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MCAT Biochemistry Review | Test Exam Questions & Verified Answers | 2025 | 2026 | Graded A+

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MCAT Biochemistry Review | Test Exam Questions & Verified Answers | 2025 | 2026 | Graded A+ Quiz_________________? (S) Absolute Configuration - Answer All amino acids (except cysteine) have an (S) absolute configuration Quiz_________________? 1,3-Bisphosphoglycerate (1,3-BPG) and PEP - Answer High-energy intermediates used to generate ATP by substrate-level phosphorylation Only ATP gained in anaerobic respiration Quiz_________________? 2,4-dienoyl-CoA Reductase - Answer Converts 2 conjugated double bonds to just one double bond at the 3,4 position, where it will undergo the same rearrangement as monounsaturated fatty acids Quiz_________________? 3-Phosphoglycerate Kinase - Answer Transfers the high-energy phosphate from 1,3-bisphosphoglycerate to ADP, forming ATP and 3-phosphoglycerate Quiz_________________? 3' Poly-A Tail - Answer Posttranscriptional processing Polyadenosyl (poly-A) tail is added to the 3' end of the mRNA transcript and protects message from rapid degradation Assists with export of mature mRNA from nucleus Quiz_________________? 5' Cap - Answer Posttranscriptional processing a 7-methylguanylate triphosphate cap is added to the 5' end of the hnRNA molecule - recognized by ribosome as binding sight and protects mRNA from degradation in cytoplasm Quiz_________________? A Site - Answer Holds incoming aminoacyl-tRNA complex This is the amino acid being added next Quiz_________________? Absorption by Micelles - Answer Diffuse to brush border of intestinal mucosal cells and then absorbed - digested lipids pass through brush border - absorbed into mucosa - re-esterified to form triacylglycerols and cholesteryl esters - packaged with apoproteins, fat-soluble vitamins, and other lipids into chylomicrons - leave intestine via lacteals (vessels of the lymphatic system) - re-enter bloodstream via thoracic duct (long lymphatic vessel that empties into left subclavian vein) Shorter, water-soluble fatty acids can be absorbed directly into the bloodstream Quiz_________________? Acetals/Ketals - Answer Product of reaction between hemiacetals/hemiketals and alcohols Quiz_________________? Acetyl-CoA Carboxylase - Answer The rate-limiting step of fatty-acid synthesis Requires both biotin and ATP to function, and adds CO2 to acetyl-CoA to form malonyl-CoA Activated by insulin and citrate Incorporates acetyl-CoA into fatty acids Quiz_________________? Acetyl-CoA Shuttling - Answer Citrate diffuses across the mitochondrial membrane, where citrate lyase splits citrate into acetyl-CoA and oxaloacetate in the cytosol and oxaloacetate returns to the mitochodrion to continue moving acetyl-CoA Quiz_________________? Acetylation of Histone Proteins - Answer Decreases charge of lysine residues and weakens the interaction of histone with DNA, which opens up chromatin conformation and allows for easier access of transcriptional machinery to DNA

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Quiz_________________?

(S) Absolute Configuration -

Answer✅

All amino acids (except cysteine) have an (S) absolute configuration




Quiz_________________?

1,3-Bisphosphoglycerate (1,3-BPG) and PEP -

Answer✅

High-energy intermediates used to generate ATP by substrate-level phosphorylation

Only ATP gained in anaerobic respiration




Quiz_________________?

2,4-dienoyl-CoA Reductase -

Answer✅

Converts 2 conjugated double bonds to just one double bond at the 3,4 position, where it
will undergo the same rearrangement as monounsaturated fatty acids




Quiz_________________?


1

, 3-Phosphoglycerate Kinase -

Answer✅

Transfers the high-energy phosphate from 1,3-bisphosphoglycerate to ADP, forming ATP
and 3-phosphoglycerate




Quiz_________________?

3' Poly-A Tail -

Answer✅

Posttranscriptional processing

Polyadenosyl (poly-A) tail is added to the 3' end of the mRNA transcript and protects
message from rapid degradation

Assists with export of mature mRNA from nucleus




Quiz_________________?

5' Cap -

Answer✅

Posttranscriptional processing

a 7-methylguanylate triphosphate cap is added to the 5' end of the hnRNA molecule -
recognized by ribosome as binding sight and protects mRNA from degradation in cytoplasm




Quiz_________________?

A Site -

Answer✅

Holds incoming aminoacyl-tRNA complex

This is the amino acid being added next




2

, Quiz_________________?

Absorption by Micelles -

Answer✅

Diffuse to brush border of intestinal mucosal cells and then absorbed -> digested lipids pass
through brush border -> absorbed into mucosa -> re-esterified to form triacylglycerols and
cholesteryl esters -> packaged with apoproteins, fat-soluble vitamins, and other lipids into
chylomicrons -> leave intestine via lacteals (vessels of the lymphatic system) -> re-enter
bloodstream via thoracic duct (long lymphatic vessel that empties into left subclavian vein)

Shorter, water-soluble fatty acids can be absorbed directly into the bloodstream




Quiz_________________?

Acetals/Ketals -

Answer✅

Product of reaction between hemiacetals/hemiketals and alcohols




Quiz_________________?

Acetyl-CoA Carboxylase -

Answer✅

The rate-limiting step of fatty-acid synthesis

Requires both biotin and ATP to function, and adds CO2 to acetyl-CoA to form malonyl-
CoA

Activated by insulin and citrate

Incorporates acetyl-CoA into fatty acids




Quiz_________________?

Acetyl-CoA Shuttling -



3

, Answer✅

Citrate diffuses across the mitochondrial membrane, where citrate lyase splits citrate into
acetyl-CoA and oxaloacetate in the cytosol and oxaloacetate returns to the mitochodrion
to continue moving acetyl-CoA




Quiz_________________?

Acetylation of Histone Proteins -

Answer✅

Decreases charge of lysine residues and weakens the interaction of histone with DNA,
which opens up chromatin conformation and allows for easier access of transcriptional
machinery to DNA




Quiz_________________?

Actin (Structural) -

Answer✅

Makes up microfilaments and thin filaments in myofibrils

Most abundant protein in eukaryotic cells

Have + and - sides, allowing unidirectional movement




Quiz_________________?

Activation -

Answer✅

The conversion of a biomolecule to its active or usable form, such as activation of tRNA
with an amino acid or activation of a fatty acid with CoA to form fatty acyl-CoA




Quiz_________________?


4

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