Blood Bank Exam 4 Component
Preparation- COMPLETE EXAM STUDY
GUIDE 2026/2027 WITH VERIFIED
ANSWERS | NEWEST VERSION
When did the first blood bank open?
1932 by Dr. Drew
When was the plastic blood bag invented?
1950
- Allowed for centrifugation and separation of individual blood components from whole blood
What do anticoagulant preservative solutions do for blood?
Prevent clotting factor activation
- Citrate-based, bind calcium and prevent coagulation cascade
- Maintain blood in liquid state
- Extend shelf life
What are the 5 approved anticoagulant preservation solutions?
1) Sodium citrate: CPD, CP2D, ACD, CPDA-1
2) Citric acid
3) Dextrose
4) Monobasic sodium phosphate: CPD, CP2D, CPDA-1
5) Adenine: CPDA-1
Constituents of anticoagulant preservation solutions
What does sodium citrate do?
Binds calcium and prevents coagulation cascade
,Constituents of anticoagulant preservation solutions
What does dextrose do?
Source of sugar for cell metabolism: feeds cells
Constituents of anticoagulant preservation solutions
What does adenine do?
Maintains ATP levels during cell metabolism
Constituents of anticoagulant preservation solutions
What does citric acid do?
Creates an acidic environment to slow down glycolysis that occurs through cell metabolism
What is the shelf life of ACD, CPD, and CP2D?
21 days
What is the shelf life for CPDA-1?
35 days
Once a bag is open, how long is the shelf life? (opened system)
24 hours
Production of blood components from WB
- What bag is the blood collected in?
- Satellite bag use
WB collected from donor is placed in collection bag containing anticoagulant preservative
- Satellite bags: storage containers for blood components prepared from donation (plasma, plts)
What does the closed system ensure?
No foreign contaminants from outside environment can enter system
What is whole blood comprised of?
- Is it used for transfusion?
- Benefit of separating WB into components?
All plasma and cellular components of blood
- Not used for transfusion typically
,- Separating whole blood into components allows multiple patients to benefit from a single
donation
What is the WB volume and HCT % in a WB donation?
WB volume: 450-500 mL
HCT: ~38%
Packed RBCs are prepared by:
- During centrifugation, ____ concentrate at the bottom of the container, followed by _____
and ____.
- What is in the top portion of the centrifuged collection bag?
Centrifuging whole blood
- RBCs concentrate at bottom; followed by WBC and platelet
- Top portion contains liquid plasma
After centrifugation, packed RBC unit is placed into _____.
Plasma press: slowly push out plasma
- RBCs and plasma bags separated using tube sealer
What is the final volume, HCT %, and Hgb of packed RBCS?
- What about if an additive is used?
- Volume: ~ 225-350mL
- HCT: ~65-80%
- Hgb: 50-80 grams
If additive is used, the final volume is around 300-400 mL, and the HCT is 55-65%
Additive solution extends shelf life to what?
42 days
Hgb is expected to (increase/decrease) by ____ g/dL for nonactive bleeders
Increase by 1 g/dl
Plasma is stored frozen to preserve activity of labile plasma proteins ____________
Factors V and VIII
, How to thaw plasma?
- ____ unit of each coag factor in every ____ mL of FFP
- WB derived plasma volume?
Frozen plasma is thawed prior to transfusion: water bath
- Stored in thawed state for short periods of time
- 1 unit of each coag factor in every 1 mL of FFP
- WB derived plasma volume: 200-300 mL
FFP processing and storage conditions
- Should be placed in freezer how long after collection?
- Once thawed, how long may it be stored?
- Stored frozen for how long?
Processed and placed in freezer within 8 hours of collection (or 6 for ACD: apheresis derived)
- Once thawed, may be stored for 24 hours (then relabeled as thawed plasma)
- Stored frozen for 12 months or for 7 years depending on temperature (7 years lower
temperature)
Plasma frozen (FP) within 24 hours (FP24) after phlebotomy (processing and collection
conditions)
- Should be placed in freezer how long after collection?
Processed and placed in freezer within 24 hours of collection
- Stored frozen
Plasma protein levels in FP or FP24
Levels of stable protein similar to that of FFP, except FVIII levels are somewhat reduced
Thawed plasma processing and collection conditions
- Prepared from what?
- Stored in fridge for up to how long?
