[BIOL 250] Lab Quiz #1 [EXP. #1, 2, 3, 5, & 6] UPDATED ACTUAL
Questions and CORRECT Answers
EXP. #1:
Aseptic technique Procedures that prevent microbes from getting where they do not belong
(contamination). This includes microbes from the environment contami- nating
your lab area as well as any microbes under study contaminating you or your work
area.
Bibulous paper Specially prepared blotting paper that contains a minimum of loose paper fibers.
Eukaryotic Cells with a true nucleus and paired chromosomes.
Parfocal Feature of most microscopes that sets the focal point of all objective lenses at the
same location in space
Prokaryotic Cells with no true nucleus (membrane covered, paired chromosomes), with only one
circular chromosome.
Resolution The ability to see a small object clearly under the microscope (technically, minimal
distance at which two adjacent small objects can be distinguished as separate).
Rheostat Device that controls the amount of electrical current and thus the amount of light
emanating from a bulb.
EXP. #2:
Agar Solidifying agent used for growth media derived from seaweed.
Agar deep Growth medium in a test tube allowed to solidify as the tube sits in a test-tube
rack.
Agar plate Solidified growth medium in a lid-covered, flat dish.
Agar slant Solidified growth medium in a test tube, allowed to solidify at an angle, thus
presenting a large surface area to allow for growth.
Autoclave Device that uses pressurized (15 lbs/in2) steam at 121°C to kill all known infectious
agents in 15 minutes.
Broth General term for liquid growth medium.
Colony A visible clump of bacteria growing on an agar plate, separated from other areas
of growth.
Questions and CORRECT Answers
EXP. #1:
Aseptic technique Procedures that prevent microbes from getting where they do not belong
(contamination). This includes microbes from the environment contami- nating
your lab area as well as any microbes under study contaminating you or your work
area.
Bibulous paper Specially prepared blotting paper that contains a minimum of loose paper fibers.
Eukaryotic Cells with a true nucleus and paired chromosomes.
Parfocal Feature of most microscopes that sets the focal point of all objective lenses at the
same location in space
Prokaryotic Cells with no true nucleus (membrane covered, paired chromosomes), with only one
circular chromosome.
Resolution The ability to see a small object clearly under the microscope (technically, minimal
distance at which two adjacent small objects can be distinguished as separate).
Rheostat Device that controls the amount of electrical current and thus the amount of light
emanating from a bulb.
EXP. #2:
Agar Solidifying agent used for growth media derived from seaweed.
Agar deep Growth medium in a test tube allowed to solidify as the tube sits in a test-tube
rack.
Agar plate Solidified growth medium in a lid-covered, flat dish.
Agar slant Solidified growth medium in a test tube, allowed to solidify at an angle, thus
presenting a large surface area to allow for growth.
Autoclave Device that uses pressurized (15 lbs/in2) steam at 121°C to kill all known infectious
agents in 15 minutes.
Broth General term for liquid growth medium.
Colony A visible clump of bacteria growing on an agar plate, separated from other areas
of growth.
