, latex hypersensitivity perioperative nursing, CNOR exam preparation questions, perioperative nursing clinical competency review