Prepared from FFP/FP24
- Stored refrigerated for up to 5 days
Preparation- COMPLETE EXAM STUDY
GUIDE 2026/2027 WITH VERIFIED
ANSWERS | NEWEST VERSION
When did the first blood bank open?
1932 by Dr. Drew
When was the plastic blood bag invented?
1950
- Allowed for centrifugation and separation of individual blood components from whole blood
What do anticoagulant preservative solutions do for blood?
Prevent clotting factor activation
- Citrate-based, bind calcium and prevent coagulation cascade
- Maintain blood in liquid state
- Extend shelf life
What are the 5 approved anticoagulant preservation solutions?
1) Sodium citrate: CPD, CP2D, ACD, CPDA-1
2) Citric acid
3) Dextrose
4) Monobasic sodium phosphate: CPD, CP2D, CPDA-1
5) Adenine: CPDA-1
Constituents of anticoagulant preservation solutions
What does sodium citrate do?
Binds calcium and prevents coagulation cascade
,Constituents of anticoagulant preservation solutions
What does dextrose do?
Source of sugar for cell metabolism: feeds cells
Constituents of anticoagulant preservation solutions
What does adenine do?
Maintains ATP levels during cell metabolism
Constituents of anticoagulant preservation solutions
What does citric acid do?
Creates an acidic environment to slow down glycolysis that occurs through cell metabolism
What is the shelf life of ACD, CPD, and CP2D?
21 days
What is the shelf life for CPDA-1?
35 days
Once a bag is open, how long is the shelf life? (opened system)
24 hours
Production of blood components from WB
- What bag is the blood collected in?
- Satellite bag use
WB collected from donor is placed in collection bag containing anticoagulant preservative
- Satellite bags: storage containers for blood components prepared from donation (plasma, plts)
What does the closed system ensure?
No foreign contaminants from outside environment can enter system
What is whole blood comprised of?
- Is it used for transfusion?
- Benefit of separating WB into components?
All plasma and cellular components of blood
- Not used for transfusion typically
,- Separating whole blood into components allows multiple patients to benefit from a single
donation
What is the WB volume and HCT % in a WB donation?
WB volume: 450-500 mL
HCT: ~38%
Packed RBCs are prepared by:
- During centrifugation, ____ concentrate at the bottom of the container, followed by _____
and ____.
- What is in the top portion of the centrifuged collection bag?
Centrifuging whole blood
- RBCs concentrate at bottom; followed by WBC and platelet
- Top portion contains liquid plasma
After centrifugation, packed RBC unit is placed into _____.
Plasma press: slowly push out plasma
- RBCs and plasma bags separated using tube sealer
What is the final volume, HCT %, and Hgb of packed RBCS?
- What about if an additive is used?
- Volume: ~ 225-350mL
- HCT: ~65-80%
- Hgb: 50-80 grams
If additive is used, the final volume is around 300-400 mL, and the HCT is 55-65%
Additive solution extends shelf life to what?
42 days
Hgb is expected to (increase/decrease) by ____ g/dL for nonactive bleeders
Increase by 1 g/dl
Plasma is stored frozen to preserve activity of labile plasma proteins ____________
Factors V and VIII
, How to thaw plasma?
- ____ unit of each coag factor in every ____ mL of FFP
- WB derived plasma volume?
Frozen plasma is thawed prior to transfusion: water bath
- Stored in thawed state for short periods of time
- 1 unit of each coag factor in every 1 mL of FFP
- WB derived plasma volume: 200-300 mL
FFP processing and storage conditions
- Should be placed in freezer how long after collection?
- Once thawed, how long may it be stored?
- Stored frozen for how long?
Processed and placed in freezer within 8 hours of collection (or 6 for ACD: apheresis derived)
- Once thawed, may be stored for 24 hours (then relabeled as thawed plasma)
- Stored frozen for 12 months or for 7 years depending on temperature (7 years lower
temperature)
Plasma frozen (FP) within 24 hours (FP24) after phlebotomy (processing and collection
conditions)
- Should be placed in freezer how long after collection?
Processed and placed in freezer within 24 hours of collection
- Stored frozen
Plasma protein levels in FP or FP24
Levels of stable protein similar to that of FFP, except FVIII levels are somewhat reduced
Thawed plasma processing and collection conditions
- Prepared from what?
- Stored in fridge for up to how long?
Prepared from FFP/FP24
- Stored refrigerated for up to 5 days
